Gene description for NID2
Gene name nidogen 2 (osteonidogen)
Gene symbol NID2
Other names/aliases NID-2
Species Homo sapiens
 Database cross references - NID2
ExoCarta ExoCarta_22795
Entrez Gene 22795
HGNC 13389
MIM 605399
UniProt Q14112  
 NID2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NID2
Molecular Function
    collagen binding GO:0005518 TAS
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IEA
Biological Process
    extracellular matrix organization GO:0030198 TAS
    basement membrane organization GO:0071711 TAS
    cell-matrix adhesion GO:0007160 IEA
    cell adhesion GO:0007155 TAS
Subcellular Localization
    cell surface GO:0009986 IEA
    extracellular matrix GO:0031012 ISS
    extracellular region GO:0005576 TAS
    extracellular exosome GO:0070062 IDA
    basement membrane GO:0005604 IDA
 Experiment description of studies that identified NID2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NID2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL1A1 1277
Reconstituted Complex Homo sapiens
2 ELN  
Reconstituted Complex Homo sapiens
3 LAMC1 3915
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 PRELP 5549
Reconstituted Complex Homo sapiens
5 COL4A1 1282
Reconstituted Complex Homo sapiens
6 HSPG2 3339
Reconstituted Complex Homo sapiens
7 MDK 4192
Affinity Capture-MS Homo sapiens
8 COL13A1  
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which NID2 is involved
PathwayEvidenceSource
Laminin interactions TAS Reactome





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