Gene description for SEC31A
Gene name SEC31 homolog A (S. cerevisiae)
Gene symbol SEC31A
Other names/aliases ABP125
ABP130
HSPC275
HSPC334
SEC31L1
Species Homo sapiens
 Database cross references - SEC31A
ExoCarta ExoCarta_22872
Entrez Gene 22872
HGNC 17052
MIM 610257
UniProt O94979  
 SEC31A identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 15326289    
 Gene ontology annotations for SEC31A
Molecular Function
    protein binding GO:0005515 IPI
    calcium-dependent protein binding GO:0048306 IPI
Biological Process
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    ER to Golgi vesicle-mediated transport GO:0006888 TAS
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    protein transport GO:0015031 IEA
    IRE1-mediated unfolded protein response GO:0036498 TAS
    post-translational protein modification GO:0043687 TAS
    COPII vesicle coating GO:0048208 TAS
    response to calcium ion GO:0051592 IDA
    protein N-linked glycosylation via asparagine GO:0018279 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    membrane organization GO:0061024 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    Golgi membrane GO:0000139 TAS
    endoplasmic reticulum GO:0005783 IDA
    ER to Golgi transport vesicle GO:0030134 IDA
    endoplasmic reticulum exit site GO:0070971 IMP
    COPII vesicle coat GO:0030127 NAS
    vesicle coat GO:0030120 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified SEC31A in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for SEC31A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC13 6396
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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