Gene description for RIPK3
Gene name receptor-interacting serine-threonine kinase 3
Gene symbol RIPK3
Other names/aliases RIP3
Species Homo sapiens
 Database cross references - RIPK3
ExoCarta ExoCarta_11035
Vesiclepedia VP_11035
Entrez Gene 11035
HGNC 10021
MIM 605817
UniProt Q9Y572  
 RIPK3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RIPK3
Molecular Function
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 IMP
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 ISS
    transcription coactivator activity GO:0003713 TAS
    identical protein binding GO:0042802 IPI
    NF-kappaB-inducing kinase activity GO:0004704 IEA
    protein kinase activity GO:0004672 IMP
Biological Process
    innate immune response GO:0045087 TAS
    regulation of interferon-gamma production GO:0032649 ISS
    regulation of activation-induced cell death of T cells GO:0070235 ISS
    toll-like receptor signaling pathway GO:0002224 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    NIK/NF-kappaB signaling GO:0038061 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    regulation of adaptive immune response GO:0002819 ISS
    programmed cell death GO:0012501 TAS
    regulation of T cell mediated cytotoxicity GO:0001914 ISS
    positive regulation of oxidoreductase activity GO:0051353 IEA
    cellular protein modification process GO:0006464 TAS
    protein autophosphorylation GO:0046777 IDA
    T cell differentiation in thymus GO:0033077 ISS
    thymus development GO:0048538 ISS
    amyloid fibril formation GO:1990000 IMP
    necroptotic process GO:0070266 TAS
    positive regulation of protein deacetylation GO:0090312 IEA
    I-kappaB kinase/NF-kappaB signaling GO:0007249 IEA
    regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation GO:2000452 ISS
    activation of protein kinase activity GO:0032147 IMP
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    positive regulation of ligase activity GO:0051351 IEA
    positive regulation of necroptotic process GO:0060545 IMP
    T cell homeostasis GO:0043029 ISS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
    spleen development GO:0048536 ISS
    regulation of activated T cell proliferation GO:0046006 ISS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    positive regulation of reactive oxygen species metabolic process GO:2000379 IEA
    protein homooligomerization GO:0051260 IDA
    programmed necrotic cell death GO:0097300 TAS
    protein heterooligomerization GO:0051291 IMP
    signal transduction GO:0007165 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    lymph node development GO:0048535 ISS
    positive regulation of phosphatase activity GO:0010922 IMP
    apoptotic signaling pathway GO:0097190 TAS
    positive regulation of type I interferon production GO:0032481 TAS
Subcellular Localization
    plasma membrane GO:0005886 IEA
    cytosol GO:0005829 TAS
    ripoptosome GO:0097342 IEA
    mitochondrion GO:0005739 IEA
 Experiment description of studies that identified RIPK3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RIPK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAF2 7186
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 RIPK1 8737
Invivo Homo sapiens
Two-hybrid Homo sapiens
3 TNFRSF1A 7132
Invivo Homo sapiens
4 TRIF  
Affinity Capture-Western Homo sapiens
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