Experiment description of studies that identified MATN2 in exosomes

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 282 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 283 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

5 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 250 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|TSG101|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25857301     Organism Homo sapiens Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles. Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D" Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - expressing DIAPH3 hRNA Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for MATN2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 A1BG 1 Affinity Capture-MS Homo sapiens 2 HBB 3043 Affinity Capture-MS Homo sapiens 3 HBD 3045 Affinity Capture-MS Homo sapiens 4 ITIH4 3700 Affinity Capture-MS Homo sapiens 5 FBN2 2201 Reconstituted Complex Homo sapiens 6 RPL30 6156 Affinity Capture-MS Homo sapiens 7 TTR 7276 Affinity Capture-MS Homo sapiens 8 ALB 213 Affinity Capture-MS Homo sapiens 9 ADSSL1   Affinity Capture-MS Homo sapiens 10 RPL10 6134 Affinity Capture-MS Homo sapiens 11 RPL14 9045 Affinity Capture-MS Homo sapiens 12 ITIH2 3698 Affinity Capture-MS Homo sapiens 13 DVL3 1857 Two-hybrid Homo sapiens 14 MATN1   Reconstituted Complex Homo sapiens 15 HPX 3263 Affinity Capture-MS Homo sapiens 16 GRHPR 9380 Affinity Capture-MS Homo sapiens 17 HP 3240 Affinity Capture-MS Homo sapiens 18 RPL28 6158 Affinity Capture-MS Homo sapiens 19 KIF23 9493 Affinity Capture-MS Homo sapiens 20 ORM1 5004 Affinity Capture-MS Homo sapiens 21 C3 718 Affinity Capture-MS Homo sapiens 22 RPL24 6152 Affinity Capture-MS Homo sapiens 23 NPM1 4869 Affinity Capture-MS Homo sapiens 24 SRRM2 23524 Affinity Capture-MS Homo sapiens 25 KHDRBS1 10657 Affinity Capture-MS Homo sapiens 26 NID2 22795 Affinity Capture-MS Homo sapiens 27 SERPINA1 5265 Affinity Capture-MS Homo sapiens 28 NELL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 PXDN 7837 Affinity Capture-MS Homo sapiens 30 VTN 7448 Affinity Capture-MS Homo sapiens 31 RPL27 6155 Affinity Capture-MS Homo sapiens 32 THBS4 7060 Affinity Capture-MS Homo sapiens 33 S100A2 6273 Affinity Capture-MS Homo sapiens 34 CMAS 55907 Affinity Capture-MS Homo sapiens 35 IGHA2 3494 Affinity Capture-MS Homo sapiens 36 A2M 2 Affinity Capture-MS Homo sapiens 37 NONO 4841 Affinity Capture-MS Homo sapiens 38 STAU1 6780 Affinity Capture-MS Homo sapiens 39 PON1 5444 Affinity Capture-MS Homo sapiens 40 ORM2   Affinity Capture-MS Homo sapiens 41 POC1A   Affinity Capture-MS Homo sapiens 42 COMP 1311 Affinity Capture-MS Homo sapiens 43 MATN2 4147 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 44 IGHG1 3500 Affinity Capture-MS Homo sapiens 45 APOA2 336 Affinity Capture-MS Homo sapiens 46 RPL36 25873 Affinity Capture-MS Homo sapiens 47 CCDC101   Affinity Capture-MS Homo sapiens 48 DHX9 1660 Affinity Capture-MS Homo sapiens 49 RRP1B 23076 Affinity Capture-MS Homo sapiens 50 FGG 2266 Affinity Capture-MS Homo sapiens 51 SERPINA3 12 Affinity Capture-MS Homo sapiens 52 IGHM 3507 Affinity Capture-MS Homo sapiens 53 ATXN1 6310 Two-hybrid Homo sapiens 54 CACNA1A   Two-hybrid Homo sapiens 55 CBFA2T3   Two-hybrid Homo sapiens 56 IGHA1 3493 Affinity Capture-MS Homo sapiens 57 MATN4 8785 Reconstituted Complex Homo sapiens 58 APOB 338 Affinity Capture-MS Homo sapiens 59 IGKC 3514 Affinity Capture-MS Homo sapiens 60 CP 1356 Affinity Capture-MS Homo sapiens 61 CUL3 8452 Affinity Capture-MS Homo sapiens 62 RPL3 6122 Affinity Capture-MS Homo sapiens 63 RPL7 6129 Affinity Capture-MS Homo sapiens 64 APCS 325 Affinity Capture-MS Homo sapiens 65 COL1A1 1277 Reconstituted Complex Homo sapiens 66 KLHL22 84861 Affinity Capture-MS Homo sapiens 67 MAP2K3 5606 Affinity Capture-MS Homo sapiens 68 NELL2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 69 RPS14 6208 Affinity Capture-MS Homo sapiens 70 NHP2L1 4809 Affinity Capture-MS Homo sapiens 71 PIK3CA 5290 Affinity Capture-MS Homo sapiens 72 CORO1A 11151 Affinity Capture-MS Homo sapiens 73 HOMER1   Affinity Capture-MS Homo sapiens 74 SERPING1 710 Affinity Capture-MS Homo sapiens 75 TRAF7   Affinity Capture-MS Homo sapiens 76 RPL6 6128 Affinity Capture-MS Homo sapiens 77 IGLL5 100423062 Affinity Capture-MS Homo sapiens 78 KLHL26   Affinity Capture-MS Homo sapiens 79 ATXN7   Two-hybrid Homo sapiens 80 NUFIP2 57532 Affinity Capture-MS Homo sapiens 81 HBA2 3040 Affinity Capture-MS Homo sapiens 82 C4A 720 Affinity Capture-MS Homo sapiens 83 THRAP3 9967 Affinity Capture-MS Homo sapiens 84 IGHG2 3501 Affinity Capture-MS Homo sapiens 85 HPR 3250 Affinity Capture-MS Homo sapiens 86 RPL34 6164 Affinity Capture-MS Homo sapiens 87 TF 7018 Affinity Capture-MS Homo sapiens 88 ATXN1L   Two-hybrid Homo sapiens 89 ITIH1 3697 Affinity Capture-MS Homo sapiens 90 RTCB 51493 Affinity Capture-MS Homo sapiens 91 TRIP4 9325 Affinity Capture-MS Homo sapiens 92 RPL23 9349 Affinity Capture-MS Homo sapiens 93 RPL8 6132 Affinity Capture-MS Homo sapiens 94 BANP   Affinity Capture-MS Homo sapiens 95 IGHG3 3502 Affinity Capture-MS Homo sapiens 96 CFB 629 Affinity Capture-MS Homo sapiens 97 CFH 3075 Affinity Capture-MS Homo sapiens 98 APOA1 335 Affinity Capture-MS Homo sapiens View the network

Pathways in which MATN2 is involved

No pathways found