Gene description for TNFAIP3
Gene name tumor necrosis factor, alpha-induced protein 3
Gene symbol TNFAIP3
Other names/aliases A20
OTUD7C
TNFA1P2
Species Homo sapiens
 Database cross references - TNFAIP3
ExoCarta ExoCarta_7128
Vesiclepedia VP_7128
Entrez Gene 7128
HGNC 11896
MIM 191163
UniProt P21580  
 TNFAIP3 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TNFAIP3
Molecular Function
    DNA binding GO:0003677 IEA
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
    protein self-association GO:0043621 IDA
    ubiquitin-specific protease activity GO:0004843 TAS
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IEA
    ubiquitin binding GO:0043130 IPI
    ubiquitin-protein transferase activity GO:0004842 IDA
    protease binding GO:0002020 IPI
Biological Process
    inflammatory response GO:0006954 IEA
    negative regulation of chronic inflammatory response GO:0002677 IEA
    tolerance induction to lipopolysaccharide GO:0072573 IMP
    positive regulation of protein catabolic process GO:0045732 IDA
    negative regulation of interleukin-1 beta production GO:0032691 IEA
    cellular response to lipopolysaccharide GO:0071222 IDA
    B-1 B cell homeostasis GO:0001922 ISS
    negative regulation of inflammatory response GO:0050728 ISS
    regulation of defense response to virus by host GO:0050691 NAS
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IEA
    positive regulation of hepatocyte proliferation GO:2000347 IEA
    negative regulation of bone resorption GO:0045779 NAS
    innate immune response GO:0045087 TAS
    negative regulation of toll-like receptor 5 signaling pathway GO:0034148 IEA
    response to molecule of bacterial origin GO:0002237 IDA
    negative regulation of toll-like receptor 4 signaling pathway GO:0034144 NAS
    negative regulation of type I interferon production GO:0032480 TAS
    negative regulation of interleukin-6 production GO:0032715 ISS
    protein oligomerization GO:0051259 NAS
    apoptotic process GO:0006915 IEA
    protein K63-linked deubiquitination GO:0070536 IDA
    negative regulation of osteoclast proliferation GO:0090291 NAS
    protein K11-linked deubiquitination GO:0035871 IDA
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070433 IEA
    negative regulation of innate immune response GO:0045824 ISS
    negative regulation of toll-like receptor 2 signaling pathway GO:0034136 NAS
    negative regulation of B cell activation GO:0050869 ISS
    cellular response to hydrogen peroxide GO:0070301 ISS
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IDA
    protein K48-linked deubiquitination GO:0071108 IDA
    negative regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway GO:0070429 IEA
    negative regulation of CD40 signaling pathway GO:2000349 IMP
    negative regulation of interleukin-2 production GO:0032703 IMP
    regulation of germinal center formation GO:0002634 ISS
    negative regulation of endothelial cell apoptotic process GO:2000352 IDA
    negative regulation of protein ubiquitination GO:0031397 IDA
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    establishment of protein localization to vacuole GO:0072666 IEA
    protein K48-linked ubiquitination GO:0070936 IDA
    regulation of vascular wound healing GO:0061043 NAS
    protein deubiquitination GO:0016579 TAS
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    response to muramyl dipeptide GO:0032495 IEA
    negative regulation of toll-like receptor 3 signaling pathway GO:0034140 IDA
    negative regulation of tumor necrosis factor production GO:0032720 ISS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IDA
    positive regulation of cellular protein catabolic process GO:1903364 IEA
Subcellular Localization
    lysosome GO:0005764 IEA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
 Experiment description of studies that identified TNFAIP3 in exosomes
1
Experiment ID 79
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ EV Biophysical techniques
✔
CD81|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TNFAIP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 YWHAE 7531
Invivo Homo sapiens
3 TNFAIP3 7128
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 IKBKG 8517
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 TNIP1 10318
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 YWHAG 7532
Invivo Homo sapiens
Two-hybrid Homo sapiens
7 TRAF6 7189
Invivo Homo sapiens
8 YWHAZ 7534
Two-hybrid Homo sapiens
9 TRAF2 7186
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 IKBKB 3551
Invivo Homo sapiens
Invitro Homo sapiens
11 CHUK 1147
Invitro Homo sapiens
Invivo Homo sapiens
12 TAX1BP1 8887
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
13 YWHAB 7529
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
14 TRAF1 7185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TNFAIP3 is involved
PathwayEvidenceSource
Negative regulators of RIG-I/MDA5 signaling TAS Reactome
NOD1/2 Signaling Pathway TAS Reactome





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