Gene description for HTRA2
Gene name HtrA serine peptidase 2
Gene symbol HTRA2
Other names/aliases OMI
PARK13
PRSS25
Species Homo sapiens
 Database cross references - HTRA2
ExoCarta ExoCarta_27429
Vesiclepedia VP_27429
Entrez Gene 27429
HGNC 14348
MIM 606441
UniProt O43464  
 HTRA2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for HTRA2
Molecular Function
    protein binding GO:0005515 IPI
    unfolded protein binding GO:0051082 NAS
    peptidase activity GO:0008233 IDA
    serine-type endopeptidase activity GO:0004252 TAS
    serine-type peptidase activity GO:0008236 TAS
Biological Process
    regulation of mitochondrion degradation GO:1903146 TAS
    protein autoprocessing GO:0016540 TAS
    response to herbicide GO:0009635 IEA
    neuron development GO:0048666 IEA
    execution phase of apoptosis GO:0097194 TAS
    cellular response to interferon-beta GO:0035458 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    cellular response to heat GO:0034605 IDA
    regulation of multicellular organism growth GO:0040014 IEA
    proteolysis GO:0006508 TAS
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:2001269 IMP
    negative regulation of cell cycle GO:0045786 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IMP
    adult walking behavior GO:0007628 IEA
    mitochondrion organization GO:0007005 IEA
    cellular response to oxidative stress GO:0034599 NAS
    cellular response to growth factor stimulus GO:0071363 IMP
    forebrain development GO:0030900 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    cellular protein catabolic process GO:0044257 IDA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 NAS
    cellular response to retinoic acid GO:0071300 IDA
    positive regulation of cell death GO:0010942 IDA
    pentacyclic triterpenoid metabolic process GO:0019742 IEA
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IDA
    negative regulation of neuron death GO:1901215 TAS
    ceramide metabolic process GO:0006672 IEA
Subcellular Localization
    membrane GO:0016020 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytoplasmic side of plasma membrane GO:0009898 ISS
    endoplasmic reticulum GO:0005783 NAS
    mitochondrial intermembrane space GO:0005758 IDA
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IDA
    mitochondrial membrane GO:0031966 IEA
    mitochondrion GO:0005739 TAS
    CD40 receptor complex GO:0035631 ISS
    cytoskeleton GO:0005856 IDA
 Experiment description of studies that identified HTRA2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HTRA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DIABLO 56616
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 BIRC3 330
Reconstituted Complex Homo sapiens
3 XIAP  
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
4 MAPK14 1432
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which HTRA2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here