Gene description for NOL3
Gene name nucleolar protein 3 (apoptosis repressor with CARD domain)
Gene symbol NOL3
Other names/aliases ARC
FCM
MYP
NOP
NOP30
Species Homo sapiens
 Database cross references - NOL3
ExoCarta ExoCarta_8996
Vesiclepedia VP_8996
Entrez Gene 8996
HGNC 7869
MIM 605235
UniProt O60936  
 NOL3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NOL3
Molecular Function
    caspase binding GO:0089720 IPI
    identical protein binding GO:0042802 IPI
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IDA
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IMP
    RNA binding GO:0003723 TAS
Biological Process
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IDA
    protein oligomerization GO:0051259 IDA
    RNA splicing GO:0008380 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IDA
    mRNA splice site selection GO:0006376 IDA
    response to hypoxia GO:0001666 IEA
    inhibition of cysteine-type endopeptidase activity involved in apoptotic process GO:1990001 IDA
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IDA
    response to injury involved in regulation of muscle adaptation GO:0014876 IEA
Subcellular Localization
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IDA
    sarcoplasmic reticulum GO:0016529 IEA
    cytosol GO:0005829 TAS
    mitochondrion GO:0005739 ISS
 Experiment description of studies that identified NOL3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NOL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRSF9 8683
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 SIRPA 140885
Two-hybrid Homo sapiens
3 CASP8 841
Invivo Homo sapiens
Invitro Homo sapiens
4 CASP2  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
5 NOL3 8996
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 NIF3L1 60491
Two-hybrid Homo sapiens
7 TFPT  
Affinity Capture-MS Homo sapiens
8 PACSIN3 29763
Affinity Capture-MS Homo sapiens
9 PNKP 11284
Affinity Capture-MS Homo sapiens
10 ETNK2  
Two-hybrid Homo sapiens
11 VASP 7408
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NOL3 is involved
No pathways found





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