Gene description for MDK
Gene name midkine (neurite growth-promoting factor 2)
Gene symbol MDK
Other names/aliases ARAP
MK
NEGF2
Species Homo sapiens
 Database cross references - MDK
ExoCarta ExoCarta_4192
Entrez Gene 4192
HGNC 6972
MIM 162096
UniProt P21741  
 MDK identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
 Gene ontology annotations for MDK
Molecular Function
    growth factor activity GO:0008083 NAS
    heparin binding GO:0008201 IDA
Biological Process
    short-term memory GO:0007614 IEA
    Notch signaling pathway GO:0007219 TAS
    behavioral fear response GO:0001662 IEA
    nervous system development GO:0007399 NAS
    response to glucocorticoid GO:0051384 IEA
    positive regulation of cell division GO:0051781 IEA
    defecation GO:0030421 IEA
    cell differentiation GO:0030154 NAS
    response to wounding GO:0009611 ISS
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    cell migration GO:0016477 IEA
    regulation of behavior GO:0050795 IEA
    cerebellar granular layer development GO:0021681 IEA
    cerebral cortex development GO:0021987 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    dentate gyrus development GO:0021542 IEA
    signal transduction GO:0007165 NAS
    adrenal gland development GO:0030325 ISS
    response to drug GO:0042493 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IEA
    cell projection GO:0042995 IEA
 Experiment description of studies that identified MDK in exosomes
1
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for MDK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STAT1 6772
Affinity Capture-MS Homo sapiens
2 NID1 4811
Affinity Capture-MS Homo sapiens
3 NID2 22795
Affinity Capture-MS Homo sapiens
4 SDC4 6385
Invitro Homo sapiens
5 TUBA1A 7846
Affinity Capture-MS Homo sapiens
6 NCAM1 4684
Affinity Capture-MS Homo sapiens
7 TUBB2B 347733
Affinity Capture-MS Homo sapiens
8 RPL18A 6142
Two-hybrid Homo sapiens
9 SDC3  
Affinity Capture-MS Homo sapiens
10 NCL 4691
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
11 ACTG1 71
Affinity Capture-MS Homo sapiens
12 SDC1 6382
Affinity Capture-MS Homo sapiens
13 PTPRZ1  
Invivo Homo sapiens
Invitro Homo sapiens
14 LRP1 4035
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MDK is involved
PathwayEvidenceSource
NOTCH2 Activation and Transmission of Signal to the Nucleus TAS Reactome





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