Gene description for Pcbp1
Gene name poly(rC) binding protein 1
Gene symbol Pcbp1
Other names/aliases WBP17
[a]CP-1
alphaCP-1
Species Mus musculus
 Database cross references - Pcbp1
ExoCarta ExoCarta_23983
Vesiclepedia VP_23983
Entrez Gene 23983
UniProt P60335  
 Pcbp1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pcbp1
Molecular Function
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IDA
    single-stranded DNA binding GO:0003697 IBA
    single-stranded DNA binding GO:0003697 IDA
    single-stranded DNA binding GO:0003697 ISO
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 TAS
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 ISO
    mRNA 3'-UTR binding GO:0003730 ISS
    protein binding GO:0005515 IPI
    translation activator activity GO:0008494 TAS
    iron chaperone activity GO:0034986 ISO
    sequence-specific single stranded DNA binding GO:0098847 IDA
Biological Process
    mRNA processing GO:0006397 TAS
    viral RNA genome replication GO:0039694 IEA
    viral RNA genome replication GO:0039694 ISO
    positive regulation of translation GO:0045727 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    protein maturation GO:0051604 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IMP
    cytosol GO:0005829 ISO
    postsynaptic density GO:0014069 IEA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified Pcbp1 in exosomes
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pcbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBPMS 11030
Two-hybrid Homo sapiens
2 Igf2bp2 319765
Two-hybrid Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 TNFAIP3 7128
Two-hybrid Homo sapiens
5 Rbm38  
Two-hybrid Mus musculus
6 DDX50 79009
Two-hybrid Homo sapiens
7 Rac1 19353
Co-fractionation Mus musculus
8 MSI2 124540
Two-hybrid Homo sapiens
9 Sarnp 66118
Co-fractionation Mus musculus
10 Stat3 20848
Affinity Capture-Western Mus musculus
Phenotypic Enhancement Mus musculus
11 RBFOX2 23543
Two-hybrid Homo sapiens
12 Stau1  
Co-fractionation Mus musculus
13 WDR3  
Two-hybrid Homo sapiens
14 Fubp3  
Two-hybrid Mus musculus
15 CTBP1 1487
Two-hybrid Homo sapiens
16 MRPL10 124995
Two-hybrid Homo sapiens
17 Rps4x 20102
Co-fractionation Mus musculus
18 ABCG1  
Affinity Capture-MS Homo sapiens
19 Rbm47  
Two-hybrid Mus musculus
20 DMRTB1  
Two-hybrid Homo sapiens
21 RBMX 27316
Two-hybrid Homo sapiens
22 USP10 9100
Two-hybrid Homo sapiens
23 QKI 9444
Two-hybrid Homo sapiens
24 Fmr1  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
25 Rbm39 170791
Co-fractionation Mus musculus
26 SNRPA 6626
Two-hybrid Homo sapiens
27 PTBP1 5725
Two-hybrid Homo sapiens
28 Tfe3  
Affinity Capture-MS Mus musculus
29 Rpl24 68193
Two-hybrid Mus musculus
30 PTBP3 9991
Two-hybrid Homo sapiens
31 Rps6 20104
Co-fractionation Mus musculus
32 Ncl 17975
Co-fractionation Mus musculus
33 Iqcb1  
Affinity Capture-MS Mus musculus
34 PTBP2  
Two-hybrid Homo sapiens
35 PRRC2B  
Two-hybrid Homo sapiens
36 YTHDF3  
Two-hybrid Homo sapiens
37 ZC3H10  
Two-hybrid Homo sapiens
38 Mettl21e  
Affinity Capture-MS Mus musculus
39 Tmem173  
Proximity Label-MS Mus musculus
40 PNO1 56902
Two-hybrid Homo sapiens
41 CPEB2  
Two-hybrid Homo sapiens
42 KHDRBS2  
Two-hybrid Homo sapiens
43 PRR3  
Two-hybrid Homo sapiens
44 HNRNPR 10236
Two-hybrid Homo sapiens
45 Gtf2f1  
Two-hybrid Mus musculus
46 Rock2  
Co-fractionation Mus musculus
47 Dynll1 56455
Two-hybrid Mus musculus
48 RPS13 6207
Two-hybrid Homo sapiens
49 RBM3 5935
Two-hybrid Homo sapiens
50 Unk  
Affinity Capture-RNA Mus musculus
51 RALYL 138046
Two-hybrid Homo sapiens
52 Rplp0 11837
Co-fractionation Mus musculus
53 CISD2 493856
Two-hybrid Homo sapiens
54 PCBP3 54039
Two-hybrid Homo sapiens
55 Hnrnpk 15387
Two-hybrid Mus musculus
56 ZC3HAV1 56829
Two-hybrid Homo sapiens
57 Eed  
Affinity Capture-MS Mus musculus
58 Il6  
Protein-RNA Mus musculus
59 Srsf3 20383
Two-hybrid Mus musculus
60 Mapt  
Affinity Capture-MS Mus musculus
61 LIN28A  
Two-hybrid Homo sapiens
62 Tia1  
Affinity Capture-MS Mus musculus
63 APOBEC3C 27350
Two-hybrid Homo sapiens
64 SRSF12  
Two-hybrid Homo sapiens
65 Otx2  
Affinity Capture-MS Mus musculus
66 Celf1  
Two-hybrid Mus musculus
67 Fancd2  
Affinity Capture-MS Mus musculus
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