Gene description for PHYH
Gene name phytanoyl-CoA 2-hydroxylase
Gene symbol PHYH
Other names/aliases LN1
LNAP1
PAHX
PHYH1
RD
Species Homo sapiens
 Database cross references - PHYH
ExoCarta ExoCarta_5264
Vesiclepedia VP_5264
Entrez Gene 5264
HGNC 8940
MIM 602026
UniProt O14832  
 PHYH identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PHYH
Molecular Function
    electron carrier activity GO:0009055 TAS
    L-ascorbic acid binding GO:0031418 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    cofactor binding GO:0048037 IDA
    phytanoyl-CoA dioxygenase activity GO:0048244 TAS
Biological Process
    cellular lipid metabolic process GO:0044255 TAS
    fatty acid alpha-oxidation GO:0001561 TAS
    isoprenoid metabolic process GO:0006720 IDA
    methyl-branched fatty acid metabolic process GO:0097089 IDA
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    peroxisome GO:0005777 IDA
    mitochondrion GO:0005739 IEA
    peroxisomal matrix GO:0005782 TAS
 Experiment description of studies that identified PHYH in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PHYH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WRAP73 49856
Affinity Capture-MS Homo sapiens
2 COPS3 8533
Affinity Capture-MS Homo sapiens
3 FKBP4 2288
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
4 TOM1L1 10040
Two-hybrid Homo sapiens
5 F8 2157
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
6 PEX7  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 PHYH 5264
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 MAGEA11  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PHYH is involved
PathwayEvidenceSource
Alpha-oxidation of phytanate TAS Reactome





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