Gene description for SCYL1
Gene name SCY1-like 1 (S. cerevisiae)
Gene symbol SCYL1
Other names/aliases GKLP
HT019
NKTL
NTKL
P105
TAPK
TEIF
TRAP
Species Homo sapiens
 Database cross references - SCYL1
ExoCarta ExoCarta_57410
Vesiclepedia VP_57410
Entrez Gene 57410
HGNC 14372
MIM 607982
UniProt Q96KG9  
 SCYL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SCYL1
Molecular Function
    DNA binding GO:0003677 IEA
    protein tyrosine kinase activity GO:0004713 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cadherin binding GO:0045296 HDA
Biological Process
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IDA
    inflammatory response GO:0006954 IEA
    protein localization GO:0008104 IEA
    spinal cord motor neuron differentiation GO:0021522 IEA
    neuron development GO:0048666 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    Golgi apparatus GO:0005794 IDA
    cis-Golgi network GO:0005801 IDA
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    COPI vesicle coat GO:0030126 IDA
 Experiment description of studies that identified SCYL1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SCYL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCNF  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 DDX6 1656
Two-hybrid Homo sapiens
3 CDC25A  
Two-hybrid Homo sapiens
4 LMAN2 10960
Affinity Capture-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 TMED10 10972
Affinity Capture-MS Homo sapiens
8 COPZ1 22818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 EBAG9 9166
Proximity Label-MS Homo sapiens
10 SLU7  
Affinity Capture-MS Homo sapiens
11 COIL  
Two-hybrid Homo sapiens
12 SLC35G1  
Affinity Capture-MS Homo sapiens
13 AVIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CWC27 10283
Co-fractionation Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 GJA1 2697
Proximity Label-MS Homo sapiens
17 NUP155 9631
Proximity Label-MS Homo sapiens
18 GARS 2617
Affinity Capture-MS Homo sapiens
19 PIN1 5300
Two-hybrid Homo sapiens
20 AURKA 6790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 P4HA2 8974
Affinity Capture-MS Homo sapiens
22 UBR3  
Affinity Capture-MS Homo sapiens
23 SRSF1 6426
Affinity Capture-MS Homo sapiens
24 MEN1 4221
Affinity Capture-MS Homo sapiens
25 NEK7 140609
Affinity Capture-MS Homo sapiens
26 EMD 2010
Proximity Label-MS Homo sapiens
27 PLEKHF2 79666
Two-hybrid Homo sapiens
28 SMURF1 57154
Biochemical Activity Homo sapiens
29 IKBIP 121457
Affinity Capture-MS Homo sapiens
30 ACTR1B 10120
Co-fractionation Homo sapiens
31 EGFR 1956
Affinity Capture-MS Homo sapiens
32 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
33 CHD1L 9557
Two-hybrid Homo sapiens
34 AGPAT3 56894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 COPE 11316
Affinity Capture-MS Homo sapiens
36 DNAJB3 414061
Affinity Capture-MS Homo sapiens
37 DDX58 23586
Affinity Capture-RNA Homo sapiens
38 COPG1 22820
Affinity Capture-MS Homo sapiens
39 SPTY2D1  
Co-fractionation Homo sapiens
40 APP 351
Reconstituted Complex Homo sapiens
41 APOD 347
Affinity Capture-MS Homo sapiens
42 CAPZB 832
Affinity Capture-MS Homo sapiens
43 ARCN1 372
Affinity Capture-MS Homo sapiens
44 POLA2  
Co-fractionation Homo sapiens
45 COPB1 1315
Affinity Capture-MS Homo sapiens
46 GORAB 92344
Two-hybrid Homo sapiens
47 MEIS2  
Affinity Capture-MS Homo sapiens
48 BTBD2  
Affinity Capture-MS Homo sapiens
49 PARK2  
Affinity Capture-MS Homo sapiens
50 AKR7L 246181
Affinity Capture-MS Homo sapiens
51 CDH1 999
Proximity Label-MS Homo sapiens
52 EFCAB1  
Affinity Capture-MS Homo sapiens
53 MYC  
Dosage Lethality Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 SEC31A 22872
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 MCM2 4171
Co-fractionation Homo sapiens
58 Rhoa 11848
Affinity Capture-MS Mus musculus
59 CTDSPL2  
Affinity Capture-MS Homo sapiens
60 COPB2 9276
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
61 LENG1  
Two-hybrid Homo sapiens
62 SEC24C 9632
Affinity Capture-MS Homo sapiens
63 CPSF6 11052
Affinity Capture-MS Homo sapiens
64 LUC7L2 51631
Affinity Capture-MS Homo sapiens
65 EP300 2033
Affinity Capture-MS Homo sapiens
66 SORT1 6272
Affinity Capture-MS Homo sapiens
67 CRTC3  
Co-fractionation Homo sapiens
68 COPA 1314
Affinity Capture-MS Homo sapiens
69 CKAP4 10970
Proximity Label-MS Homo sapiens
70 TCEA2  
Two-hybrid Homo sapiens
71 Slc6a8 102857
Affinity Capture-MS Mus musculus
72 REST 5978
Affinity Capture-Western Homo sapiens
73 DUSP23 54935
Affinity Capture-MS Homo sapiens
74 ARID1A 8289
Co-fractionation Homo sapiens
75 ALS2CR11  
Affinity Capture-MS Homo sapiens
76 CES2 8824
Affinity Capture-MS Homo sapiens
77 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 SATB1 6304
Affinity Capture-MS Homo sapiens
79 SLC25A46 91137
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SCYL1 is involved
No pathways found





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