Gene description for VRK1
Gene name vaccinia related kinase 1
Gene symbol VRK1
Other names/aliases PCH1
PCH1A
Species Homo sapiens
 Database cross references - VRK1
ExoCarta ExoCarta_7443
Entrez Gene 7443
HGNC 12718
MIM 602168
UniProt Q99986  
 VRK1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Thymus 23844026    
 Gene ontology annotations for VRK1
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein kinase binding GO:0019901 IDA
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    histone kinase activity (H3-T3 specific) GO:0072354 IDA
    histone kinase activity (H3-S10 specific) GO:0035175 IDA
    protein binding GO:0005515 IPI
    nucleosomal histone binding GO:0031493 IPI
Biological Process
    cell division GO:0051301 IEA
    Golgi disassembly GO:0090166 IDA
    mitotic nuclear envelope reassembly GO:0007084 TAS
    regulation of cell shape GO:0008360 IBA
    protein phosphorylation GO:0006468 IDA
    protein autophosphorylation GO:0046777 IDA
    endocytosis GO:0006897 IBA
    histone H3-S10 phosphorylation GO:0043987 IDA
    mitotic cell cycle GO:0000278 TAS
    histone H3-T3 phosphorylation GO:0072355 IDA
    mitotic nuclear division GO:0007067 IEA
    Wnt signaling pathway GO:0016055 IBA
    mitotic nuclear envelope disassembly GO:0007077 TAS
Subcellular Localization
    Golgi stack GO:0005795 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    spindle GO:0005819 IEA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified VRK1 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VRK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here