Gene description for CWC27
Gene name CWC27 spliceosome-associated protein homolog (S. cerevisiae)
Gene symbol CWC27
Other names/aliases NY-CO-10
SDCCAG10
Species Homo sapiens
 Database cross references - CWC27
ExoCarta ExoCarta_10283
Vesiclepedia VP_10283
Entrez Gene 10283
HGNC 10664
UniProt Q6UX04  
 CWC27 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for CWC27
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IC
    protein folding GO:0006457 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    U2-type precatalytic spliceosome GO:0071005 IDA
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IDA
 Experiment description of studies that identified CWC27 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for CWC27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 EPB41L1 2036
Affinity Capture-MS Homo sapiens
5 PARP1 142
Proximity Label-MS Homo sapiens
6 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 C9orf78 51759
Affinity Capture-MS Homo sapiens
8 LMNB1 4001
Proximity Label-MS Homo sapiens
9 SCYL1 57410
Co-fractionation Homo sapiens
10 QARS 5859
Cross-Linking-MS (XL-MS) Homo sapiens
11 XRCC4  
Co-fractionation Homo sapiens
12 EPB41L3 23136
Affinity Capture-MS Homo sapiens
13 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
14 EPB41L5 57669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PLCD3 113026
Affinity Capture-MS Homo sapiens
16 DHX8 1659
Proximity Label-MS Homo sapiens
17 YWHAZ 7534
Affinity Capture-MS Homo sapiens
18 HOXC10  
Co-fractionation Homo sapiens
19 MORF4L1  
Two-hybrid Homo sapiens
20 SIPA1L1 26037
Co-fractionation Homo sapiens
21 PRKDC 5591
Co-fractionation Homo sapiens
22 CBX3 11335
Proximity Label-MS Homo sapiens
23 DDX23 9416
Proximity Label-MS Homo sapiens
24 SKIV2L2 23517
Co-fractionation Homo sapiens
25 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SNRPB 6628
Cross-Linking-MS (XL-MS) Homo sapiens
27 WDR76  
Affinity Capture-MS Homo sapiens
28 IGSF6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 YAP1 10413
Affinity Capture-MS Homo sapiens
30 PDCD5 9141
Co-fractionation Homo sapiens
31 OGT 8473
Reconstituted Complex Homo sapiens
32 LSM6 11157
Co-fractionation Homo sapiens
33 FRA10AC1 118924
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
34 XRCC6 2547
Proximity Label-MS Homo sapiens
35 ARRDC3 57561
Affinity Capture-MS Homo sapiens
36 SNW1 22938
Co-fractionation Homo sapiens
37 LSM2 57819
Co-fractionation Homo sapiens
38 SYT2  
Affinity Capture-MS Homo sapiens
39 LMNA 4000
Proximity Label-MS Homo sapiens
40 DHX38 9785
Co-fractionation Homo sapiens
41 RPA3 6119
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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