Gene description for CDK1
Gene name cyclin-dependent kinase 1
Gene symbol CDK1
Other names/aliases CDC2
CDC28A
P34CDC2
Species Homo sapiens
 Database cross references - CDK1
ExoCarta ExoCarta_983
Vesiclepedia VP_983
Entrez Gene 983
HGNC 1722
MIM 116940
UniProt P06493  
 CDK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
T lymphocytes 34108659    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for CDK1
Molecular Function
    virus receptor activity GO:0001618 IEA
    chromatin binding GO:0003682 IEA
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IBA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IMP
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IDA
    kinase activity GO:0016301 IDA
    cyclin binding GO:0030332 IDA
    cyclin binding GO:0030332 IPI
    Hsp70 protein binding GO:0030544 IEA
    histone kinase activity GO:0035173 IDA
    cyclin-dependent protein kinase activity GO:0097472 IDA
    protein serine kinase activity GO:0106310 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 kinase activity GO:0140833 IEA
    RNA polymerase II CTD heptapeptide repeat S2 kinase activity GO:0140834 IEA
    RNA polymerase II CTD heptapeptide repeat T4 kinase activity GO:0140835 IEA
    RNA polymerase II CTD heptapeptide repeat S5 kinase activity GO:0140836 IEA
    RNA polymerase II CTD heptapeptide repeat S7 kinase activity GO:0140837 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 NAS
    G2/M transition of mitotic cell cycle GO:0000086 IBA
    G2/M transition of mitotic cell cycle GO:0000086 IDA
    G2/M transition of mitotic cell cycle GO:0000086 NAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    microtubule cytoskeleton organization GO:0000226 TAS
    DNA replication GO:0006260 TAS
    DNA repair GO:0006281 TAS
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 IDA
    mitotic nuclear membrane disassembly GO:0007077 IDA
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IBA
    centrosome cycle GO:0007098 TAS
    pronuclear fusion GO:0007344 TAS
    response to xenobiotic stimulus GO:0009410 IEA
    response to toxic substance GO:0009636 IEA
    positive regulation of gene expression GO:0010628 IEA
    negative regulation of gene expression GO:0010629 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IMP
    regulation of Schwann cell differentiation GO:0014038 TAS
    response to amine GO:0014075 IEA
    response to activity GO:0014823 IEA
    cell migration GO:0016477 TAS
    protein deubiquitination GO:0016579 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    peptidyl-threonine phosphorylation GO:0018107 IMP
    chromosome condensation GO:0030261 IEA
    epithelial cell differentiation GO:0030855 IEP
    animal organ regeneration GO:0031100 IEA
    protein localization to kinetochore GO:0034501 IDA
    protein localization to kinetochore GO:0034501 IMP
    positive regulation of protein import into nucleus GO:0042307 IEA
    regulation of circadian rhythm GO:0042752 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    response to ethanol GO:0045471 IEA
    positive regulation of DNA replication GO:0045740 IEA
    regulation of embryonic development GO:0045995 TAS
    response to cadmium ion GO:0046686 IEA
    response to copper ion GO:0046688 IEA
    symbiont entry into host cell GO:0046718 IEA
    fibroblast proliferation GO:0048144 IEA
    rhythmic process GO:0048511 IEA
    response to axon injury GO:0048678 IEA
    cell division GO:0051301 IEA
    ventricular cardiac muscle cell development GO:0055015 IEA
    positive regulation of cardiac muscle cell proliferation GO:0060045 IEA
    positive regulation of mitotic sister chromatid segregation GO:0062033 IDA
    protein-containing complex assembly GO:0065003 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    ERK1 and ERK2 cascade GO:0070371 TAS
    cellular response to organic cyclic compound GO:0071407 IEA
    Golgi disassembly GO:0090166 ISS
    positive regulation of protein localization to nucleus GO:1900182 IMP
    regulation of attachment of mitotic spindle microtubules to kinetochore GO:1902423 IDA
    microtubule cytoskeleton organization involved in mitosis GO:1902850 IDA
    positive regulation of mitochondrial ATP synthesis coupled electron transport GO:1905448 IMP
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    chromosome, telomeric region GO:0000781 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 TAS
    mitochondrial matrix GO:0005759 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    spindle microtubule GO:0005876 IDA
    membrane GO:0016020 HDA
    midbody GO:0030496 IDA
    extracellular exosome GO:0070062 HDA
    mitotic spindle GO:0072686 IDA
    cyclin A1-CDK1 complex GO:0097121 NAS
    cyclin A2-CDK1 complex GO:0097122 NAS
    cyclin B1-CDK1 complex GO:0097125 IMP
    cyclin B1-CDK1 complex GO:0097125 IPI
 Experiment description of studies that identified CDK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
15
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
20
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
29
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
32
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
35
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
36
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
37
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
38
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
40
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
41
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
42
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
43
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
44
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
45
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
46
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
47
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CDK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Affinity Capture-Western Homo sapiens
2 PSMA6 5687
Affinity Capture-Western Homo sapiens
3 TLE1 7088
Biochemical Activity Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 CDCA2 157313
Biochemical Activity Homo sapiens
6 UBE2H 7328
Affinity Capture-MS Homo sapiens
7 FOXA1  
Affinity Capture-MS Homo sapiens
8 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
9 Cdk1 12534
Affinity Capture-MS Mus musculus
10 BOLA2 552900
Affinity Capture-MS Homo sapiens
11 GMNN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SPRTN  
Affinity Capture-MS Homo sapiens
13 CLIP1 6249
Affinity Capture-MS Homo sapiens
14 CDK3 1018
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 COIL  
Proximity Label-MS Homo sapiens
16 CCT5 22948
Affinity Capture-MS Homo sapiens
17 CCNB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 Dctn1 13191
Affinity Capture-MS Mus musculus
19 PTMA 5757
Co-purification Homo sapiens
20 SSBP1 6742
Biochemical Activity Homo sapiens
21 TFDP1  
Biochemical Activity Homo sapiens
22 GADD45G  
Reconstituted Complex Homo sapiens
23 GADD45A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 SP1  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
25 ALYREF 10189
Affinity Capture-MS Homo sapiens
26 E2F1 1869
Biochemical Activity Homo sapiens
27 GATA2  
Biochemical Activity Homo sapiens
28 EBNA-LP  
Affinity Capture-MS
29 SOX2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 BRCA1 672
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
31 MAP4 4134
Biochemical Activity Homo sapiens
32 CDC25B 994
Affinity Capture-Western Homo sapiens
33 Lmnb1  
Biochemical Activity Rattus norvegicus
34 HTRA2 27429
Biochemical Activity Homo sapiens
35 CSNK2A1 1457
Biochemical Activity Homo sapiens
36 IL16  
Biochemical Activity Homo sapiens
37 AAR2 25980
Affinity Capture-MS Homo sapiens
38 Rara  
Biochemical Activity Mus musculus
39 H1F0 3005
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
40 CCDC102B  
Affinity Capture-MS Homo sapiens
41 PLEKHG2 64857
Affinity Capture-MS Homo sapiens
42 PPP1CC 5501
Affinity Capture-Western Homo sapiens
43 HSPA4 3308
Affinity Capture-Western Homo sapiens
44 CCNK  
Co-fractionation Homo sapiens
45 PON1 5444
Biochemical Activity Homo sapiens
46 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
47 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
48 RNPS1 10921
Affinity Capture-MS Homo sapiens
49 NONO 4841
Biochemical Activity Homo sapiens
50 PRC1 9055
Biochemical Activity Homo sapiens
51 RBMXL2  
Affinity Capture-MS Homo sapiens
52 MAP1LC3B 81631
Affinity Capture-Western Homo sapiens
53 LYZ 4069
Affinity Capture-MS Homo sapiens
54 CCNF  
Affinity Capture-Western Homo sapiens
55 Cep55 74107
Affinity Capture-MS Mus musculus
56 FZR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
57 FBXW7  
Affinity Capture-MS Homo sapiens
58 AI837181  
Affinity Capture-MS Mus musculus
59 IQGAP1 8826
Biochemical Activity Homo sapiens
60 CUX1 1523
Biochemical Activity Homo sapiens
61 RGCC 28984
Biochemical Activity Homo sapiens
62 TMEM165 55858
Affinity Capture-MS Homo sapiens
63 SAFB 6294
Affinity Capture-MS Homo sapiens
64 MLST8 64223
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
65 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
66 ATP2A2 488
Affinity Capture-MS Homo sapiens
67 PRDX2 7001
Co-fractionation Homo sapiens
68 CCDC6 8030
Affinity Capture-Western Homo sapiens
69 DLG5 9231
Affinity Capture-MS Homo sapiens
70 IL3RA  
Two-hybrid Homo sapiens
71 RACGAP1 29127
Biochemical Activity Homo sapiens
72 XIAP  
Biochemical Activity Homo sapiens
73 MBP 4155
Biochemical Activity Homo sapiens
74 CDKN1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 USP45  
Affinity Capture-MS Homo sapiens
76 ITGA4 3676
Affinity Capture-MS Homo sapiens
77 CDT1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 VCAM1 7412
Affinity Capture-MS Homo sapiens
79 BUB1  
Biochemical Activity Homo sapiens
80 SFN 2810
Affinity Capture-Western Homo sapiens
81 CCNB3  
Affinity Capture-Western Homo sapiens
82 NSFL1C 55968
Biochemical Activity Homo sapiens
83 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
84 MYCN  
Affinity Capture-MS Homo sapiens
85 HRNR 388697
Affinity Capture-MS Homo sapiens
86 DLGAP5  
Affinity Capture-Western Homo sapiens
87 MARCKS 4082
Proximity Label-MS Homo sapiens
Biochemical Activity Homo sapiens
88 UIMC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
89 KIF14 9928
Affinity Capture-MS Homo sapiens
90 PCNA 5111
Affinity Capture-MS Homo sapiens
91 FEN1 2237
Reconstituted Complex Homo sapiens
92 SAMHD1 25939
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
93 USP22 23326
Affinity Capture-Western Homo sapiens
94 HIST1H1D 3007
Biochemical Activity Homo sapiens
95 RUNX1  
Biochemical Activity Homo sapiens
96 RB1 5925
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
97 PPM1B 5495
Co-fractionation Homo sapiens
98 SPAG5 10615
Biochemical Activity Homo sapiens
99 NF2 4771
Affinity Capture-MS Homo sapiens
100 SPDYE7P  
Affinity Capture-MS Homo sapiens
101 PPP1CA 5499
Affinity Capture-Western Homo sapiens
102 FAM96A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 DLD 1738
Affinity Capture-MS Homo sapiens
104 LMNB1 4001
Biochemical Activity Homo sapiens
105 TCP1 6950
Affinity Capture-MS Homo sapiens
106 FKBP5 2289
Affinity Capture-MS Homo sapiens
107 C19orf52  
Affinity Capture-MS Homo sapiens
108 COX15 1355
Affinity Capture-MS Homo sapiens
109 PRPH 5630
Proximity Label-MS Homo sapiens
110 SPDYC  
Affinity Capture-MS Homo sapiens
111 LZTS1  
Affinity Capture-Western Homo sapiens
112 CDKN1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
113 PARK2  
Affinity Capture-MS Homo sapiens
114 ZBTB16  
Affinity Capture-Western Homo sapiens
115 CCNB1IP1  
Biochemical Activity Homo sapiens
116 CUL3 8452
Affinity Capture-MS Homo sapiens
117 HIST1H1B 3009
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
118 LYN 4067
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
119 STAU1 6780
Affinity Capture-MS Homo sapiens
120 NCAPD2 9918
Biochemical Activity Homo sapiens
121 CD8A 925
Two-hybrid Homo sapiens
122 CIAO1 9391
Affinity Capture-MS Homo sapiens
123 UBE2I 7329
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
124 HIF1AN 55662
Affinity Capture-MS Homo sapiens
125 CCNB1 891
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
126 MAPT  
Biochemical Activity Homo sapiens
127 MKRN2 23609
Affinity Capture-RNA Homo sapiens
128 FYN 2534
Affinity Capture-Western Homo sapiens
129 TIMELESS  
Affinity Capture-Western Homo sapiens
130 FLG2 388698
Affinity Capture-MS Homo sapiens
131 VCP 7415
Affinity Capture-MS Homo sapiens
132 NTRK1 4914
Affinity Capture-MS Homo sapiens
133 GFAP 2670
Affinity Capture-MS Homo sapiens
134 SAR1B 51128
Affinity Capture-MS Homo sapiens
135 CDKN3 1033
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
136 CHEK1  
Affinity Capture-MS Homo sapiens
137 PARP1 142
Co-fractionation Homo sapiens
138 CXCR1  
Two-hybrid Homo sapiens
139 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 RPA4  
Proximity Label-MS Homo sapiens
141 EZH2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
142 SPDYE4  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
143 ANAPC4 29945
Affinity Capture-Western Homo sapiens
144 HECTD1 25831
Affinity Capture-MS Homo sapiens
145 FANCG 2189
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
146 POLE  
Co-fractionation Homo sapiens
147 BOLA2B 654483
Affinity Capture-MS Homo sapiens
148 TIA1 7072
Co-fractionation Homo sapiens
149 HDAC5 10014
Affinity Capture-MS Homo sapiens
150 CAPZB 832
Affinity Capture-MS Homo sapiens
151 DNM1L 10059
Affinity Capture-MS Homo sapiens
152 HIST1H2AB 8335
Biochemical Activity Homo sapiens
153 FBXO5  
Biochemical Activity Homo sapiens
154 ARID4A  
Biochemical Activity Homo sapiens
155 USP32 84669
Affinity Capture-MS Homo sapiens
156 MGMT 4255
Affinity Capture-Western Homo sapiens
157 CCNO  
Affinity Capture-MS Homo sapiens
158 CDK17 5128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 BAG3 9531
Affinity Capture-MS Homo sapiens
160 PKMYT1  
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 C9orf72  
Affinity Capture-MS Homo sapiens
162 ASCC3 10973
Affinity Capture-MS Homo sapiens
163 FBL 2091
Affinity Capture-MS Homo sapiens
164 CCNA2 890
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
165 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
166 SKP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 KCND3 3752
Affinity Capture-Western Homo sapiens
168 TP53BP1 7158
Biochemical Activity Homo sapiens
169 OTUD1 220213
Affinity Capture-MS Homo sapiens
170 CCNE2  
Affinity Capture-MS Homo sapiens
171 SDHA 6389
Affinity Capture-MS Homo sapiens
172 CSN2 1447
Biochemical Activity Homo sapiens
173 SIRT7  
Affinity Capture-MS Homo sapiens
174 CCP110  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
175 BARD1 580
Biochemical Activity Homo sapiens
176 TSPYL2  
Biochemical Activity Homo sapiens
177 SCYL2 55681
Affinity Capture-MS Homo sapiens
178 PRKAR2A 5576
Reconstituted Complex Homo sapiens
179 GADD45B  
Reconstituted Complex Homo sapiens
180 PAK6 56924
Two-hybrid Homo sapiens
181 CCNE1  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
182 UHRF2  
Far Western Homo sapiens
183 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
184 GLI1  
Affinity Capture-MS Homo sapiens
185 ARF6 382
Proximity Label-MS Homo sapiens
186 FBXO6 26270
Affinity Capture-MS Homo sapiens
187 KIAA1524 57650
Two-hybrid Homo sapiens
188 MYC  
Affinity Capture-MS Homo sapiens
189 CKS1B  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
190 HSD17B10 3028
Affinity Capture-MS Homo sapiens
191 WEE1 7465
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
192 FN1 2335
Affinity Capture-MS Homo sapiens
193 Anapc16  
Affinity Capture-MS Mus musculus
194 Espl1  
Affinity Capture-MS Mus musculus
195 JUP 3728
Affinity Capture-MS Homo sapiens
196 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
197 FANCA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
198 CIITA  
Biochemical Activity Homo sapiens
199 Cep72  
Affinity Capture-MS Mus musculus
200 PCBP1 5093
Co-fractionation Homo sapiens
201 DAB2 1601
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
202 ID2  
Biochemical Activity Homo sapiens
203 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
204 BAG1 573
Affinity Capture-MS Homo sapiens
205 ESCO1  
Affinity Capture-MS Homo sapiens
206 USP16  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
207 CCT4 10575
Affinity Capture-MS Homo sapiens
208 DLST 1743
Affinity Capture-MS Homo sapiens
209 EPN1 29924
Biochemical Activity Homo sapiens
210 DCD 117159
Affinity Capture-MS Homo sapiens
211 TOP1 7150
Co-fractionation Homo sapiens
212 USP19 10869
Affinity Capture-MS Homo sapiens
213 KAT5  
Biochemical Activity Homo sapiens
214 SIRT6  
Affinity Capture-MS Homo sapiens
215 HIST1H1A 3024
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
216 MOB2 81532
Affinity Capture-MS Homo sapiens
217 CCNA1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
218 KPRP 448834
Affinity Capture-MS Homo sapiens
219 DDRGK1 65992
Affinity Capture-MS Homo sapiens
220 RNF2  
Affinity Capture-MS Homo sapiens
221 VDAC1 7416
Affinity Capture-MS Homo sapiens
222 REPS2  
Biochemical Activity Homo sapiens
223 CDKN1B 1027
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
224 CCND1 595
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
225 PML 5371
Biochemical Activity Homo sapiens
226 MVD 4597
Affinity Capture-MS Homo sapiens
227 EEF2K  
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
228 HMGB1 3146
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
229 IKZF5 64376
Affinity Capture-MS Homo sapiens
230 CCDC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
231 NCAPG 64151
Biochemical Activity Homo sapiens
232 AR 367
Affinity Capture-MS Homo sapiens
233 DSP 1832
Affinity Capture-MS Homo sapiens
234 HIF1A 3091
Affinity Capture-MS Homo sapiens
235 MLKL 197259
Two-hybrid Homo sapiens
236 CUTA 51596
Affinity Capture-MS Homo sapiens
237 RNF43  
Proximity Label-MS Homo sapiens
238 TP53 7157
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
239 SNW1 22938
Affinity Capture-MS Homo sapiens
240 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
241 ICK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
242 CDC20 991
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
243 CCND2  
Affinity Capture-MS Homo sapiens
244 LAMTOR1 55004
Proximity Label-MS Homo sapiens
245 CCT7 10574
Affinity Capture-MS Homo sapiens
246 KIF26B  
Biochemical Activity Homo sapiens
247 HDGF 3068
Affinity Capture-MS Homo sapiens
248 ZRANB2 9406
Co-fractionation Homo sapiens
249 HIST1H2BB 3018
Biochemical Activity Homo sapiens
250 FANCC 2176
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
251 Hmga2  
Biochemical Activity Mus musculus
252 ERH 2079
Affinity Capture-MS Homo sapiens
253 XRCC6 2547
Biochemical Activity Homo sapiens
254 ZNF746  
Affinity Capture-MS Homo sapiens
255 HDAC6 10013
Affinity Capture-Western Homo sapiens
256 MISP 126353
Biochemical Activity Homo sapiens
257 KMT2E  
Biochemical Activity Homo sapiens
258 RPA1 6117
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
259 CDC37 11140
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
260 ACAA2 10449
Affinity Capture-MS Homo sapiens
261 RYK 6259
Affinity Capture-MS Homo sapiens
262 LCK 3932
Proximity Label-MS Homo sapiens
263 APLP2 334
Biochemical Activity Homo sapiens
264 FLNA 2316
Biochemical Activity Homo sapiens
265 SQSTM1 8878
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
266 ABL1 25
Biochemical Activity Homo sapiens
267 CDC6  
Biochemical Activity Homo sapiens
268 HSPB1 3315
Co-fractionation Homo sapiens
269 WAC  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
270 CDC25C  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
271 TSC1 7248
Reconstituted Complex Homo sapiens
272 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
273 CDK6 1021
Co-fractionation Homo sapiens
274 SIRT1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
275 MKI67  
Biochemical Activity Homo sapiens
276 Mprip  
Affinity Capture-MS Mus musculus
277 CDK1 983
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
278 LATS1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
279 TNNC1  
Two-hybrid Homo sapiens
280 ESR1  
Affinity Capture-MS Homo sapiens
281 UFL1 23376
Affinity Capture-MS Homo sapiens
282 KIF11 3832
Biochemical Activity Homo sapiens
283 JAK3 3718
Two-hybrid Homo sapiens
284 NCAPH 23397
Biochemical Activity Homo sapiens
285 TXN 7295
Affinity Capture-MS Homo sapiens
286 CEBPA  
Protein-peptide Homo sapiens
287 CDK13 8621
Affinity Capture-MS Homo sapiens
288 NCL 4691
Biochemical Activity Homo sapiens
289 BCL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
290 PSMA5 5686
Affinity Capture-MS Homo sapiens
291 CDK4 1019
Co-fractionation Homo sapiens
292 SORT1 6272
Affinity Capture-MS Homo sapiens
293 Samhd1 56045
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
294 RELB  
Two-hybrid Homo sapiens
295 HIST1H4A 8359
Biochemical Activity Homo sapiens
296 FAM96B 51647
Affinity Capture-MS Homo sapiens
297 ERCC6L 54821
Biochemical Activity Homo sapiens
298 FOXM1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
299 SERBP1 26135
Affinity Capture-MS Homo sapiens
300 CUL7 9820
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
301 KRAS 3845
Synthetic Lethality Homo sapiens
Proximity Label-MS Homo sapiens
Negative Genetic Homo sapiens
302 SLBP  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
303 CKS2  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
304 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
305 PRKCB 5579
Affinity Capture-Western Homo sapiens
306 MIS18BP1  
Biochemical Activity Homo sapiens
307 RPA2 6118
Co-fractionation Homo sapiens
308 PHF8  
Affinity Capture-MS Homo sapiens
309 RPA3 6119
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
310 SLC25A5 292
Affinity Capture-MS Homo sapiens
311 EP300 2033
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
312 MSI2 124540
Affinity Capture-MS Homo sapiens
313 POLD1 5424
Co-fractionation Homo sapiens
314 CCND3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CDK1 is involved
PathwayEvidenceSource
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Activation of NIMA Kinases NEK9, NEK6, NEK7 TAS Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint TAS Reactome
AURKA Activation by TPX2 TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation TAS Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex TAS Reactome
Cilium Assembly TAS Reactome
Condensation of Prometaphase Chromosomes IEA Reactome
Condensation of Prophase Chromosomes TAS Reactome
Cyclin A/B1/B2 associated events during G2/M transition TAS Reactome
Cyclin A/B1/B2 associated events during G2/M transition IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
Depolymerization of the Nuclear Lamina TAS Reactome
Deubiquitination TAS Reactome
E2F mediated regulation of DNA replication IEA Reactome
E2F-enabled inhibition of pre-replication complex formation IEA Reactome
G0 and Early G1 IEA Reactome
G1/S Transition IEA Reactome
G1/S-Specific Transcription IEA Reactome
G2/M Checkpoints TAS Reactome
G2/M DNA damage checkpoint TAS Reactome
G2/M DNA replication checkpoint TAS Reactome
G2/M Transition TAS Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome
Immune System TAS Reactome
Initiation of Nuclear Envelope (NE) Reformation TAS Reactome
Interferon Signaling TAS Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
MAPK3 (ERK1) activation TAS Reactome
MAPK6/MAPK4 signaling TAS Reactome
MASTL Facilitates Mitotic Progression IEA Reactome
Metabolism of proteins TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Prophase TAS Reactome
Mitotic Prophase IEA Reactome
Nuclear Envelope (NE) Reassembly TAS Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Organelle biogenesis and maintenance TAS Reactome
Ovarian tumor domain proteases TAS Reactome
Phosphorylation of Emi1 TAS Reactome
Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes TAS Reactome
Phosphorylation of the APC/C TAS Reactome
PKR-mediated signaling TAS Reactome
Post-translational protein modification TAS Reactome
RAF-independent MAPK1/3 activation TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of mitotic cell cycle TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Degradation TAS Reactome
Regulation of TP53 Expression and Degradation TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Signal Transduction TAS Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint TAS Reactome
TP53 Regulates Transcription of Cell Cycle Genes TAS Reactome
TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest TAS Reactome
Transcription of E2F targets under negative control by p107 (RBL1) and p130 (RBL2) in complex with HDAC1 IEA Reactome
Transcriptional regulation by RUNX2 TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome





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