Gene description for TAX1BP1
Gene name Tax1 (human T-cell leukemia virus type I) binding protein 1
Gene symbol TAX1BP1
Other names/aliases CALCOCO3
T6BP
TXBP151
Species Homo sapiens
 Database cross references - TAX1BP1
ExoCarta ExoCarta_8887
Vesiclepedia VP_8887
Entrez Gene 8887
HGNC 11575
MIM 605326
UniProt Q86VP1  
 TAX1BP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Melanoma cells 22635005    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TAX1BP1
Molecular Function
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 ISS
    protein-macromolecule adaptor activity GO:0030674 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    autophagy GO:0006914 IEA
    apoptotic process GO:0006915 IEA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISS
    negative regulation of toll-like receptor 4 signaling pathway GO:0034144 IDA
    negative regulation of cytoplasmic pattern recognition receptor signaling pathway GO:0039532 IDA
    negative regulation of apoptotic process GO:0043066 IBA
    innate immune response GO:0045087 IEA
    protein localization to phagocytic vesicle GO:1905161 IDA
Subcellular Localization
    phagophore assembly site GO:0000407 IEA
    mitochondrion GO:0005739 IDA
    autophagosome GO:0005776 IEA
    cytosol GO:0005829 TAS
    cytoplasmic vesicle GO:0031410 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TAX1BP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 200
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|TSG101|Alix|GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22635005    
Organism Homo sapiens
Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Authors "Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, Garcia-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D."
Journal name Nat Med
Publication year 2012
Sample Melanoma cells
Sample name B16-F10
SK-MEL-202
SK-MEL035
SK-MEL-265
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TAX1BP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 FAM58A  
Affinity Capture-MS Homo sapiens
3 TNFAIP3 7128
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 MAP1LC3C  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 FHL5 9457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TNIP1 10318
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 DNAJC11 55735
Affinity Capture-MS Homo sapiens
8 UBE2A  
Affinity Capture-MS Homo sapiens
9 NRSN1  
Affinity Capture-MS Homo sapiens
10 SCNM1  
Two-hybrid Homo sapiens
11 BTF3 689
Affinity Capture-MS Homo sapiens
12 TSG101 7251
Two-hybrid Homo sapiens
13 SPSB4  
Affinity Capture-MS Homo sapiens
14 HSD17B3  
Affinity Capture-MS Homo sapiens
15 FAM189B  
Affinity Capture-MS Homo sapiens
16 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
17 LRRC73  
Affinity Capture-MS Homo sapiens
18 RIPK1 8737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 KDELR1 10945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RB1CC1 9821
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
21 APEX1 328
Affinity Capture-RNA Homo sapiens
22 MAP1LC3B 81631
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
23 EMILIN1 11117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 C18orf25 147339
Two-hybrid Homo sapiens
25 KIF5B 3799
Two-hybrid Homo sapiens
26 SLC5A12 159963
Affinity Capture-MS Homo sapiens
27 ORC5  
Two-hybrid Homo sapiens
28 CALCOCO2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
29 Cenpe  
Affinity Capture-MS Mus musculus
30 OTUD7B 56957
Affinity Capture-Western Homo sapiens
31 IKBKE 9641
Affinity Capture-Western Homo sapiens
32 TBC1D15 64786
Two-hybrid Homo sapiens
33 IGFN1  
Two-hybrid Homo sapiens
34 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MAVS 57506
Affinity Capture-Western Homo sapiens
36 RNF126  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TMEM136  
Affinity Capture-MS Homo sapiens
38 IQCF2  
Affinity Capture-MS Homo sapiens
39 GRIN3B  
Affinity Capture-MS Homo sapiens
40 RNF11 26994
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
41 MED4 29079
Affinity Capture-MS Homo sapiens
42 TBC1D17 79735
Two-hybrid Homo sapiens
43 RABGEF1 27342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CXXC1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 TOLLIP 54472
Proximity Label-MS Homo sapiens
46 RNF6  
Affinity Capture-MS Homo sapiens
47 MIB1 57534
Proximity Label-MS Homo sapiens
48 REEP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TCEA2  
Two-hybrid Homo sapiens
50 OPTN 10133
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 NBN 4683
Co-fractionation Homo sapiens
52 RNF115  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SSX2  
Two-hybrid Homo sapiens
54 Spred2  
Affinity Capture-MS Mus musculus
55 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
56 PLEKHB2 55041
Two-hybrid Homo sapiens
57 TNIP3  
Two-hybrid Homo sapiens
58 GABARAP 11337
Reconstituted Complex Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 RAD18  
Two-hybrid Homo sapiens
61 FOXC2  
Two-hybrid Homo sapiens
62 SLC22A23  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 WDYHV1  
Two-hybrid Homo sapiens
64 DYNLL2 140735
Affinity Capture-MS Homo sapiens
65 MRPL11 65003
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 RAD54L2  
Two-hybrid Homo sapiens
67 ITCH 83737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 GET4 51608
Affinity Capture-MS Homo sapiens
69 HSPB8 26353
Co-localization Homo sapiens
70 DAXX  
Two-hybrid Homo sapiens
71 VCP 7415
Affinity Capture-MS Homo sapiens
72 HNRNPD 3184
Affinity Capture-MS Homo sapiens
73 CUL3 8452
Affinity Capture-MS Homo sapiens
74 CTNNB1 1499
Affinity Capture-MS Homo sapiens
75 TRAF6 7189
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
76 TCL1A  
Two-hybrid Homo sapiens
77 FAM168A  
Two-hybrid Homo sapiens
78 FAM90A1  
Two-hybrid Homo sapiens
79 TRAF3 7187
Affinity Capture-Western Homo sapiens
80 FAM84B 157638
Two-hybrid Homo sapiens
81 CCDC85A  
Affinity Capture-MS Homo sapiens
82 MYO6 4646
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
83 UBASH3A 53347
Two-hybrid Homo sapiens
84 DLST 1743
Affinity Capture-MS Homo sapiens
85 PPAPDC1A  
Affinity Capture-MS Homo sapiens
86 KAT5  
Two-hybrid Homo sapiens
87 DSTN 11034
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
88 LGALS8 3964
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
89 RNF26  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
90 AR 367
Affinity Capture-MS Homo sapiens
91 RAB18 22931
Two-hybrid Homo sapiens
92 STARD13 90627
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
93 TSHZ3  
Two-hybrid Homo sapiens
94 AGO1  
Affinity Capture-RNA Homo sapiens
95 RFWD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 POLR1C 9533
Two-hybrid Homo sapiens
97 MRPL53  
Two-hybrid Homo sapiens
98 TRIM45  
Two-hybrid Homo sapiens
99 IKBKG 8517
Two-hybrid Homo sapiens
100 GABARAPL2 11345
Two-hybrid Homo sapiens
101 LITAF 9516
Two-hybrid Homo sapiens
102 UBA52 7311
Two-hybrid Homo sapiens
103 SQSTM1 8878
Proximity Label-MS Homo sapiens
104 TSEN2  
Two-hybrid Homo sapiens
105 CWF19L2  
Two-hybrid Homo sapiens
106 OR2G6  
Affinity Capture-MS Homo sapiens
107 OR5M3  
Affinity Capture-MS Homo sapiens
108 ALG6  
Affinity Capture-MS Homo sapiens
109 TBK1 29110
Affinity Capture-Western Homo sapiens
110 UBB 7314
Two-hybrid Homo sapiens
111 NMRK1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
112 GABRR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
113 UBTFL1  
Two-hybrid Homo sapiens
114 FAM161A  
Two-hybrid Homo sapiens
115 CBX8 57332
Two-hybrid Homo sapiens
116 TCAF1 9747
Two-hybrid Homo sapiens
117 KANK2 25959
Two-hybrid Homo sapiens
118 KXD1 79036
Affinity Capture-MS Homo sapiens
119 FAM189A2  
Two-hybrid Homo sapiens
120 TMEM31  
Affinity Capture-MS Homo sapiens
121 RAD23B 5887
Two-hybrid Homo sapiens
122 COG7 91949
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
123 LMO3  
Two-hybrid Homo sapiens
124 PLEKHN1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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