Gene description for MAP3K5
Gene name mitogen-activated protein kinase kinase kinase 5
Gene symbol MAP3K5
Other names/aliases ASK1
MAPKKK5
MEKK5
Species Homo sapiens
 Database cross references - MAP3K5
ExoCarta ExoCarta_4217
Vesiclepedia VP_4217
Entrez Gene 4217
HGNC 6857
MIM 602448
UniProt Q99683  
 MAP3K5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP3K5
Molecular Function
    protein kinase binding GO:0019901 IEA
    magnesium ion binding GO:0000287 IDA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    protein kinase activity GO:0004672 IDA
    ATP binding GO:0005524 IDA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IDA
    MAP kinase kinase kinase activity GO:0004709 IDA
    protein phosphatase binding GO:0019903 IPI
Biological Process
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IDA
    activation of JUN kinase activity GO:0007257 TAS
    viral process GO:0016032 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
    JNK cascade GO:0007254 IDA
    innate immune response GO:0045087 IEA
    MAPK cascade GO:0000165 IDA
    response to ischemia GO:0002931 IEA
    apoptotic signaling pathway GO:0097190 TAS
    cellular response to hydrogen peroxide GO:0070301 IDA
    activation of MAPKK activity GO:0000186 IDA
    response to endoplasmic reticulum stress GO:0034976 IMP
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISS
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 IDA
    positive regulation of apoptotic process GO:0043065 IDA
    protein phosphorylation GO:0006468 IDA
    programmed necrotic cell death GO:0097300 IEA
    positive regulation of JNK cascade GO:0046330 ISS
    positive regulation of neuron death GO:1901216 ISS
    positive regulation of JUN kinase activity GO:0043507 IMP
    cellular response to reactive nitrogen species GO:1902170 IEA
Subcellular Localization
    IRE1-TRAF2-ASK1 complex GO:1990604 IDA
    protein kinase complex GO:1902911 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAP3K5 in exosomes
1
Experiment ID 489
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
ISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
ISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP3K5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K7 5609
Affinity Capture-Western Homo sapiens
2 DUSP19  
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
3 CDC25A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 MAP2K6 5608
Reconstituted Complex Homo sapiens
5 MAP3K6  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 ERN1  
Affinity Capture-Western Homo sapiens
7 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 QARS 5859
Affinity Capture-Western Homo sapiens
10 GADD45B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 GLRX 2745
Invitro Homo sapiens
12 EIF2AK2 5610
Invivo Homo sapiens
Invitro Homo sapiens
13 PDCD6 10016
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
14 EPS15 2060
Affinity Capture-Western Homo sapiens
15 PPP5C 5536
Invivo Homo sapiens
Two-hybrid Homo sapiens
16 YWHAZ 7534
Affinity Capture-Western Homo sapiens
17 CDKN1A  
Affinity Capture-Western Homo sapiens
18 HSPA1A 3303
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 MAP3K7  
Invivo Homo sapiens
Two-hybrid Homo sapiens
20 TRAF3 7187
Affinity Capture-Western Homo sapiens
21 RB1CC1 9821
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 Rb1cc1  
Affinity Capture-Western Mus musculus
23 MAP3K5 4217
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
24 TXN 7295
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 Gstm1 14862
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
26 TRAF5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 MAPK8IP3  
Invivo Homo sapiens
Invitro Homo sapiens
28 RAF1 5894
Invitro Homo sapiens
Invivo Homo sapiens
29 GSTM1 2944
Invivo Homo sapiens
Invitro Homo sapiens
30 DAXX  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
31 TRAF1 7185
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MAP3K5 is involved
PathwayEvidenceSource
Oxidative Stress Induced Senescence TAS Reactome





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