Gene description for DDX1
Gene name DEAD (Asp-Glu-Ala-Asp) box helicase 1
Gene symbol DDX1
Other names/aliases DBP-RB
UKVH5d
Species Homo sapiens
 Database cross references - DDX1
ExoCarta ExoCarta_1653
Entrez Gene 1653
HGNC 2734
MIM 601257
UniProt Q92499  
 DDX1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for DDX1
Molecular Function
    protein binding GO:0005515 IPI
    ATP-dependent RNA helicase activity GO:0004004 IBA
    DNA/RNA helicase activity GO:0033677 IDA
    poly(A) RNA binding GO:0044822 IDA
    nuclease activity GO:0004518 IDA
    ATP binding GO:0005524 IEA
    RNA helicase activity GO:0003724 TAS
    poly(A) binding GO:0008143 IDA
    DNA binding GO:0003677 IEA
    transcription cofactor activity GO:0003712 IDA
    exonuclease activity GO:0004527 IEA
    chromatin binding GO:0003682 IDA
    ATP-dependent helicase activity GO:0008026 IDA
    double-stranded RNA binding GO:0003725 IEA
Biological Process
    spliceosomal complex assembly GO:0000245 NAS
    double-strand break repair GO:0006302 IDA
    multicellular organismal development GO:0007275 IEP
    regulation of transcription, DNA-templated GO:0006355 IEA
    DNA duplex unwinding GO:0032508 IDA
    response to virus GO:0009615 IEA
    nucleic acid phosphodiester bond hydrolysis GO:0090305 IEA
    tRNA splicing, via endonucleolytic cleavage and ligation GO:0006388 IMP
    RNA secondary structure unwinding GO:0010501 IBA
    response to exogenous dsRNA GO:0043330 IEA
    transcription, DNA-templated GO:0006351 IEA
    regulation of translational initiation GO:0006446 NAS
Subcellular Localization
    cytoplasmic stress granule GO:0010494 IDA
    ribonucleoprotein complex GO:0030529 IDA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    tRNA-splicing ligase complex GO:0072669 IDA
    cleavage body GO:0071920 IDA
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified DDX1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DDX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPK 3190
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 VCL 7414
Affinity Capture-MS Homo sapiens
3 CSTF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DDX1 is involved
No pathways found





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