Gene description for AGPS
Gene name alkylglycerone phosphate synthase
Gene symbol AGPS
Other names/aliases ADAP-S
ADAS
ADHAPS
ADPS
ALDHPSY
Species Homo sapiens
 Database cross references - AGPS
ExoCarta ExoCarta_8540
Vesiclepedia VP_8540
Entrez Gene 8540
HGNC 327
MIM 603051
UniProt O00116  
 AGPS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
 Gene ontology annotations for AGPS
Molecular Function
    protein binding GO:0005515 IPI
    alkylglycerone-phosphate synthase activity GO:0008609 IBA
    alkylglycerone-phosphate synthase activity GO:0008609 IDA
    FAD binding GO:0071949 ISS
Biological Process
    lipid biosynthetic process GO:0008610 IBA
    lipid biosynthetic process GO:0008610 IDA
    ether lipid biosynthetic process GO:0008611 IEA
    ether lipid biosynthetic process GO:0008611 ISS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal matrix GO:0005782 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified AGPS in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 248
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - non-transfected
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Lipids
Methods used in the study Western blotting
9
Experiment ID 249
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - transfected with alkylglycerone phosphate synthase siRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Lipids
Methods used in the study Western blotting
 Protein-protein interactions for AGPS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Co-fractionation Homo sapiens
2 FLOT2 2319
Proximity Label-MS Homo sapiens
3 DLG5 9231
Proximity Label-MS Homo sapiens
4 NDUFB8 4714
Co-fractionation Homo sapiens
5 Sgol2  
Affinity Capture-MS Mus musculus
6 DECR2 26063
Proximity Label-MS Homo sapiens
7 NOP58 51602
Affinity Capture-MS Homo sapiens
8 RAB1A 5861
Co-fractionation Homo sapiens
9 USP9X 8239
Proximity Label-MS Homo sapiens
10 SPAG5 10615
Proximity Label-MS Homo sapiens
11 PEX13  
Proximity Label-MS Homo sapiens
12 ALMS1  
Proximity Label-MS Homo sapiens
13 WNK1 65125
Proximity Label-MS Homo sapiens
14 KCTD10 83892
Affinity Capture-MS Homo sapiens
15 ACAA1 30
Proximity Label-MS Homo sapiens
16 TPP1 1200
Co-fractionation Homo sapiens
17 EPB41L5 57669
Proximity Label-MS Homo sapiens
18 HSDL2 84263
Proximity Label-MS Homo sapiens
19 CAT 847
Proximity Label-MS Homo sapiens
20 SMG7  
Proximity Label-MS Homo sapiens
21 APOE 348
Co-fractionation Homo sapiens
22 MRPL37 51253
Co-fractionation Homo sapiens
23 DVL3 1857
Proximity Label-MS Homo sapiens
24 PHYH 5264
Proximity Label-MS Homo sapiens
25 ANKRD28 23243
Proximity Label-MS Homo sapiens
26 CEP97 79598
Proximity Label-MS Homo sapiens
27 ABCD1 215
Co-fractionation Homo sapiens
28 KIAA1429 25962
Affinity Capture-MS Homo sapiens
29 POR 5447
Co-fractionation Homo sapiens
30 LONP2 83752
Proximity Label-MS Homo sapiens
31 MGST1 4257
Co-fractionation Homo sapiens
32 NDUFS3 4722
Co-fractionation Homo sapiens
33 PCNT  
Proximity Label-MS Homo sapiens
34 OGDH 4967
Co-fractionation Homo sapiens
35 PRDX3 10935
Co-fractionation Homo sapiens
36 KIF23 9493
Affinity Capture-MS Homo sapiens
37 MCM2 4171
Affinity Capture-MS Homo sapiens
38 CYB5R1 51706
Co-fractionation Homo sapiens
39 SDCCAG3  
Proximity Label-MS Homo sapiens
40 MRPS23 51649
Co-fractionation Homo sapiens
41 ERC1 23085
Proximity Label-MS Homo sapiens
42 ABCD3 5825
Co-fractionation Homo sapiens
43 ACOT8 10005
Proximity Label-MS Homo sapiens
44 ATP5O 539
Co-fractionation Homo sapiens
45 Cdca5  
Affinity Capture-MS Mus musculus
46 CHORDC1 26973
Affinity Capture-MS Homo sapiens
47 ATG16L1 55054
Affinity Capture-MS Homo sapiens
48 MECP2 4204
Affinity Capture-MS Homo sapiens
49 HACL1 26061
Proximity Label-MS Homo sapiens
50 HSD17B4 3295
Proximity Label-MS Homo sapiens
51 DVL2 1856
Proximity Label-MS Homo sapiens
52 CEP131 22994
Proximity Label-MS Homo sapiens
53 VWA8 23078
Proximity Label-MS Homo sapiens
54 GNPAT 8443
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
55 TMEM87A 25963
Co-fractionation Homo sapiens
56 HSPA8 3312
Affinity Capture-MS Homo sapiens
57 LMAN1 3998
Co-fractionation Homo sapiens
58 GOLGA2 2801
Proximity Label-MS Homo sapiens
59 NNT 23530
Co-fractionation Homo sapiens
60 PTGES2 80142
Co-fractionation Homo sapiens
61 ATP5A1 498
Co-fractionation Homo sapiens
62 P4HA1 5033
Co-fractionation Homo sapiens
63 ACOT9 23597
Co-fractionation Homo sapiens
64 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CEP192 55125
Proximity Label-MS Homo sapiens
66 NDUFA8 4702
Co-fractionation Homo sapiens
67 WRAP73 49856
Proximity Label-MS Homo sapiens
68 TUFM 7284
Co-fractionation Homo sapiens
69 FAM193A  
Proximity Label-MS Homo sapiens
70 CEP152  
Proximity Label-MS Homo sapiens
71 CHMP4B 128866
Affinity Capture-MS Homo sapiens
72 NTRK1 4914
Affinity Capture-MS Homo sapiens
73 SAR1B 51128
Affinity Capture-MS Homo sapiens
74 IQCB1  
Proximity Label-MS Homo sapiens
75 PEX14 5195
Co-purification Homo sapiens
Proximity Label-MS Homo sapiens
76 CCDC8  
Affinity Capture-MS Homo sapiens
77 UQCRC1 7384
Co-fractionation Homo sapiens
78 ACTB 60
Co-fractionation Homo sapiens
79 IDH3G 3421
Co-fractionation Homo sapiens
80 ACOX1 51
Proximity Label-MS Homo sapiens
81 GNG5 2787
Co-fractionation Homo sapiens
82 NIPSNAP1 8508
Co-fractionation Homo sapiens
83 ATP5B 506
Co-fractionation Homo sapiens
84 ANLN 54443
Affinity Capture-MS Homo sapiens
85 N4BP2  
Proximity Label-MS Homo sapiens
86 PHB 5245
Co-fractionation Homo sapiens
87 ECH1 1891
Proximity Label-MS Homo sapiens
88 HADHB 3032
Co-fractionation Homo sapiens
89 HADHA 3030
Co-fractionation Homo sapiens
90 CANX 821
Co-fractionation Homo sapiens
91 NUDT12  
Proximity Label-MS Homo sapiens
92 NDUFB10 4716
Co-fractionation Homo sapiens
93 EMC8 10328
Co-fractionation Homo sapiens
94 LRRC59 55379
Co-fractionation Homo sapiens
95 ECT2 1894
Affinity Capture-MS Homo sapiens
96 LETM1 3954
Co-fractionation Homo sapiens
97 UQCRC2 7385
Co-fractionation Homo sapiens
98 HMOX2 3163
Co-fractionation Homo sapiens
99 EEF2 1938
Co-fractionation Homo sapiens
100 ACAD11 84129
Proximity Label-MS Homo sapiens
101 TYSND1  
Proximity Label-MS Homo sapiens
102 RIPK4  
Affinity Capture-MS Homo sapiens
103 NCDN 23154
Affinity Capture-MS Homo sapiens
104 CDK5RAP2 55755
Proximity Label-MS Homo sapiens
105 ECI2 10455
Proximity Label-MS Homo sapiens
106 TOMM6  
Co-fractionation Homo sapiens
107 RPA3 6119
Proximity Label-MS Homo sapiens
108 Sept6  
Affinity Capture-MS Mus musculus
109 EMC2 9694
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
110 GSTK1 373156
Proximity Label-MS Homo sapiens
111 SIRT2 22933
Proximity Label-MS Homo sapiens
112 PREB 10113
Co-fractionation Homo sapiens
113 CSRP2 1466
Co-fractionation Homo sapiens
114 NDUFA3  
Co-fractionation Homo sapiens
115 CCDC88A 55704
Proximity Label-MS Homo sapiens
116 CORO1C 23603
Co-fractionation Homo sapiens
117 MDM2  
Affinity Capture-MS Homo sapiens
118 SCCPDH 51097
Co-fractionation Homo sapiens
119 HUWE1 10075
Affinity Capture-MS Homo sapiens
120 MPHOSPH9  
Proximity Label-MS Homo sapiens
121 DENND4C 55667
Proximity Label-MS Homo sapiens
122 SLC25A3 5250
Co-fractionation Homo sapiens
123 DVL1 1855
Proximity Label-MS Homo sapiens
124 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
125 PHB2 11331
Co-fractionation Homo sapiens
126 SIRT6  
Affinity Capture-MS Homo sapiens
127 RBM11  
Proximity Label-MS Homo sapiens
128 BKRF1  
Affinity Capture-MS
129 TTC28 23331
Proximity Label-MS Homo sapiens
130 WWOX 51741
Proximity Label-MS Homo sapiens
131 DNAJA2 10294
Proximity Label-MS Homo sapiens
132 LONP1 9361
Co-fractionation Homo sapiens
133 EHHADH 1962
Proximity Label-MS Homo sapiens
134 LGALS3 3958
Co-fractionation Homo sapiens
135 BCKDHA 593
Co-fractionation Homo sapiens
136 PDHA1 5160
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
137 NDUFS7 374291
Co-fractionation Homo sapiens
138 PDHB 5162
Co-fractionation Homo sapiens
139 NUDT19 390916
Proximity Label-MS Homo sapiens
140 DNMBP 23268
Proximity Label-MS Homo sapiens
141 SIRT1  
Proximity Label-MS Homo sapiens
142 PRRC2B  
Proximity Label-MS Homo sapiens
143 GORASP1 64689
Two-hybrid Homo sapiens
144 SFXN1 94081
Co-fractionation Homo sapiens
145 PNPLA8 50640
Proximity Label-MS Homo sapiens
146 STRN4 29888
Proximity Label-MS Homo sapiens
147 PARD6B 84612
Affinity Capture-MS Homo sapiens
148 RAB7A 7879
Co-fractionation Homo sapiens
149 CYB5R3 1727
Co-fractionation Homo sapiens
150 DHRS4 10901
Proximity Label-MS Homo sapiens
151 EPHA2 1969
Proximity Label-MS Homo sapiens
152 PEX5 5830
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
153 ATP5C1 509
Co-fractionation Homo sapiens
154 LOX 4015
Co-fractionation Homo sapiens
155 DSG2 1829
Proximity Label-MS Homo sapiens
156 SCP2 6342
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
157 KIF1C 10749
Proximity Label-MS Homo sapiens
158 KIAA0355 9710
Proximity Label-MS Homo sapiens
159 EP300 2033
Affinity Capture-MS Homo sapiens
160 NYNRIN  
Proximity Label-MS Homo sapiens
161 CNTROB  
Proximity Label-MS Homo sapiens
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