Gene description for SCYL1
Gene name SCY1-like 1 (S. cerevisiae)
Gene symbol SCYL1
Other names/aliases GKLP
HT019
NKTL
NTKL
P105
TAPK
TEIF
TRAP
Species Homo sapiens
 Database cross references - SCYL1
ExoCarta ExoCarta_57410
Entrez Gene 57410
HGNC 14372
MIM 607982
UniProt Q96KG9  
 SCYL1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for SCYL1
Molecular Function
    DNA binding GO:0003677 IEA
    protein tyrosine kinase activity GO:0004713 NAS
    ATP binding GO:0005524 IEA
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    retrograde vesicle-mediated transport, Golgi to ER GO:0006890 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 NAS
    regulation of transcription, DNA-templated GO:0006355 IEA
Subcellular Localization
    microtubule organizing center GO:0005815 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IDA
    cytoplasm GO:0005737 ISS
    nucleus GO:0005634 IEA
    Golgi apparatus GO:0005794 IDA
    cis-Golgi network GO:0005801 IDA
    membrane GO:0016020 IDA
    COPI vesicle coat GO:0030126 IDA
 Experiment description of studies that identified SCYL1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SCYL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SCYL1 is involved
No pathways found





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