Gene description for CTH
Gene name cystathionine gamma-lyase
Gene symbol CTH
Other names/aliases -
Species Homo sapiens
 Database cross references - CTH
ExoCarta ExoCarta_1491
Vesiclepedia VP_1491
Entrez Gene 1491
HGNC 2501
MIM 607657
UniProt P32929  
 CTH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for CTH
Molecular Function
    cystathionine gamma-lyase activity GO:0004123 IBA
    cystathionine gamma-lyase activity GO:0004123 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    pyridoxal phosphate binding GO:0030170 IBA
    pyridoxal phosphate binding GO:0030170 IDA
    identical protein binding GO:0042802 IPI
    L-cystine L-cysteine-lyase (deaminating) GO:0044540 IMP
    homocysteine desulfhydrase activity GO:0047982 IEA
    L-cysteine desulfhydrase activity GO:0080146 IEA
    selenocystathionine gamma-lyase activity GO:0098606 IEA
Biological Process
    cysteine metabolic process GO:0006534 TAS
    lipid metabolic process GO:0006629 IEA
    protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine GO:0018272 IDA
    cysteine biosynthetic process via cystathionine GO:0019343 IBA
    cysteine biosynthetic process via cystathionine GO:0019343 IDA
    cysteine biosynthetic process GO:0019344 IDA
    transsulfuration GO:0019346 IBA
    transsulfuration GO:0019346 IDA
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEA
    protein sulfhydration GO:0044524 IMP
    protein homotetramerization GO:0051289 IPI
    hydrogen sulfide biosynthetic process GO:0070814 IDA
    hydrogen sulfide biosynthetic process GO:0070814 TAS
    positive regulation of aortic smooth muscle cell differentiation GO:1904831 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEA
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CTH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CTH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 ENO2 2026
Co-fractionation Homo sapiens
3 RPA2 6118
Proximity Label-MS Homo sapiens
4 KLHL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GUCD1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 NUTF2 10204
Co-fractionation Homo sapiens
7 SPRY2 10253
Affinity Capture-MS Homo sapiens
8 ACLY 47
Co-fractionation Homo sapiens
9 GMDS 2762
Co-fractionation Homo sapiens
10 MTPN 136319
Affinity Capture-MS Homo sapiens
11 SDC1 6382
Affinity Capture-MS Homo sapiens
12 LUM 4060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 WDR1 9948
Co-fractionation Homo sapiens
14 TPD52L2 7165
Co-fractionation Homo sapiens
15 HSPE1 3336
Co-fractionation Homo sapiens
16 PFKM 5213
Co-fractionation Homo sapiens
17 RDH12 145226
Affinity Capture-MS Homo sapiens
18 DLD 1738
Co-fractionation Homo sapiens
19 SLC25A32 81034
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PTMA 5757
Co-fractionation Homo sapiens
21 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
23 FSD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
25 YTHDF1 54915
Co-fractionation Homo sapiens
26 FOXI2  
Affinity Capture-MS Homo sapiens
27 PARK2  
Affinity Capture-MS Homo sapiens
28 DUT 1854
Co-fractionation Homo sapiens
29 CTBP1 1487
Co-fractionation Homo sapiens
30 SCG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 GHITM 27069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PTCRA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RPE  
Co-fractionation Homo sapiens
35 MPL 4352
Affinity Capture-MS Homo sapiens
36 ZNF488  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 FOLH1 2346
Co-fractionation Homo sapiens
38 NDUFAF5  
Affinity Capture-MS Homo sapiens
39 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SEC31A 22872
Co-fractionation Homo sapiens
41 WDYHV1  
Two-hybrid Homo sapiens
42 FDPS 2224
Co-fractionation Homo sapiens
43 MRPL46  
Affinity Capture-MS Homo sapiens
44 SERPINB8 5271
Co-fractionation Homo sapiens
45 PAPSS2 9060
Co-fractionation Homo sapiens
46 HOXD3  
Affinity Capture-MS Homo sapiens
47 TP53RK 112858
Co-fractionation Homo sapiens
48 RECK 8434
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 SGTA 6449
Co-fractionation Homo sapiens
50 TP53I3  
Co-fractionation Homo sapiens
51 SDCBP2 27111
Two-hybrid Homo sapiens
52 CTH 1491
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 ZSCAN32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 PLAA 9373
Co-fractionation Homo sapiens
56 SMOC1 64093
Affinity Capture-MS Homo sapiens
57 PREP 5550
Co-fractionation Homo sapiens
58 CUL4A 8451
Affinity Capture-MS Homo sapiens
59 ATIC 471
Co-fractionation Homo sapiens
60 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CAPN2 824
Co-fractionation Homo sapiens
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