Gene description for NPM1
Gene name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Gene symbol NPM1
Other names/aliases B23
NPM
Species Homo sapiens
 Database cross references - NPM1
ExoCarta ExoCarta_4869
Vesiclepedia VP_4869
Entrez Gene 4869
HGNC 7910
MIM 164040
UniProt P06748  
 NPM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 35918900    
Breast cancer cells 35918900    
Cholangiocarcinoma cells 31054213    
Cholangiocarcinoma cells 31054213    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 15326289    
 Gene ontology annotations for NPM1
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 IDA
    chromatin binding GO:0003682 IBA
    transcription coactivator activity GO:0003713 IDA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    protein kinase inhibitor activity GO:0004860 IDA
    protein binding GO:0005515 IPI
    rRNA binding GO:0019843 IPI
    protein kinase binding GO:0019901 IPI
    Tat protein binding GO:0030957 IDA
    histone binding GO:0042393 IBA
    histone binding GO:0042393 IDA
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 IPI
    ribosomal large subunit binding GO:0043023 IDA
    ribosomal small subunit binding GO:0043024 IDA
    NF-kappaB binding GO:0051059 IDA
    unfolded protein binding GO:0051082 IDA
    molecular adaptor activity GO:0060090 IDA
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    ribosomal large subunit export from nucleus GO:0000055 IBA
    ribosomal small subunit export from nucleus GO:0000056 IBA
    DNA repair GO:0006281 IDA
    nucleosome assembly GO:0006334 IDA
    chromatin remodeling GO:0006338 IBA
    protein import into nucleus GO:0006606 IDA
    intracellular protein transport GO:0006886 TAS
    nucleocytoplasmic transport GO:0006913 IDA
    nucleocytoplasmic transport GO:0006913 TAS
    centrosome cycle GO:0007098 IMP
    signal transduction GO:0007165 NAS
    protein localization GO:0008104 IDA
    positive regulation of cell population proliferation GO:0008284 IDA
    negative regulation of cell population proliferation GO:0008285 IMP
    regulation of centrosome duplication GO:0010824 IBA
    negative regulation of centrosome duplication GO:0010826 IMP
    macrophage differentiation GO:0030225 IDA
    regulation of endodeoxyribonuclease activity GO:0032071 IDA
    cellular response to UV GO:0034644 IDA
    ribosome assembly GO:0042255 TAS
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal small subunit biogenesis GO:0042274 IBA
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 NAS
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 IDA
    positive regulation of translation GO:0045727 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    regulation of centriole replication GO:0046599 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    regulation of endoribonuclease activity GO:0060699 IDA
    regulation of eIF2 alpha phosphorylation by dsRNA GO:0060735 IDA
    cellular senescence GO:0090398 IMP
    regulation of mRNA stability involved in cellular response to UV GO:1902629 IMP
    positive regulation of cell cycle G2/M phase transition GO:1902751 IDA
Subcellular Localization
    granular component GO:0001652 TAS
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 HDA
    membrane GO:0016020 HDA
    spindle pole centrosome GO:0031616 IDA
    protein-containing complex GO:0032991 IDA
    protein-DNA complex GO:0032993 IDA
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified NPM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1333
MISEV standards
Biophysical techniques
✔
CD81|EPCAM|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
Western blotting
6
Experiment ID 1335
MISEV standards
Biophysical techniques
✔
EPCAM|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
7
Experiment ID 1225
MISEV standards
✔
EM
Biophysical techniques
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 31054213    
Organism Homo sapiens
Experiment description Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma
Authors "Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C."
Journal name Proteomics
Publication year 2019