Gene description for MLKL
Gene name mixed lineage kinase domain-like
Gene symbol MLKL
Other names/aliases hMLKL
Species Homo sapiens
 Database cross references - MLKL
ExoCarta ExoCarta_197259
Vesiclepedia VP_197259
Entrez Gene 197259
HGNC 26617
MIM 615153
UniProt Q8NB16  
 MLKL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 36063142    
Colorectal cancer cells 36063142    
Colorectal cancer cells 36063142    
Embryonic kidney cells 36063142    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MLKL
Molecular Function
    protein kinase activity GO:0004672 ISS
    protein serine/threonine kinase activity GO:0004674 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein kinase binding GO:0019901 IEA
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IDA
Biological Process
    cell surface receptor signaling pathway GO:0007166 IEA
    defense response to virus GO:0051607 ISS
    protein homotrimerization GO:0070207 IDA
    necroptotic process GO:0070266 IMP
    necroptotic signaling pathway GO:0097527 IBA
    necroptotic signaling pathway GO:0097527 IMP
    execution phase of necroptosis GO:0097528 IDA
    execution phase of necroptosis GO:0097528 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    cell junction GO:0030054 IDA
 Experiment description of studies that identified MLKL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1343
MISEV standards
Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36063142    
Organism Homo sapiens
Experiment description Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL
Authors "Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B."
Journal name J Extracell Vesicles
Publication year 2022
Sample Colorectal cancer cells
Sample name HT-29
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 1344
MISEV standards
Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36063142    
Organism Homo sapiens
Experiment description Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL
Authors "Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B."
Journal name J Extracell Vesicles
Publication year 2022
Sample Colorectal cancer cells
Sample name HT-29
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 1346
MISEV standards
Biophysical techniques
✘ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36063142    
Organism Homo sapiens
Experiment description Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL
Authors "Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B."
Journal name J Extracell Vesicles
Publication year 2022
Sample Colorectal cancer cells
Sample name HT-29
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 1347
MISEV standards
Biophysical techniques
✘ Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36063142    
Organism Homo sapiens
Experiment description Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL
Authors "Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B."
Journal name J Extracell Vesicles
Publication year 2022
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MLKL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USP2  
Biochemical Activity Homo sapiens
2 TRAF2 7186
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 VPS28 51160
Affinity Capture-Western Homo sapiens
4 MVB12B 89853
Affinity Capture-Western Homo sapiens
5 THOC3  
Two-hybrid Homo sapiens
6 NDUFB7 4713
Two-hybrid Homo sapiens
7 PITPNA 5306
Affinity Capture-MS Homo sapiens
8 TRIM66  
Affinity Capture-MS Homo sapiens
9 CASP8 841
Affinity Capture-MS Homo sapiens
10 TSG101 7251
Affinity Capture-Western Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 TRIM33 51592
Affinity Capture-MS Homo sapiens
13 BRD2  
Affinity Capture-MS Homo sapiens
14 S100A10 6281
Two-hybrid Homo sapiens
15 ZBP1  
Affinity Capture-Western Homo sapiens
16 SRA1 10011
Affinity Capture-MS Homo sapiens
17 EXO1  
Negative Genetic Homo sapiens
18 ZNF281  
Affinity Capture-MS Homo sapiens
19 CDK1 983
Two-hybrid Homo sapiens
20 TRIM24  
Affinity Capture-MS Homo sapiens
21 RIPK3 11035
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 SP110  
Affinity Capture-MS Homo sapiens
23 GLG1 2734
Affinity Capture-MS Homo sapiens
24 ZMYND8 23613
Affinity Capture-MS Homo sapiens
25 CECR2  
Affinity Capture-MS Homo sapiens
26 RAD18  
Two-hybrid Homo sapiens
27 BRD1 23774
Affinity Capture-MS Homo sapiens
28 RIPK1 8737
Affinity Capture-Western Homo sapiens
29 SNW1 22938
Affinity Capture-MS Homo sapiens
30 STAMBP 10617
Biochemical Activity Homo sapiens
31 ITCH 83737
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 ANAPC11  
Two-hybrid Homo sapiens
33 LRCH3 84859
Affinity Capture-MS Homo sapiens
34 CEBPA  
Protein-peptide Homo sapiens
35 PARP16 54956
Affinity Capture-MS Homo sapiens
36 CYLD  
Biochemical Activity Homo sapiens
37 BRD4 23476
Affinity Capture-MS Homo sapiens
38 SP100 6672
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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