Gene description for SNRPB
Gene name small nuclear ribonucleoprotein polypeptides B and B1
Gene symbol SNRPB
Other names/aliases CCMS
COD
SNRPB1
Sm-B/B'
SmB/B'
SmB/SmB'
snRNP-B
Species Homo sapiens
 Database cross references - SNRPB
ExoCarta ExoCarta_6628
Entrez Gene 6628
HGNC 11153
MIM 182282
UniProt P14678  
 SNRPB identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for SNRPB
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    histone pre-mRNA DCP binding GO:0071208 IEA
Biological Process
    mRNA 3'-end processing GO:0031124 TAS
    ncRNA metabolic process GO:0034660 TAS
    positive regulation of defense response to virus by host GO:0002230 IMP
    protein methylation GO:0006479 IDA
    RNA splicing GO:0008380 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    spliceosomal snRNP assembly GO:0000387 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    mRNA splicing, via spliceosome GO:0000398 TAS
    gene expression GO:0010467 TAS
    histone mRNA metabolic process GO:0008334 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    U7 snRNP GO:0005683 IDA
    cytosol GO:0005829 TAS
    histone pre-mRNA 3'end processing complex GO:0071204 IEA
    extracellular exosome GO:0070062 IDA
    small nuclear ribonucleoprotein complex GO:0030532 TAS
    U2 snRNP GO:0005686 IBA
    U5 snRNP GO:0005682 IBA
    U12-type spliceosomal complex GO:0005689 IDA
    U1 snRNP GO:0005685 IDA
    methylosome GO:0034709 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U2-type prespliceosome GO:0071004 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
    nucleoplasm GO:0005654 TAS
    SMN-Sm protein complex GO:0034719 IDA
    spliceosomal complex GO:0005681 TAS
    U4 snRNP GO:0005687 IDA
 Experiment description of studies that identified SNRPB in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SNRPB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SART3 9733
Affinity Capture-MS Homo sapiens
2 HTATSF1 27336
Affinity Capture-MS Homo sapiens
3 ASCC2  
Two-hybrid Homo sapiens
4 SMN1 6606
Invitro Homo sapiens
5 SNRNP200 23020
Affinity Capture-MS Homo sapiens
6 GEMIN4 50628
Affinity Capture-MS Homo sapiens
7 EFTUD2 9343
Affinity Capture-MS Homo sapiens
8 KHK 3795
Affinity Capture-MS Homo sapiens
9 PSMA3 5684
Two-hybrid Homo sapiens
10 LGALS1 3956
Affinity Capture-Western Homo sapiens
11 TOP3B  
Two-hybrid Homo sapiens
12 COIL  
Invitro Homo sapiens
13 CTDP1  
Invivo Homo sapiens
14 DDX20  
Invivo Homo sapiens
Invitro Homo sapiens
15 C14orf1 11161
Two-hybrid Homo sapiens
16 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
17 PRPF8 10594
Affinity Capture-MS Homo sapiens
18 SNRPD3 6634
Invitro Homo sapiens
19 CLNS1A 1207
Affinity Capture-Western Homo sapiens
20 WDR77 79084
Reconstituted Complex Homo sapiens
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