Gene description for SIRPA
Gene name signal-regulatory protein alpha
Gene symbol SIRPA
Other names/aliases BIT
CD172A
MFR
MYD-1
P84
PTPNS1
SHPS1
SIRP
Species Homo sapiens
 Database cross references - SIRPA
ExoCarta ExoCarta_140885
Vesiclepedia VP_140885
Entrez Gene 140885
HGNC 9662
MIM 602461
UniProt P78324  
 SIRPA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for SIRPA
Molecular Function
    protein phosphatase inhibitor activity GO:0004864 ISS
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    protein phosphatase binding GO:0019903 ISS
    GTPase regulator activity GO:0030695 ISS
    protein binding involved in heterotypic cell-cell adhesion GO:0086080 ISS
    cell-cell adhesion mediator activity GO:0098632 IPI
    protein antigen binding GO:1990405 IPI
    protein tyrosine kinase binding GO:1990782 ISS
Biological Process
    cell adhesion GO:0007155 TAS
    regulation of gene expression GO:0010468 IMP
    cell migration GO:0016477 ISS
    negative regulation of lipopolysaccharide-mediated signaling pathway GO:0031665 ISS
    regulation of type II interferon production GO:0032649 IMP
    regulation of interleukin-1 beta production GO:0032651 ISS
    regulation of interleukin-6 production GO:0032675 ISS
    regulation of tumor necrosis factor production GO:0032680 IGI
    regulation of tumor necrosis factor production GO:0032680 ISS
    negative regulation of interferon-beta production GO:0032688 ISS
    negative regulation of interleukin-6 production GO:0032715 ISS
    negative regulation of tumor necrosis factor production GO:0032720 ISS
    heterotypic cell-cell adhesion GO:0034113 IEA
    monocyte extravasation GO:0035696 ISS
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISS
    negative regulation of nitric oxide biosynthetic process GO:0045019 ISS
    regulation of nitric oxide biosynthetic process GO:0045428 ISS
    negative regulation of JNK cascade GO:0046329 ISS
    negative regulation of inflammatory response GO:0050728 IMP
    negative regulation of phagocytosis GO:0050765 ISS
    positive regulation of phagocytosis GO:0050766 IBA
    positive regulation of T cell activation GO:0050870 IBA
    cellular response to hydrogen peroxide GO:0070301 ISS
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    cellular response to type II interferon GO:0071346 ISS
    cellular response to interleukin-1 GO:0071347 ISS
    cellular response to interleukin-12 GO:0071349 IMP
    negative regulation of macrophage inflammatory protein 1 alpha production GO:0071641 ISS
    negative regulation of chemokine (C-C motif) ligand 5 production GO:0071650 ISS
    negative regulation of cytokine production involved in inflammatory response GO:1900016 ISS
    positive regulation of reactive oxygen species metabolic process GO:2000379 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISS
    cell surface GO:0009986 TAS
    membrane GO:0016020 TAS
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified SIRPA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SIRPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX6 1656
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 CD47 961
Affinity Capture-MS Homo sapiens
3 TRIM2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 CALR 811
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 ACTN1 87
Reconstituted Complex Homo sapiens
6 NEXN 91624
Reconstituted Complex Homo sapiens
7 HSPA5 3309
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 TBX3 6926
Reconstituted Complex Homo sapiens
9 CLEC4A  
Affinity Capture-MS Homo sapiens
10 SMG7  
Reconstituted Complex Homo sapiens
11 MCAM 4162
Proximity Label-MS Homo sapiens
12 GRB2 2885
Affinity Capture-MS Homo sapiens
13 MATK  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 DYNLT1 6993
Two-hybrid Homo sapiens
15 STAT1 6772
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 HSPA8 3312
Reconstituted Complex Homo sapiens
17 PTPN6 5777
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
18 TPT1 7178
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 EIF4G1 1981
Cross-Linking-MS (XL-MS) Homo sapiens
20 VIM 7431
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 CCDC57 284001
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 SH2D1B  
Affinity Capture-MS Homo sapiens
23 KRT10 3858
Reconstituted Complex Homo sapiens
24 KRT34  
Two-hybrid Homo sapiens
25 CDK16 5127
Reconstituted Complex Homo sapiens
26 TCEB3 6924
Reconstituted Complex Homo sapiens
27 ARHGEF6 9459
Reconstituted Complex Homo sapiens
28 NOL3 8996
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 MX1 4599
Reconstituted Complex Homo sapiens
30 RPS8 6202
Reconstituted Complex Homo sapiens
31 SOS1 6654
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 HSP90AB1 3326
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 Htt  
Affinity Capture-MS Mus musculus
34 NOX4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 CAPZB 832
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 PFN1 5216
Reconstituted Complex Homo sapiens
37 FTH1 2495
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 GNL1 2794
Reconstituted Complex Homo sapiens
39 HSP90B1 7184
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
40 PHYH 5264
Reconstituted Complex Homo sapiens
41 NEK1  
Reconstituted Complex Homo sapiens
42 PTPN11 5781
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
43 ARF4 378
Reconstituted Complex Homo sapiens
44 HSF2BP  
Two-hybrid Homo sapiens
45 KRT15 3866
Two-hybrid Homo sapiens
46 ERGIC2 51290
Affinity Capture-MS Homo sapiens
47 CCT6A 908
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
48 CDH1 999
Proximity Label-MS Homo sapiens
49 PTPN7 5778
Reconstituted Complex Homo sapiens
50 FUBP1 8880
Reconstituted Complex Homo sapiens
51 TRIM23 373
Two-hybrid Homo sapiens
52 ND1  
Reconstituted Complex Homo sapiens
53 PPM1B 5495
Reconstituted Complex Homo sapiens
54 PSMA6 5687
Reconstituted Complex Homo sapiens
55 FLNA 2316
Reconstituted Complex Homo sapiens
56 TRIM27  
Two-hybrid Homo sapiens
57 COL6A2 1292
Reconstituted Complex Homo sapiens
58 HSPA1A 3303
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
59 HSPA4 3308
Reconstituted Complex Homo sapiens
60 CD81 975
Reconstituted Complex Homo sapiens
61 PSMC5 5705
Reconstituted Complex Homo sapiens
62 KRT40  
Two-hybrid Homo sapiens
63 PTPRA 5786
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 KRAS 3845
Proximity Label-MS Homo sapiens
65 KTN1 3895
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
66 ILK 3611
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
67 ANK3  
Cross-Linking-MS (XL-MS) Homo sapiens
68 SIRPB1 10326
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
69 KRT2 3849
Reconstituted Complex Homo sapiens
70 EIF5B 9669
Reconstituted Complex Homo sapiens
71 AKT1 207
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
72 HSPA9 3313
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
73 SUPT6H 6830
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
74 JAK2 3717
Affinity Capture-Western Homo sapiens
75 SAFB2 9667
Reconstituted Complex Homo sapiens
76 KRT31 3881
Two-hybrid Homo sapiens
77 SIRPG 55423
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 RHOB 388
Proximity Label-MS Homo sapiens
79 FAM114A2  
Affinity Capture-MS Homo sapiens
80 DDX10  
Reconstituted Complex Homo sapiens
81 NUCB1 4924
Reconstituted Complex Homo sapiens
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