Gene description for RIPK1
Gene name receptor (TNFRSF)-interacting serine-threonine kinase 1
Gene symbol RIPK1
Other names/aliases RIP
RIP1
Species Homo sapiens
 Database cross references - RIPK1
ExoCarta ExoCarta_8737
Vesiclepedia VP_8737
Entrez Gene 8737
HGNC 10019
MIM 603453
UniProt Q13546  
 RIPK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RIPK1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    protein serine/threonine kinase activity GO:0004674 IDA
    ATP binding GO:0005524 IEA
    death domain binding GO:0070513 IPI
    identical protein binding GO:0042802 IPI
    protein kinase activity GO:0004672 IMP
    death receptor binding GO:0005123 IPI
    protein complex binding GO:0032403 IDA
Biological Process
    ripoptosome assembly involved in necroptotic process GO:1901026 IEA
    protein autophosphorylation GO:0046777 IDA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IEA
    protein heterooligomerization GO:0051291 IMP
    positive regulation of type I interferon production GO:0032481 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    response to tumor necrosis factor GO:0034612 IMP
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IMP
    programmed cell death GO:0012501 TAS
    programmed necrotic cell death GO:0097300 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IMP
    positive regulation of programmed cell death GO:0043068 IMP
    cellular response to tumor necrosis factor GO:0071356 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    ripoptosome assembly GO:0097343 IMP
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    regulation of ATP:ADP antiporter activity GO:0070926 IMP
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    necroptotic process GO:0070266 TAS
    positive regulation of necroptotic process GO:0060545 IMP
    apoptotic process GO:0006915 TAS
    peptidyl-serine autophosphorylation GO:0036289 IDA
    protein homooligomerization GO:0051260 IDA
    positive regulation of reactive oxygen species metabolic process GO:2000379 TAS
    amyloid fibril formation GO:1990000 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    toll-like receptor signaling pathway GO:0002224 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    cellular response to growth factor stimulus GO:0071363 IEA
    positive regulation of macrophage differentiation GO:0045651 IMP
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of interleukin-8 production GO:0032757 IDA
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    innate immune response GO:0045087 TAS
    necroptotic signaling pathway GO:0097527 ISS
    positive regulation of JNK cascade GO:0046330 IDA
    activation of JUN kinase activity GO:0007257 TAS
    cellular protein catabolic process GO:0044257 IDA
    death-inducing signaling complex assembly GO:0071550 TAS
    T cell apoptotic process GO:0070231 ISS
    regulation of necrotic cell death GO:0010939 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
Subcellular Localization
    ripoptosome GO:0097342 IDA
    receptor complex GO:0043235 IDA
    death-inducing signaling complex GO:0031264 IDA
    cytosol GO:0005829 TAS
    membrane raft GO:0045121 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified RIPK1 in exosomes
1
Experiment ID 489
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
EV Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
Canx
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
EV Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RIPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAF2 7186
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CRADD  
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
3 IKBKG 8517
Affinity Capture-Western Homo sapiens
4 RIPK3 11035
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 TNFRSF1A 7132
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 MAP3K14  
Invivo Homo sapiens
7 TRIAD3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
8 CASP8 841
Invivo Homo sapiens
9 TRADD 8717
Invivo Homo sapiens
Invitro Homo sapiens
10 SQSTM1 8878
Invivo Homo sapiens
Invitro Homo sapiens
11 MAP3K3 4215
Invivo Homo sapiens
12 CFLAR  
Affinity Capture-Western Homo sapiens
13 EGFR 1956
Invivo Homo sapiens
14 TRAF3 7187
Invivo Homo sapiens
Two-hybrid Homo sapiens
15 TRAF5  
Affinity Capture-Western Homo sapiens
16 TRPC4AP  
Affinity Capture-Western Homo sapiens
17 FAS 355
Two-hybrid Homo sapiens
18 FADD 8772
Two-hybrid Homo sapiens
19 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
20 TRAF1 7185
Invivo Homo sapiens
Invitro Homo sapiens
21 TRIF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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