Gene description for NOL3
Gene name nucleolar protein 3 (apoptosis repressor with CARD domain)
Gene symbol NOL3
Other names/aliases ARC
FCM
MYP
NOP
NOP30
Species Homo sapiens
 Database cross references - NOL3
ExoCarta ExoCarta_8996
Vesiclepedia VP_8996
Entrez Gene 8996
HGNC 7869
MIM 605235
UniProt O60936  
 NOL3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NOL3
Molecular Function
    RNA binding GO:0003723 TAS
    death receptor binding GO:0005123 IBA
    calcium ion binding GO:0005509 IMP
    protein binding GO:0005515 IPI
    death effector domain binding GO:0035877 IEA
    identical protein binding GO:0042802 IPI
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IBA
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IDA
    caspase binding GO:0089720 IBA
    caspase binding GO:0089720 IPI
Biological Process
    response to hypoxia GO:0001666 IEA
    blood vessel remodeling GO:0001974 IEA
    response to ischemia GO:0002931 IEA
    mRNA splice site recognition GO:0006376 IDA
    RNA splicing GO:0008380 TAS
    regulation of gene expression GO:0010468 IEA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 IEA
    negative regulation of muscle atrophy GO:0014736 IEA
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 IDA
    response to injury involved in regulation of muscle adaptation GO:0014876 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    protein complex oligomerization GO:0051259 IDA
    negative regulation of programmed necrotic cell death GO:0062099 IEA
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IBA
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IDA
    intrinsic apoptotic signaling pathway GO:0097193 IEA
    regulation of non-canonical NF-kappaB signal transduction GO:1901222 IEA
    negative regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902109 IEA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IDA
    negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway GO:1903298 ISS
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IBA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IDA
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IBA
Subcellular Localization
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISS
    cytosol GO:0005829 TAS
    membrane GO:0016020 IEA
    sarcoplasmic reticulum GO:0016529 IEA
 Experiment description of studies that identified NOL3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NOL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AASDHPPT 60496
Co-fractionation Homo sapiens
2 EHD1 10938
Co-fractionation Homo sapiens
3 NAPRT 93100
Co-fractionation Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
5 CASP8 841
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
6 FADD 8772
Phenotypic Suppression Homo sapiens
7 GNPDA2 132789
Co-fractionation Homo sapiens
8 CFLAR  
Phenotypic Suppression Homo sapiens
9 NIF3L1 60491
Two-hybrid Homo sapiens
10 TNFRSF25  
Phenotypic Suppression Homo sapiens
11 POT1  
Two-hybrid Homo sapiens
12 EHD4 30844
Co-fractionation Homo sapiens
13 FAS 355
Phenotypic Suppression Homo sapiens
14 SRSF9 8683
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
15 UBQLN2 29978
Two-hybrid Homo sapiens
16 SIRPA 140885
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 STK39 27347
Co-fractionation Homo sapiens
18 TNFRSF1A 7132
Phenotypic Suppression Homo sapiens
19 CASP2  
Affinity Capture-Western Homo sapiens
20 TRADD 8717
Phenotypic Suppression Homo sapiens
21 FKBP9 11328
Co-fractionation Homo sapiens
22 TERF2IP 54386
Two-hybrid Homo sapiens
23 PACSIN3 29763
Affinity Capture-MS Homo sapiens
24 PNKP 11284
Affinity Capture-MS Homo sapiens
25 VASP 7408
Two-hybrid Homo sapiens
26 GTF3C4 9329
Co-fractionation Homo sapiens
27 APEX1 328
Affinity Capture-RNA Homo sapiens
28 PDCD10 11235
Co-fractionation Homo sapiens
29 TERF1 7013
Two-hybrid Homo sapiens
30 H2AFZ 3015
Co-fractionation Homo sapiens
31 NUBP1 4682
Co-fractionation Homo sapiens
32 ETNK2  
Two-hybrid Homo sapiens
33 UBA6 55236
Co-fractionation Homo sapiens
34 TINF2  
Two-hybrid Homo sapiens
35 NOL3 8996
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
36 DAXX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 XPNPEP1 7511
Co-fractionation Homo sapiens
38 PSMG1 8624
Co-fractionation Homo sapiens
39 IPO9 55705
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NOL3 is involved
No pathways found





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