Gene description for Srsf3
Gene name serine/arginine-rich splicing factor 3
Gene symbol Srsf3
Other names/aliases AL024116
Sfrs3
X16
Species Mus musculus
 Database cross references - Srsf3
ExoCarta ExoCarta_20383
Vesiclepedia VP_20383
Entrez Gene 20383
UniProt P84104  
 Srsf3 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Srsf3
Molecular Function
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    RNA binding GO:0003723 TAS
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    phospholipase binding GO:0043274 IPI
    primary miRNA binding GO:0070878 IEA
    primary miRNA binding GO:0070878 ISO
    protein-RNA sequence-specific adaptor activity GO:0160134 ISO
    protein-RNA sequence-specific adaptor activity GO:0160134 ISS
    sequence-specific mRNA binding GO:1990825 ISO
Biological Process
    mRNA processing GO:0006397 IEA
    mRNA export from nucleus GO:0006406 ISO
    mRNA export from nucleus GO:0006406 ISS
    insulin receptor signaling pathway GO:0008286 TAS
    RNA splicing GO:0008380 TAS
    primary miRNA processing GO:0031053 IEA
    primary miRNA processing GO:0031053 ISO
    mRNA cis splicing, via spliceosome GO:0045292 IBA
    regulation of mRNA splicing, via spliceosome GO:0048024 ISO
    regulation of mRNA splicing, via spliceosome GO:0048024 ISS
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    cytoplasm GO:0005737 IEA
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    nuclear speck GO:0016607 ISO
 Experiment description of studies that identified Srsf3 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Srsf3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tfip11  
Co-fractionation Mus musculus
2 Cpeb4  
Two-hybrid Mus musculus
3 1110037F02Rik 66185
Co-fractionation Mus musculus
4 Prpf4b 19134
Co-fractionation Mus musculus
5 PCBP3 54039
Two-hybrid Homo sapiens
6 Phb2 12034
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
7 Cluh  
Affinity Capture-MS Mus musculus
8 Taf15  
Two-hybrid Mus musculus
9 CLK1  
Two-hybrid Homo sapiens
10 Srrm1 51796
Co-fractionation Mus musculus
11 Fndc5  
Affinity Capture-MS Mus musculus
12 Plcb1  
Affinity Capture-Western Rattus norvegicus
13 Tra2b 20462
Two-hybrid Mus musculus
14 LSM2 57819
Two-hybrid Homo sapiens
15 Snrnp40  
Co-fractionation Mus musculus
16 RBMX 27316
Two-hybrid Homo sapiens
17 Bcas2  
Co-fractionation Mus musculus
18 Srsf7 225027
Co-fractionation Mus musculus
19 Lmb1  
Affinity Capture-Western Mus musculus
20 Atxn1  
Affinity Capture-MS Mus musculus
21 Cebpa  
Affinity Capture-MS Mus musculus
22 PTBP1 5725
Two-hybrid Homo sapiens
23 Htt  
Affinity Capture-MS Mus musculus
24 SNRPA 6626
Two-hybrid Homo sapiens
25 Eef1a2 13628
Affinity Capture-Western Mus musculus
26 Usp48  
Affinity Capture-MS Mus musculus
27 Pcbp1 23983
Two-hybrid Mus musculus
28 Slc6a4  
Affinity Capture-MS Mus musculus
29 Mov10 17454
Co-fractionation Mus musculus
30 NXF1 10482
Two-hybrid Homo sapiens
31 Cactin  
Co-fractionation Mus musculus
32 SRSF8 10929
Two-hybrid Homo sapiens
33 Thoc2  
Co-fractionation Mus musculus
34 Srsf1 110809
Co-fractionation Mus musculus
35 Slu7  
Co-fractionation Mus musculus
36 Srsf2 20382
Co-fractionation Mus musculus
37 RBM3 5935
Two-hybrid Homo sapiens
38 Cdk12  
Co-fractionation Mus musculus
39 Smu1  
Co-fractionation Mus musculus
40 Htatsf1  
Co-fractionation Mus musculus
41 Adcy10  
Co-fractionation Mus musculus
42 Tnpo3 320938
Co-fractionation Mus musculus
43 Hnrnpk 15387
Two-hybrid Mus musculus
44 Eed  
Affinity Capture-MS Mus musculus
45 Srsf3 20383
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
46 U2af2 22185
Two-hybrid Mus musculus
47 Mapt  
Affinity Capture-MS Mus musculus
48 Ik  
Co-fractionation Mus musculus
49 Cfd  
Affinity Capture-MS Mus musculus
50 Rbm25  
Co-fractionation Mus musculus
51 Fancd2  
Affinity Capture-MS Mus musculus
52 Kctd13  
Affinity Capture-MS Mus musculus
53 Spen  
Co-fractionation Mus musculus
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