Gene description for NBN
Gene name nibrin
Gene symbol NBN
Other names/aliases AT-V1
AT-V2
ATV
NBS
NBS1
P95
Species Homo sapiens
 Database cross references - NBN
ExoCarta ExoCarta_4683
Vesiclepedia VP_4683
Entrez Gene 4683
HGNC 7652
MIM 602667
UniProt O60934  
 NBN identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NBN
Molecular Function
    damaged DNA binding GO:0003684 IBA
    damaged DNA binding GO:0003684 IC
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IPI
    protein serine/threonine kinase activator activity GO:0043539 IDA
    phosphorylation-dependent protein binding GO:0140031 IDA
    DNA-binding transcription factor binding GO:0140297 IPI
    chromatin-protein adaptor activity GO:0140463 IDA
Biological Process
    DNA damage checkpoint signaling GO:0000077 IDA
    telomere maintenance GO:0000723 IMP
    double-strand break repair via homologous recombination GO:0000724 IBA
    double-strand break repair via homologous recombination GO:0000724 IDA
    DNA double-strand break processing GO:0000729 IDA
    DNA double-strand break processing GO:0000729 NAS
    blastocyst growth GO:0001832 IEA
    double-strand break repair GO:0006302 IDA
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IBA
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IDA
    neuroblast proliferation GO:0007405 IEA
    regulation of DNA-templated DNA replication initiation GO:0030174 TAS
    DNA damage response, signal transduction by p53 class mediator GO:0030330 TAS
    protection from non-homologous end joining at telomere GO:0031848 IDA
    telomeric 3' overhang formation GO:0031860 IMP
    positive regulation of protein autophosphorylation GO:0031954 IDA
    positive regulation of telomere maintenance GO:0032206 IMP
    DNA duplex unwinding GO:0032508 IBA
    DNA duplex unwinding GO:0032508 IMP
    positive regulation of kinase activity GO:0033674 IDA
    homologous recombination GO:0035825 NAS
    telomere maintenance in response to DNA damage GO:0043247 IDA
    mitotic G2/M transition checkpoint GO:0044818 NAS
    isotype switching GO:0045190 IEA
    neuromuscular process controlling balance GO:0050885 IEA
    meiotic cell cycle GO:0051321 IEA
    regulation of cell cycle GO:0051726 TAS
    R-loop processing GO:0062176 IDA
    protein K63-linked ubiquitination GO:0070534 ISS
    t-circle formation GO:0090656 IMP
    telomere maintenance via telomere trimming GO:0090737 IGI
    intrinsic apoptotic signaling pathway GO:0097193 IEA
    double-strand break repair via alternative nonhomologous end joining GO:0097681 IDA
    DNA strand resection involved in replication fork processing GO:0110025 IDA
    DNA strand resection involved in replication fork processing GO:0110025 NAS
    negative regulation of telomere capping GO:1904354 IMP
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISS
    protein localization to site of double-strand break GO:1990166 IDA
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    chromosome, telomeric region GO:0000781 IDA
    chromosome, telomeric region GO:0000781 IDA
    chromosome, telomeric region GO:0000781 ISS
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    replication fork GO:0005657 IEA
    nucleolus GO:0005730 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    PML body GO:0016605 IDA
    Mre11 complex GO:0030870 IBA
    Mre11 complex GO:0030870 IDA
    Mre11 complex GO:0030870 IPI
    Mre11 complex GO:0030870 TAS
    site of double-strand break GO:0035861 IDA
    site of double-strand break GO:0035861 IDA
    nuclear inclusion body GO:0042405 IDA
    BRCA1-C complex GO:0070533 IPI
    chromosomal region GO:0098687 NAS
 Experiment description of studies that identified NBN in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NBN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 LIG4 3981
Co-fractionation Homo sapiens
4 TP53BP1 7158
Co-localization Homo sapiens
Co-fractionation Homo sapiens
5 HOOK1  
Proximity Label-MS Homo sapiens
6 UBE2A  
Affinity Capture-Western Homo sapiens
7 GSTP1 2950
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PAXIP1  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
9 MSH6 2956
Co-purification Homo sapiens
Co-fractionation Homo sapiens
10 RPA2 6118
Affinity Capture-MS Homo sapiens
11 BRCA1 672
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 C9orf78 51759
Affinity Capture-MS Homo sapiens
13 NABP2  
Affinity Capture-Western Homo sapiens
14 NUMA1 4926
Affinity Capture-MS Homo sapiens
15 EYA2  
Proximity Label-MS Homo sapiens
16 TERF2IP 54386
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
17 PRKDC 5591
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
18 DPPA4  
Affinity Capture-MS Homo sapiens
19 PELI1 57162
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 UBE2O 63893
Affinity Capture-Western Homo sapiens
21 H2afx 15270
Co-localization Mus musculus
22 RNF8  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 ESRRG  
Affinity Capture-MS Homo sapiens
24 MDC1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Co-fractionation Homo sapiens
25 POU5F1  
Affinity Capture-MS Homo sapiens
26 BRPF3  
Affinity Capture-MS Homo sapiens
27 TAX1BP1 8887
Co-fractionation Homo sapiens
28 TLK1  
Biochemical Activity Homo sapiens
29 RGS20 8601
Affinity Capture-MS Homo sapiens
30 ATRX 546
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
31 DYNLT1 6993
Proximity Label-MS Homo sapiens
32 WRN 7486
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 XRCC4  
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
34 BRIP1  
Affinity Capture-Western Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
36 MRE11A 4361
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 ERCC6  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
39 BLM 641
Co-purification Homo sapiens
Co-fractionation Homo sapiens
40 ATF2  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
41 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
42 VRK1 7443
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
43 RAB43 339122
Affinity Capture-MS Homo sapiens
44 MDM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 PBXIP1 57326
Affinity Capture-MS Homo sapiens
46 BARD1 580
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
47 SLX4  
Affinity Capture-Western Homo sapiens
48 AIM2  
Affinity Capture-MS Homo sapiens
49 RAD18  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 CHEK1  
Phenotypic Enhancement Homo sapiens
51 SUMO2 6613
Reconstituted Complex Homo sapiens
52 C15orf26  
Affinity Capture-MS Homo sapiens
53 CELA2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RFC4 5984
Co-fractionation Homo sapiens
55 DDX1 1653
Affinity Capture-Western Homo sapiens
56 DNAJC5G  
Proximity Label-MS Homo sapiens
57 NELFE 7936
Affinity Capture-MS Homo sapiens
58 DNAJC30  
Proximity Label-MS Homo sapiens
59 H2AFX 3014
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
60 CD3EAP  
Affinity Capture-Western Homo sapiens
61 RECQL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
62 UBE2N 7334
Reconstituted Complex Homo sapiens
63 IPO5 3843
Affinity Capture-MS Homo sapiens
64 HIST1H3A 8350
Affinity Capture-Western Homo sapiens
65 FANCD2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
66 MSH2 4436
Co-purification Homo sapiens
Co-fractionation Homo sapiens
67 NR3C1 2908
Proximity Label-MS Homo sapiens
68 FGF12  
Affinity Capture-MS Homo sapiens
69 CD6 923
Affinity Capture-MS Homo sapiens
70 SKP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 RICTOR 253260
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 CCNE1  
Affinity Capture-Western Homo sapiens
73 ATM 472
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
74 ATRIP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
75 MDM4  
Affinity Capture-Western Homo sapiens
76 HIST3H3 8290
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
77 RFC1 5981
Co-purification Homo sapiens
Co-fractionation Homo sapiens
78 DCLRE1C  
Affinity Capture-Western Homo sapiens
79 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
81 MTOR 2475
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
82 MAPKAP1 79109
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
83 KAT5  
Affinity Capture-Western Homo sapiens
84 HSPA4 3308
Affinity Capture-MS Homo sapiens
85 BICD1 636
Proximity Label-MS Homo sapiens
86 BMI1  
Affinity Capture-Western Homo sapiens
87 BMP1 649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 NANOG  
Affinity Capture-MS Homo sapiens
89 HOOK3 84376
Proximity Label-MS Homo sapiens
90 PYHIN1  
Affinity Capture-MS Homo sapiens
91 XRCC5 7520
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
92 RBBP8  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
93 UBE2D1 7321
Reconstituted Complex Homo sapiens
94 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
95 TERF2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
96 PRKY  
Affinity Capture-MS Homo sapiens
97 LIG3 3980
Affinity Capture-MS Homo sapiens
98 TOPBP1 11073
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
99 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 TUBG1 7283
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
101 PHRF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
102 RPA1 6117
Affinity Capture-MS Homo sapiens
103 MEN1 4221
Affinity Capture-MS Homo sapiens
104 HNF4A  
Proximity Label-MS Homo sapiens
105 MLH1 4292
Co-purification Homo sapiens
Co-fractionation Homo sapiens
106 SIRT1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
107 RAD50 10111
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
108 FAM219A 203259
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 MCPH1  
Co-localization Homo sapiens
110 TERF1 7013
Two-hybrid Homo sapiens
111 NCL 4691
Reconstituted Complex Homo sapiens
112 UBE2B 7320
Affinity Capture-Western Homo sapiens
113 XRCC6 2547
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
114 SNW1 22938
Affinity Capture-MS Homo sapiens
115 KRAS 3845
Synthetic Lethality Homo sapiens
116 CCT8L2  
Affinity Capture-MS Homo sapiens
117 RASA2 5922
Affinity Capture-MS Homo sapiens
118 EP300 2033
Affinity Capture-MS Homo sapiens
119 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NBN is involved
PathwayEvidenceSource
Cell Cycle IEA Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function TAS Reactome
Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function TAS Reactome
Defective homologous recombination repair (HRR) due to BRCA1 loss of function TAS Reactome
Defective homologous recombination repair (HRR) due to BRCA2 loss of function TAS Reactome
Defective homologous recombination repair (HRR) due to PALB2 loss of function TAS Reactome
Disease TAS Reactome
Diseases of DNA Double-Strand Break Repair TAS Reactome
Diseases of DNA repair TAS Reactome
DNA Damage/Telomere Stress Induced Senescence TAS Reactome
DNA Double Strand Break Response TAS Reactome
DNA Double-Strand Break Repair TAS Reactome
DNA Repair TAS Reactome
G2/M Checkpoints TAS Reactome
G2/M DNA damage checkpoint TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
HDR through Homologous Recombination (HRR) TAS Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) TAS Reactome
HDR through MMEJ (alt-NHEJ) TAS Reactome
HDR through Single Strand Annealing (SSA) TAS Reactome
Homologous DNA Pairing and Strand Exchange TAS Reactome
Homology Directed Repair TAS Reactome
Impaired BRCA2 binding to PALB2 TAS Reactome
Impaired BRCA2 binding to RAD51 TAS Reactome
Meiosis IEA Reactome
Meiotic recombination IEA Reactome
Nonhomologous End-Joining (NHEJ) TAS Reactome
Presynaptic phase of homologous DNA pairing and strand exchange TAS Reactome
Processing of DNA double-strand break ends TAS Reactome
Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Activity through Phosphorylation TAS Reactome
Reproduction IEA Reactome
Resolution of D-Loop Structures TAS Reactome
Resolution of D-loop Structures through Holliday Junction Intermediates TAS Reactome
Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Sensing of DNA Double Strand Breaks TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome





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