Gene description for EDC4
Gene name enhancer of mRNA decapping 4
Gene symbol EDC4
Other names/aliases GE1
Ge-1
HEDL5
HEDLS
RCD-8
RCD8
Species Homo sapiens
 Database cross references - EDC4
ExoCarta ExoCarta_23644
Entrez Gene 23644
HGNC 17157
MIM 606030
UniProt Q6P2E9  
 EDC4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for EDC4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    biological_process GO:0008150 ND
    exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay GO:0043928 TAS
    gene expression GO:0010467 TAS
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
Subcellular Localization
    cytoplasmic mRNA processing body GO:0000932 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
    membrane GO:0016020 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified EDC4 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EDC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which EDC4 is involved
PathwayEvidenceSource
mRNA decay by 5' to 3' exoribonuclease TAS Reactome





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