Gene description for WDR61
Gene name WD repeat domain 61
Gene symbol WDR61
Other names/aliases REC14
SKI8
Species Homo sapiens
 Database cross references - WDR61
ExoCarta ExoCarta_80349
Entrez Gene 80349
HGNC 30300
MIM 609540
UniProt Q9GZS3  
 WDR61 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for WDR61
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of histone H3-K4 methylation GO:0051571 IMP
    positive regulation of transcription elongation from RNA polymerase II promoter GO:0032968 IDA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of histone H3-K79 methylation GO:2001162 IMP
    negative regulation of myeloid cell differentiation GO:0045638 IDA
    nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' GO:0034427 IBA
    histone H3-K4 trimethylation GO:0080182 IMP
    Wnt signaling pathway GO:0016055 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
Subcellular Localization
    Cdc73/Paf1 complex GO:0016593 IDA
    Ski complex GO:0055087 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    transcriptionally active chromatin GO:0035327 IDA
 Experiment description of studies that identified WDR61 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR61
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EZH2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WDR61 is involved
No pathways found





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