Gene description for RPL18A
Gene name ribosomal protein L18a
Gene symbol RPL18A
Other names/aliases L18A
Species Homo sapiens
 Database cross references - RPL18A
ExoCarta ExoCarta_6142
Entrez Gene 6142
HGNC 10311
MIM 604178
UniProt Q02543  
 RPL18A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RPL18A
Molecular Function
    structural constituent of ribosome GO:0003735 NAS
    poly(A) RNA binding GO:0044822 IDA
    RNA binding GO:0003723 TAS
Biological Process
    translational termination GO:0006415 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    viral life cycle GO:0019058 TAS
    translational elongation GO:0006414 TAS
    viral transcription GO:0019083 TAS
    translational initiation GO:0006413 TAS
    gene expression GO:0010467 TAS
    translation GO:0006412 TAS
    cellular protein metabolic process GO:0044267 TAS
    viral process GO:0016032 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytosolic large ribosomal subunit GO:0022625 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified RPL18A in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPL18A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RTCA 8634
Affinity Capture-MS Homo sapiens
2 RPL36 25873
Two-hybrid Homo sapiens
3 RIBC2  
Two-hybrid Homo sapiens
4 LYAR 55646
Affinity Capture-MS Homo sapiens
5 PSTPIP1 9051
Affinity Capture-MS Homo sapiens
6 LPL 4023
Two-hybrid Homo sapiens
7 DDX56  
Affinity Capture-MS Homo sapiens
8 MDK 4192
Two-hybrid Homo sapiens
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