Gene description for MSI2
Gene name musashi RNA-binding protein 2
Gene symbol MSI2
Other names/aliases MSI2H
Species Homo sapiens
 Database cross references - MSI2
ExoCarta ExoCarta_124540
Vesiclepedia VP_124540
Entrez Gene 124540
HGNC 18585
MIM 607897
UniProt Q96DH6  
 MSI2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MSI2
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    poly(U) RNA binding GO:0008266 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    regulation of translation GO:0006417 IBA
    central nervous system development GO:0007417 IBA
    stem cell development GO:0048864 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified MSI2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MSI2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHMP2A 27243
Co-fractionation Homo sapiens
2 VCAM1 7412
Affinity Capture-MS Homo sapiens
3 MAGEA8  
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
6 EIF3G 8666
Co-fractionation Homo sapiens
7 CUL1 8454
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 BTRC 8945
Affinity Capture-Western Homo sapiens
9 MYCN  
Affinity Capture-MS Homo sapiens
10 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HNRNPH2 3188
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 CA10  
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 RBPMS 11030
Two-hybrid Homo sapiens
15 GRB2 2885
Two-hybrid Homo sapiens
16 Rbm38  
Two-hybrid Mus musculus
17 TRIM31  
Affinity Capture-MS Homo sapiens
18 TP53 7157
Affinity Capture-Western Homo sapiens
19 ANKRD40 91369
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 WDR1 9948
Co-fractionation Homo sapiens
21 RC3H2  
Affinity Capture-MS Homo sapiens
22 SRPK2 6733
Two-hybrid Homo sapiens
23 CPEB1  
Affinity Capture-MS Homo sapiens
24 TARDBP 23435
Affinity Capture-MS Homo sapiens
25 PIN1 5300
Co-fractionation Homo sapiens
26 RPA2 6118
Affinity Capture-MS Homo sapiens
27 UBE2L3 7332
Co-fractionation Homo sapiens
28 RPA1 6117
Affinity Capture-MS Homo sapiens
29 MRPL18 29074
Affinity Capture-MS Homo sapiens
30 DMRTB1  
Two-hybrid Homo sapiens
31 FOXA1  
Affinity Capture-MS Homo sapiens
32 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KIF20A 10112
Affinity Capture-MS Homo sapiens
34 LOXL3 84695
Affinity Capture-MS Homo sapiens
35 RHOBTB2  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 KHDRBS2  
Two-hybrid Homo sapiens
37 HNRNPA1 3178
Co-fractionation Homo sapiens
38 FKBP1A 2280
Co-fractionation Homo sapiens
39 SUZ12  
Affinity Capture-MS Homo sapiens
40 CDK1 983
Affinity Capture-MS Homo sapiens
41 MSI1  
Affinity Capture-MS Homo sapiens
42 ICK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ESR1  
Affinity Capture-MS Homo sapiens
44 EIF3E 3646
Two-hybrid Homo sapiens
45 ANXA13 312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RBFOX2 23543
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
47 PABPC1 26986
Co-fractionation Homo sapiens
48 Pcbp1 23983
Two-hybrid Mus musculus
49 RPA3 6119
Affinity Capture-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 UPF1 5976
Affinity Capture-MS Homo sapiens
52 PHYHIP 9796
Affinity Capture-MS Homo sapiens
53 RAB7A 7879
Co-fractionation Homo sapiens
54 TIMM10B 26515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 GRSF1 2926
Proximity Label-MS Homo sapiens
56 MEOX2  
Two-hybrid Homo sapiens
57 GJB1 2705
Affinity Capture-MS Homo sapiens
58 KIF21A 55605
Affinity Capture-MS Homo sapiens
59 RNF2  
Affinity Capture-MS Homo sapiens
60 BMI1  
Affinity Capture-MS Homo sapiens
61 QKI 9444
Two-hybrid Homo sapiens
62 RC3H1 149041
Affinity Capture-MS Homo sapiens
63 MBNL2 10150
Two-hybrid Homo sapiens
64 CTSB 1508
Co-fractionation Homo sapiens
65 STIP1 10963
Co-fractionation Homo sapiens
66 MDM2  
Affinity Capture-Western Homo sapiens
67 PABPC4 8761
Co-fractionation Homo sapiens
68 UFM1 51569
Co-fractionation Homo sapiens
69 USP10 9100
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
70 CRIP1 1396
Co-fractionation Homo sapiens
71 H2AFX 3014
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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