Gene description for PAF1
Gene name Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
Gene symbol PAF1
Other names/aliases F23149_1
PD2
Species Homo sapiens
 Database cross references - PAF1
ExoCarta ExoCarta_54623
Vesiclepedia VP_54623
Entrez Gene 54623
HGNC 25459
MIM 610506
UniProt Q8N7H5  
 PAF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PAF1
Molecular Function
    RNA polymerase II complex binding GO:0000993 IBA
    RNA polymerase II complex binding GO:0000993 IDA
    chromatin binding GO:0003682 IBA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    endodermal cell fate commitment GO:0001711 ISS
    transcription elongation by RNA polymerase II GO:0006368 IDA
    Wnt signaling pathway GO:0016055 IEA
    stem cell population maintenance GO:0019827 IEA
    mRNA 3'-end processing GO:0031124 IMP
    protein localization to nucleus GO:0034504 IMP
    negative regulation of myeloid cell differentiation GO:0045638 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    cellular response to lipopolysaccharide GO:0071222 ISS
    positive regulation of cell cycle G1/S phase transition GO:1902808 IMP
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 IDA
    Cdc73/Paf1 complex GO:0016593 IBA
    Cdc73/Paf1 complex GO:0016593 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified PAF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 SNRPA 6626
Affinity Capture-MS Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 CPSF6 11052
Affinity Capture-MS Homo sapiens
7 SENP3 26168
Affinity Capture-MS Homo sapiens
8 FUS 2521
Affinity Capture-MS Homo sapiens
9 MYLIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COIL  
Proximity Label-MS Homo sapiens
11 SKIV2L 6499
Affinity Capture-MS Homo sapiens
12 SSB 6741
Affinity Capture-MS Homo sapiens
13 NOP56 10528
Proximity Label-MS Homo sapiens
14 DHX8 1659
Proximity Label-MS Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 CSNK2A1 1457
Co-fractionation Homo sapiens
17 SNRPF 6636
Affinity Capture-MS Homo sapiens
18 RNF40 9810
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
19 SERINC2 347735
Affinity Capture-MS Homo sapiens
20 APEX1 328
Proximity Label-MS Homo sapiens
21 TCEB3 6924
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 DDX23 9416
Proximity Label-MS Homo sapiens
23 PC 5091
Affinity Capture-MS Homo sapiens
24 CHAF1A  
Affinity Capture-MS Homo sapiens
25 CDK11B 984
Co-fractionation Homo sapiens
26 FBXW7  
Affinity Capture-MS Homo sapiens
27 MDC1  
Affinity Capture-MS Homo sapiens
28 SNRPC 6631
Affinity Capture-MS Homo sapiens
29 POLR1E  
Proximity Label-MS Homo sapiens
30 SYT2  
Affinity Capture-MS Homo sapiens
31 SLX4  
Affinity Capture-MS Homo sapiens
32 LEO1 123169
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 MSL2  
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
34 PHB 5245
Affinity Capture-MS Homo sapiens
35 TULP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ARFGEF2 10564
Co-fractionation Homo sapiens
37 MLLT1 4298
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 CSNK2B 1460
Affinity Capture-MS Homo sapiens
39 HSPA14 51182
Co-fractionation Homo sapiens
40 YAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MSL3  
Reconstituted Complex Homo sapiens
42 CHD1 1105
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
43 TTC37 9652
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 SRP9 6726
Affinity Capture-MS Homo sapiens
45 COX7A2 1347
Affinity Capture-MS Homo sapiens
46 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
47 SRRM1 10250
Co-fractionation Homo sapiens
48 PARK2  
Affinity Capture-MS Homo sapiens
49 SUPT5H 6829
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 WDR61 80349
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
51 DNMT3A 1788
Affinity Capture-MS Homo sapiens
52 RTF1 23168
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
53 ACACA 31
Affinity Capture-MS Homo sapiens
54 RCCD1  
Affinity Capture-MS Homo sapiens
55 PCCA 5095
Affinity Capture-MS Homo sapiens
56 Wdr61  
Affinity Capture-MS Mus musculus
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 RPL31 6160
Proximity Label-MS Homo sapiens
59 HIST1H1C 3006
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
60 RPS6 6194
Affinity Capture-MS Homo sapiens
61 CTR9 9646
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
62 MCCC1 56922
Affinity Capture-MS Homo sapiens
63 ARL4C 10123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 Ksr1  
Affinity Capture-MS Mus musculus
65 HIST1H2BJ 8970
Affinity Capture-MS Homo sapiens
66 CD3EAP  
Proximity Label-MS Homo sapiens
67 ECH1 1891
Affinity Capture-MS Homo sapiens
68 PTPN11 5781
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
69 PSMA3 5684
Affinity Capture-MS Homo sapiens
70 HIST1H3A 8350
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
71 KMT2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
72 CDC73  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
73 MCCC2 64087
Affinity Capture-MS Homo sapiens
74 CIAO1 9391
Affinity Capture-MS Homo sapiens
75 XPO1 7514
Affinity Capture-MS Homo sapiens
76 SNRPB 6628
Affinity Capture-MS Homo sapiens
77 HTATSF1 27336
Co-fractionation Homo sapiens
78 MYH14 79784
Co-fractionation Homo sapiens
79 CIT 11113
Affinity Capture-MS Homo sapiens
80 UBE2E1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
81 DCTN3 11258
Affinity Capture-MS Homo sapiens
82 ATP1B3 483
Affinity Capture-MS Homo sapiens
83 METTL21B  
Affinity Capture-MS Homo sapiens
84 POLR2A 5430
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
85 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
86 AGO4  
Affinity Capture-MS Homo sapiens
87 DNMT3B  
Affinity Capture-MS Homo sapiens
88 RPA3 6119
Proximity Label-MS Homo sapiens
89 FXR2 9513
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 MSL1 339287
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
91 CSNK2A2 1459
Co-fractionation Homo sapiens
92 TOP1 7150
Affinity Capture-MS Homo sapiens
93 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
94 KAT8  
Reconstituted Complex Homo sapiens
95 VDAC1 7416
Affinity Capture-MS Homo sapiens
96 CDC7  
Co-fractionation Homo sapiens
97 NEUROG3  
Affinity Capture-MS Homo sapiens
98 CHUK 1147
Co-fractionation Homo sapiens
99 RIPK4  
Affinity Capture-MS Homo sapiens
100 PXK 54899
Affinity Capture-MS Homo sapiens
101 SUPT16H 11198
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
102 DNAJC6  
Affinity Capture-MS Homo sapiens
103 XRCC5 7520
Co-fractionation Homo sapiens
104 ZRANB2 9406
Co-fractionation Homo sapiens
105 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
106 POLR2F  
Affinity Capture-MS Homo sapiens
107 PARP1 142
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
108 ILF3 3609
Affinity Capture-RNA Homo sapiens
109 TTC33  
Affinity Capture-MS Homo sapiens
110 ATP5J 522
Affinity Capture-MS Homo sapiens
111 PGRMC1 10857
Affinity Capture-MS Homo sapiens
112 TCEA1 6917
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
113 MEN1 4221
Affinity Capture-MS Homo sapiens
114 COX5A 9377
Affinity Capture-MS Homo sapiens
115 ZC3H4 23211
Co-fractionation Homo sapiens
116 HSPB1 3315
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
117 RNF20 56254
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
118 NUP98 4928
Co-fractionation Homo sapiens
119 CHD2 1106
Co-fractionation Homo sapiens
120 AP3B1 8546
Co-fractionation Homo sapiens
121 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
122 Tox4  
Affinity Capture-MS Mus musculus
123 Rprd1b  
Affinity Capture-MS Mus musculus
124 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
125 CDK9 1025
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
126 PCCB 5096
Affinity Capture-MS Homo sapiens
127 CYB5R3 1727
Affinity Capture-MS Homo sapiens
128 SGOL1  
Affinity Capture-MS Homo sapiens
129 MLLT3 4300
Affinity Capture-Western Homo sapiens
130 SERBP1 26135
Affinity Capture-MS Homo sapiens
131 KRAS 3845
Synthetic Lethality Homo sapiens
132 C9orf72  
Affinity Capture-MS Homo sapiens
133 SSRP1 6749
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
134 CAB39L 81617
Affinity Capture-MS Homo sapiens
135 EP300 2033
Affinity Capture-MS Homo sapiens
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