Gene description for RBMX
Gene name RNA binding motif protein, X-linked
Gene symbol RBMX
Other names/aliases HNRNPG
HNRPG
RBMXP1
RBMXRT
RNMX
hnRNP-G
Species Homo sapiens
 Database cross references - RBMX
ExoCarta ExoCarta_27316
Entrez Gene 27316
HGNC 9910
MIM 300199
UniProt P38159  
 RBMX identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RBMX
Molecular Function
    nucleotide binding GO:0000166 IEA
    core promoter binding GO:0001047 IDA
    protein binding GO:0005515 IPI
    mRNA binding GO:0003729 IDA
    chromatin binding GO:0003682 IDA
    single-stranded RNA binding GO:0003727 IEA
    poly(A) RNA binding GO:0044822 IDA
    RNA binding GO:0003723 NAS
Biological Process
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISS
    RNA splicing GO:0008380 TAS
    gene expression GO:0010467 TAS
    osteoblast differentiation GO:0001649 IDA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IDA
    mRNA splicing, via spliceosome GO:0000398 TAS
    protein homooligomerization GO:0051260 ISS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISS
    transcription from RNA polymerase II promoter GO:0006366 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    mRNA splice site selection GO:0006376 IEA
    cellular response to interleukin-1 GO:0071347 IDA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    ribonucleoprotein complex GO:0030529 NAS
    extracellular space GO:0005615 IDA
    supraspliceosomal complex GO:0044530 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 IDA
    nuclear euchromatin GO:0005719 IDA
 Experiment description of studies that identified RBMX in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RBMX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBM7  
Two-hybrid Homo sapiens
2 HNRNPK 3190
Two-hybrid Homo sapiens
3 SORBS3 10174
Two-hybrid Homo sapiens
4 KHDRBS2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 KHDRBS3  
Two-hybrid Homo sapiens
6 ZFHX3  
Affinity Capture-MS Homo sapiens
7 LNX  
Two-hybrid Homo sapiens
8 FXR2 9513
Two-hybrid Homo sapiens
9 CIRBP 1153
Two-hybrid Homo sapiens
10 PSMF1  
Two-hybrid Homo sapiens
11 MGC2560  
Two-hybrid Homo sapiens
12 TRA2B 6434
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
13 CCDC33 80125
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RBMX is involved
PathwayEvidenceSource
mRNA Splicing - Major Pathway TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome





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