Gene description for NBN
Gene name nibrin
Gene symbol NBN
Other names/aliases AT-V1
AT-V2
ATV
NBS
NBS1
P95
Species Homo sapiens
 Database cross references - NBN
ExoCarta ExoCarta_4683
Vesiclepedia VP_4683
Entrez Gene 4683
HGNC 7652
MIM 602667
UniProt O60934  
 NBN identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NBN
Molecular Function
    transcription factor binding GO:0008134 IPI
    protein N-terminus binding GO:0047485 IPI
    protein binding GO:0005515 IPI
    damaged DNA binding GO:0003684 IC
    ATP-dependent DNA helicase activity GO:0004003 IMP
Biological Process
    positive regulation of protein autophosphorylation GO:0031954 IDA
    mitotic G2 DNA damage checkpoint GO:0007095 IDA
    blastocyst growth GO:0001832 IEA
    isotype switching GO:0045190 IEA
    DNA repair GO:0006281 TAS
    intrinsic apoptotic signaling pathway GO:0097193 IEA
    double-strand break repair GO:0006302 TAS
    meiotic cell cycle GO:0051321 IEA
    DNA duplex unwinding GO:0032508 IMP
    double-strand break repair via homologous recombination GO:0000724 TAS
    telomere maintenance GO:0000723 IMP
    positive regulation of kinase activity GO:0033674 IDA
    cell proliferation GO:0008283 IEA
    DNA damage checkpoint GO:0000077 IDA
    mitotic cell cycle checkpoint GO:0007093 IDA
    neuromuscular process controlling balance GO:0050885 IEA
    DNA damage response, signal transduction by p53 class mediator GO:0030330 TAS
    regulation of DNA-dependent DNA replication initiation GO:0030174 TAS
    cell cycle arrest GO:0007050 TAS
Subcellular Localization
    nuclear inclusion body GO:0042405 IDA
    site of double-strand break GO:0035861 IDA
    replication fork GO:0005657 IEA
    Mre11 complex GO:0030870 IDA
    nucleolus GO:0005730 IDA
    nuclear chromosome, telomeric region GO:0000784 IDA
    PML body GO:0016605 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified NBN in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NBN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLM 641
Co-purification Homo sapiens
2 ATR  
Protein-peptide Homo sapiens
3 RFC1 5981
Co-purification Homo sapiens
4 BRCA1 672
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-localization Homo sapiens
5 TERF1 7013
Two-hybrid Homo sapiens
6 RAD50 10111
Co-purification Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
7 ATM 472
Protein-peptide Homo sapiens
Co-purification Homo sapiens
8 MRE11A 4361
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
9 FANCD2  
Affinity Capture-Western Homo sapiens
10 MLH1 4292
Co-purification Homo sapiens
11 MDC1  
Affinity Capture-Western Homo sapiens
12 MSH6 2956
Co-purification Homo sapiens
13 MSH2 4436
Co-purification Homo sapiens
14 H2afx 15270
Co-localization Mus musculus
15 H2AFX 3014
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here