Gene description for PHB
Gene name prohibitin
Gene symbol PHB
Other names/aliases HEL-215
HEL-S-54e
PHB1
Species Homo sapiens
 Database cross references - PHB
ExoCarta ExoCarta_5245
Entrez Gene 5245
HGNC 8912
MIM 176705
UniProt P35232  
 PHB identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Breast cancer cells 19415654    
Colorectal cancer cells 17956143    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PHB
Molecular Function
    transcription regulatory region DNA binding GO:0044212 IDA
    complement component C3b binding GO:0001851 IDA
    proteinase activated receptor binding GO:0031871 IPI
    histone deacetylase binding GO:0042826 IPI
    enzyme binding GO:0019899 IPI
    sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0000981 IEA
    protein binding GO:0005515 IPI
    protein C-terminus binding GO:0008022 IPI
    complement component C3a binding GO:0001850 IDA
Biological Process
    DNA biosynthetic process GO:0071897 IEA
    mitochondrion organization GO:0007005 IMP
    negative regulation of glucocorticoid receptor signaling pathway GO:2000323 IDA
    negative regulation of androgen receptor signaling pathway GO:0060766 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    negative regulation of protein catabolic process GO:0042177 IMP
    negative regulation of transcription by competitive promoter binding GO:0010944 IDA
    progesterone receptor signaling pathway GO:0050847 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    osteoblast differentiation GO:0001649 IDA
    histone deacetylation GO:0016575 IDA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IMP
    regulation of apoptotic process GO:0042981 TAS
    negative regulation of cell proliferation GO:0008285 IMP
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of cell growth GO:0030308 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    protein stabilization GO:0050821 IMP
    signal transduction GO:0007165 TAS
    transcription from RNA polymerase II promoter GO:0006366 IEA
    positive regulation of complement activation GO:0045917 IDA
    positive regulation of G-protein coupled receptor protein signaling pathway GO:0045745 IMP
    cellular response to interleukin-6 GO:0071354 IDA
    regulation of transcription, DNA-templated GO:0006355 IDA
Subcellular Localization
    cell surface GO:0009986 IDA
    early endosome GO:0005769 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 IDA
    integral component of plasma membrane GO:0005887 IDA
    nucleus GO:0005634 TAS
    plasma membrane GO:0005886 IDA
    mitochondrial inner membrane GO:0005743 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified PHB in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 65
ISEV standards
EV Biophysical techniques
HSP60|HSP27|GAPDH
EV Cytosolic markers
FLOT2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors Staubach S, Razawi H, Hanisch FG.
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
4
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RB1 5925
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 RBL1  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 RAF1 5894
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 MAP3K10  
Invitro Homo sapiens
5 E2F1 1869
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
6 SMARCA2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 ACTN1 87
Affinity Capture-Western Homo sapiens
8 ANXA2 302
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 NCOR1  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
10 SMARCA4 6597
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
12 SIN3A  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
13 RBL2 5934
Invivo Homo sapiens
Invitro Homo sapiens
14 TP53  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
View the network image/svg+xml
 Pathways in which PHB is involved
No pathways found





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