Gene description for CDK1
Gene name cyclin-dependent kinase 1
Gene symbol CDK1
Other names/aliases CDC2
CDC28A
P34CDC2
Species Homo sapiens
 Database cross references - CDK1
ExoCarta ExoCarta_983
Entrez Gene 983
HGNC 1722
MIM 116940
UniProt P06493  
 CDK1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for CDK1
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    RNA polymerase II carboxy-terminal domain kinase activity GO:0008353 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 TAS
    Hsp70 protein binding GO:0030544 IEA
    ATP binding GO:0005524 IEA
    cyclin binding GO:0030332 IEA
    histone kinase activity GO:0035173 IEA
    protein binding GO:0005515 IPI
Biological Process
    innate immune response GO:0045087 TAS
    mitotic nuclear division GO:0007067 IEA
    protein localization to kinetochore GO:0034501 IDA
    positive regulation of protein import into nucleus, translocation GO:0033160 IEA
    peptidyl-threonine phosphorylation GO:0018107 IDA
    response to activity GO:0014823 IEA
    regulation of embryonic development GO:0045995 TAS
    DNA replication GO:0006260 TAS
    positive regulation of DNA replication GO:0045740 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    mitotic nuclear envelope disassembly GO:0007077 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    positive regulation of gene expression GO:0010628 IEA
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    ventricular cardiac muscle cell development GO:0055015 IEA
    activation of MAPK activity GO:0000187 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    response to axon injury GO:0048678 IEA
    epithelial cell differentiation GO:0030855 IEP
    insulin receptor signaling pathway GO:0008286 TAS
    response to cadmium ion GO:0046686 IEA
    response to ethanol GO:0045471 IEA
    regulation of Schwann cell differentiation GO:0014038 TAS
    response to toxic substance GO:0009636 IEA
    pronuclear fusion GO:0007344 TAS
    mitotic G2 DNA damage checkpoint GO:0007095 IEA
    organelle organization GO:0006996 TAS
    chromosome condensation GO:0030261 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    mitotic cell cycle GO:0000278 TAS
    histone phosphorylation GO:0016572 IEA
    apoptotic process GO:0006915 IEA
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    organ regeneration GO:0031100 IEA
    protein complex assembly GO:0006461 IEA
    response to copper ion GO:0046688 IEA
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    response to amine GO:0014075 IEA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    negative regulation of apoptotic process GO:0043066 IDA
    response to organic cyclic compound GO:0014070 IEA
    cell migration GO:0016477 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    axon guidance GO:0007411 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    peptidyl-serine phosphorylation GO:0018105 IDA
    positive regulation of mitotic cell cycle GO:0045931 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    positive regulation of cardiac muscle cell proliferation GO:0060045 IEA
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    microtubule cytoskeleton organization GO:0000226 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    cell aging GO:0007569 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    Ras protein signal transduction GO:0007265 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    activation of MAPKK activity GO:0000186 TAS
    cell division GO:0051301 IEA
    MAPK cascade GO:0000165 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    centrosome cycle GO:0007098 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0000083 TAS
    response to drug GO:0042493 IEA
    DNA repair GO:0006281 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    cellular response to hydrogen peroxide GO:0070301 IEA
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 IDA
    mitochondrion GO:0005739 TAS
    centrosome GO:0005813 IDA
    nucleoplasm GO:0005654 TAS
    mitotic spindle GO:0072686 IDA
    midbody GO:0030496 IDA
    spindle microtubule GO:0005876 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified CDK1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CDK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT7 29883
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 SFN 2810
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 LATS1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 NSFL1C 55968
Biochemical Activity Homo sapiens
5 PKMYT1  
Invitro Homo sapiens
6 FANCG  
Reconstituted Complex Homo sapiens
7 CCNA2 890
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
8 CCNB2  
Invivo Homo sapiens
Invitro Homo sapiens
9 TLE1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 FANCC 2176
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 PTMA 5757
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 LMNB1 4001
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
13 FEN1 2237
Reconstituted Complex Homo sapiens
14 ERCC2 2068
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
15 GADD45G  
Affinity Capture-MS Homo sapiens
16 GADD45A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 CDKN3 1033
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 TSC1  
Reconstituted Complex Homo sapiens
19 CUTL1  
Biochemical Activity Homo sapiens
20 BRCA1 672
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
21 PIN1 5300
Affinity Capture-MS Homo sapiens
22 CCNE1  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
23 USP16  
Affinity Capture-MS Homo sapiens
24 LZTS1  
Invivo Homo sapiens
25 ZBTB16  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
26 GORASP1  
Affinity Capture-MS Homo sapiens
27 CKS1B  
Affinity Capture-MS Homo sapiens
28 MKI67  
Biochemical Activity Homo sapiens
29 LYN 4067
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 PCNA 5111
Invitro Homo sapiens
31 CDC25C  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
32 FANCA  
Affinity Capture-MS Homo sapiens
33 CCNB1 891
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 BCL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 FYN 2534
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
36 SPAG5 10615
Biochemical Activity Homo sapiens
37 EEF1D 1936
Affinity Capture-MS Homo sapiens
38 TP53  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
39 CCNA1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 DAB2 1601
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
41 CKS2  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
42 PITPNM1 9600
Affinity Capture-MS Homo sapiens
43 CHAF1B  
Affinity Capture-MS Homo sapiens
44 BIRC5  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CDK1 is involved
PathwayEvidenceSource
Activation of NIMA Kinases NEK9, NEK6, NEK7 TAS Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex TAS Reactome
Condensation of Prometaphase Chromosomes TAS Reactome
Condensation of Prophase Chromosomes TAS Reactome
Cyclin A/B1 associated events during G2/M transition TAS Reactome
Cyclin B2 mediated events TAS Reactome
Depolymerisation of the Nuclear Lamina TAS Reactome
E2F mediated regulation of DNA replication TAS Reactome
E2F-enabled inhibition of pre-replication complex formation TAS Reactome
ERK1 activation TAS Reactome
ERK1 activation TAS Reactome
G0 and Early G1 TAS Reactome
G1/S-Specific Transcription TAS Reactome
G2/M DNA replication checkpoint TAS Reactome
Golgi Cisternae Pericentriolar Stack Reorganization TAS Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
MASTL Facilitates Mitotic Progression TAS Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Phosphorylation of Emi1 TAS Reactome
Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes TAS Reactome
Phosphorylation of the APC/C TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here