Gene description for JAK2
Gene name Janus kinase 2
Gene symbol JAK2
Other names/aliases JTK10
THCYT3
Species Homo sapiens
 Database cross references - JAK2
ExoCarta ExoCarta_3717
Vesiclepedia VP_3717
Entrez Gene 3717
HGNC 6192
MIM 147796
UniProt O60674  
 JAK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for JAK2
Molecular Function
    heme binding GO:0020037 IDA
    interleukin-12 receptor binding GO:0005143 ISS
    type 1 angiotensin receptor binding GO:0031702 IEA
    receptor binding GO:0005102 IPI
    protein tyrosine kinase activity GO:0004713 TAS
    histone kinase activity (H3-Y41 specific) GO:0035401 IDA
    histone binding GO:0042393 IDA
    ATP binding GO:0005524 IEA
    insulin receptor substrate binding GO:0043560 IEA
    growth hormone receptor binding GO:0005131 ISS
    protein kinase binding GO:0019901 IDA
    SH2 domain binding GO:0042169 IPI
    acetylcholine receptor binding GO:0033130 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    protein C-terminus binding GO:0008022 IEA
    protein kinase activity GO:0004672 NAS
    peptide hormone receptor binding GO:0051428 IEA
    phosphatidylinositol 3-kinase binding GO:0043548 IEA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of cell proliferation GO:0008284 IEA
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IEA
    host programmed cell death induced by symbiont GO:0034050 IEA
    activation of JAK2 kinase activity GO:0042977 ISS
    regulation of inflammatory response GO:0050727 IDA
    response to interleukin-12 GO:0070671 IDA
    movement of cell or subcellular component GO:0006928 TAS
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell differentiation GO:0045597 IEA
    positive regulation of tyrosine phosphorylation of Stat5 protein GO:0042523 ISS
    tyrosine phosphorylation of Stat5 protein GO:0042506 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    regulation of apoptotic process GO:0042981 IBA
    positive regulation of inflammatory response GO:0050729 IEA
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 ISS
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IEA
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    axon regeneration GO:0031103 IEA
    tyrosine phosphorylation of Stat1 protein GO:0042508 IEA
    regulation of cell proliferation GO:0042127 IBA
    tumor necrosis factor-mediated signaling pathway GO:0033209 IDA
    signal transduction GO:0007165 ISS
    mesoderm development GO:0007498 TAS
    activation of MAPKK activity GO:0000186 IEA
    response to tumor necrosis factor GO:0034612 IDA
    apoptotic process GO:0006915 ISS
    tyrosine phosphorylation of Stat3 protein GO:0042503 IEA
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    protein autophosphorylation GO:0046777 ISS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    cell differentiation GO:0030154 ISS
    positive regulation of insulin secretion GO:0032024 IEA
    negative regulation of cell-cell adhesion GO:0022408 IEA
    intracellular signal transduction GO:0035556 ISS
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 ISS
    negative regulation of cell proliferation GO:0008285 ISS
    JAK-STAT cascade GO:0007259 TAS
    negative regulation of neuron apoptotic process GO:0043524 IEA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    growth hormone receptor signaling pathway GO:0060396 IDA
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    negative regulation of heart contraction GO:0045822 IEA
    positive regulation of phosphoprotein phosphatase activity GO:0032516 IEA
    response to antibiotic GO:0046677 IDA
    negative regulation of DNA binding GO:0043392 ISS
    protein phosphorylation GO:0006468 TAS
    hormone-mediated signaling pathway GO:0009755 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 ISS
    mineralocorticoid receptor signaling pathway GO:0031959 IEA
    histone H3-Y41 phosphorylation GO:0035409 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 ISS
    extrinsic apoptotic signaling pathway GO:0097191 ISS
    positive regulation of DNA binding GO:0043388 IEA
    positive regulation of protein import into nucleus, translocation GO:0033160 IEA
    cell migration GO:0016477 IBA
    mammary gland epithelium development GO:0061180 ISS
    blood coagulation GO:0007596 TAS
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    positive regulation of interleukin-1 beta production GO:0032731 IEA
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    innate immune response GO:0045087 IBA
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    positive regulation of cell activation GO:0050867 IEA
    interleukin-12-mediated signaling pathway GO:0035722 IDA
    enzyme linked receptor protein signaling pathway GO:0007167 ISS
    erythrocyte differentiation GO:0030218 ISS
    chromatin organization GO:0006325 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 ISS
    response to lipopolysaccharide GO:0032496 ISS
    tyrosine phosphorylation of STAT protein GO:0007260 ISS
    positive regulation of tyrosine phosphorylation of Stat3 protein GO:0042517 ISS
    response to hydroperoxide GO:0033194 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISS
    STAT protein import into nucleus GO:0007262 ISS
    positive regulation of growth hormone receptor signaling pathway GO:0060399 ISS
    regulation of interferon-gamma-mediated signaling pathway GO:0060334 TAS
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    actin filament polymerization GO:0030041 NAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    endosome lumen GO:0031904 TAS
    nucleus GO:0005634 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    nuclear matrix GO:0016363 IEA
    cytoskeleton GO:0005856 IEA
    cytosol GO:0005829 TAS
    caveola GO:0005901 ISS
    membrane raft GO:0045121 ISS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified JAK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for JAK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCR2  
Affinity Capture-Western Homo sapiens
2 SOCS3 9021
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 PTPN11 5781
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Biochemical Activity Homo sapiens
4 PPP2R4 5524
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 ERBB2 2064
Affinity Capture-Western Homo sapiens
6 CXCR4 7852
Affinity Capture-Western Homo sapiens
7 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
9 STAT5B 6777
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
10 HSPA8 3312
Affinity Capture-Western Homo sapiens
11 PTPN6 5777
Affinity Capture-Western Homo sapiens
12 STAT5A 6776
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 APS  
Biochemical Activity Homo sapiens
14 TSHR 7253
Invivo Homo sapiens
15 STAM2 10254
Invivo Homo sapiens
16 BRCA1 672
Affinity Capture-Western Homo sapiens
17 TNFRSF1A 7132
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 DNAJA3 9093
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 SOCS1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 PPIA 5478
Affinity Capture-Western Homo sapiens
22 GRB10 2887
Invivo Homo sapiens
Invitro Homo sapiens
23 PTPN12  
Affinity Capture-Western Homo sapiens
24 PTK2 5747
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
25 PTPN1 5770
Invivo Homo sapiens
26 TUB  
Invitro Homo sapiens
27 IL5RA  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 ELP2 55250
Affinity Capture-Western Homo sapiens
29 LYN 4067
Reconstituted Complex Homo sapiens
30 HTR2A  
Affinity Capture-Western Homo sapiens
31 TEC 7006
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
32 LEPR 3953
Invitro Homo sapiens
33 PRMT5 10419
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
34 CSF2RB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
35 PPP2R1B 5519
Invivo Homo sapiens
36 FYN 2534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 CTLA4  
Invivo Homo sapiens
38 KIT 3815
Affinity Capture-Western Homo sapiens
39 SIRPA 140885
Affinity Capture-Western Homo sapiens
40 PTK2B 2185
Affinity Capture-Western Homo sapiens
41 SH2B1 25970
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
42 YES1 7525
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 RAF1 5894
Reconstituted Complex Homo sapiens
44 PRLR  
Invivo Homo sapiens
45 GHR  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
46 STAT3 6774
Invivo Homo sapiens
47 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 IL12RB2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
49 BCR 613
Invivo Homo sapiens
Invitro Homo sapiens
50 VAV1 7409
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
51 IRS1 3667
Biochemical Activity Homo sapiens
52 OSMR 9180
Invivo Homo sapiens
53 EPOR  
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
54 STAM 8027
Invivo Homo sapiens
55 VCP 7415
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here