Gene description for MDK
Gene name
midkine (neurite growth-promoting factor 2)
Gene symbol
MDK
Other names/aliases
ARAP MK NEGF2
Species
Homo sapiens
Database cross references - MDK
ExoCarta
ExoCarta_4192
Entrez Gene
4192
HGNC
6972
MIM
162096
UniProt
P21741
MDK identified in exosomes derived from the following tissue/cell type
Melanoma cells
25950383
Neuroblastoma cells
25944692
Gene ontology annotations for MDK
Experiment description of studies that identified MDK in exosomes
1
Experiment ID
258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
Protein-protein interactions for MDK
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
STAT1
6772
Affinity Capture-MS
Homo sapiens
2
NID1
4811
Affinity Capture-MS
Homo sapiens
3
NID2
22795
Affinity Capture-MS
Homo sapiens
4
SDC4
6385
Invitro
Homo sapiens
5
TUBA1A
7846
Affinity Capture-MS
Homo sapiens
6
NCAM1
4684
Affinity Capture-MS
Homo sapiens
7
TUBB2B
347733
Affinity Capture-MS
Homo sapiens
8
RPL18A
6142
Two-hybrid
Homo sapiens
9
SDC3
Affinity Capture-MS
Homo sapiens
10
NCL
4691
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
11
ACTG1
71
Affinity Capture-MS
Homo sapiens
12
SDC1
6382
Affinity Capture-MS
Homo sapiens
13
PTPRZ1
Invivo
Homo sapiens
Invitro
Homo sapiens
14
LRP1
4035
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which MDK is involved