Gene description for FADD
Gene name Fas (TNFRSF6)-associated via death domain
Gene symbol FADD
Other names/aliases GIG3
MORT1
Species Homo sapiens
 Database cross references - FADD
ExoCarta ExoCarta_8772
Vesiclepedia VP_8772
Entrez Gene 8772
HGNC 3573
MIM 602457
UniProt Q13158  
 FADD identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for FADD
Molecular Function
    protein binding GO:0005515 IPI
    tumor necrosis factor receptor binding GO:0005164 IEA
    identical protein binding GO:0042802 IPI
    tumor necrosis factor receptor superfamily binding GO:0032813 IPI
    protein complex binding GO:0032403 IEA
    protease binding GO:0002020 IPI
    death effector domain binding GO:0035877 IPI
    death receptor binding GO:0005123 IEA
Biological Process
    cell surface receptor signaling pathway GO:0007166 TAS
    positive regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902043 IEA
    negative regulation of necroptotic process GO:0060546 IEA
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 TAS
    positive regulation of proteolysis GO:0045862 IDA
    T cell homeostasis GO:0043029 ISS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    positive regulation of tumor necrosis factor production GO:0032760 IDA
    cellular response to mechanical stimulus GO:0071260 IEP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    apoptotic process GO:0006915 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    programmed necrotic cell death GO:0097300 TAS
    activation of cysteine-type endopeptidase activity GO:0097202 IDA
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    protein heterooligomerization GO:0051291 IEA
    necroptotic signaling pathway GO:0097527 IMP
    thymus development GO:0048538 ISS
    lymph node development GO:0048535 ISS
    apoptotic signaling pathway GO:0097190 TAS
    viral process GO:0016032 IEA
    spleen development GO:0048536 ISS
    defense response to virus GO:0051607 IMP
    positive regulation of T cell mediated cytotoxicity GO:0001916 ISS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    positive regulation of type I interferon-mediated signaling pathway GO:0060340 IMP
    death-inducing signaling complex assembly GO:0071550 TAS
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IEA
    necroptotic process GO:0070266 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 TAS
    programmed cell death GO:0012501 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097296 TAS
    tumor necrosis factor-mediated signaling pathway GO:0033209 TAS
    positive regulation of interleukin-8 production GO:0032757 IDA
    toll-like receptor signaling pathway GO:0002224 TAS
    positive regulation of adaptive immune response GO:0002821 ISS
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    TRAIL-activated apoptotic signaling pathway GO:0036462 IDA
    positive regulation of activated T cell proliferation GO:0042104 ISS
    positive regulation of macrophage differentiation GO:0045651 IMP
    innate immune response GO:0045087 TAS
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IMP
    regulation of necrotic cell death GO:0010939 TAS
    negative regulation of activation-induced cell death of T cells GO:0070236 ISS
    T cell differentiation in thymus GO:0033077 ISS
    positive regulation of interferon-gamma production GO:0032729 ISS
    positive regulation of apoptotic process GO:0043065 IMP
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    motor neuron apoptotic process GO:0097049 IEA
    positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation GO:2000454 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    membrane raft GO:0045121 IEA
    neuron projection GO:0043005 IEA
    death-inducing signaling complex GO:0031264 TAS
    ripoptosome GO:0097342 IDA
    cell body GO:0044297 IEA
    CD95 death-inducing signaling complex GO:0031265 IDA
 Experiment description of studies that identified FADD in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for FADD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FASLG 356
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
2 CASP8 841
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 CFLAR  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 DAP3 7818
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 FAS 355
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 LRDD  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 NACA 4666
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 EZR 7430
Co-purification Homo sapiens
9 HIPK3 10114
Affinity Capture-Western Homo sapiens
10 ABCA1 19
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 PRKCZ 5590
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
12 TRADD 8717
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 BID  
Co-purification Homo sapiens
14 IRAK1  
Invitro Homo sapiens
15 MOB4 25843
Two-hybrid Homo sapiens
16 RIPK1 8737
Two-hybrid Homo sapiens
17 ARHGDIA 396
Co-purification Homo sapiens
18 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
19 XPO5 57510
Affinity Capture-MS Homo sapiens
20 DAPK1 1612
Affinity Capture-Western Homo sapiens
21 MYD88 4615
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
22 CASP10  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
23 MBD4  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
24 CASP8AP2  
Invivo Homo sapiens
25 MSN 4478
Co-purification Homo sapiens
26 RHOA 387
Co-purification Homo sapiens
27 MAPK8 5599
Co-purification Homo sapiens
28 PEA15 8682
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
29 DEDD  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
30 TNFRSF1A 7132
Co-purification Homo sapiens
31 RYBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
32 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
33 SUMO1 7341
Two-hybrid Homo sapiens
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