Gene description for GHITM
Gene name growth hormone inducible transmembrane protein
Gene symbol GHITM
Other names/aliases DERP2
HSPC282
MICS1
My021
PTD010
TMBIM5
Species Homo sapiens
 Database cross references - GHITM
ExoCarta ExoCarta_27069
Vesiclepedia VP_27069
Entrez Gene 27069
HGNC 17281
UniProt Q9H3K2  
 GHITM identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for GHITM
Molecular Function
    calcium channel activity GO:0005262 IBA
    protein binding GO:0005515 IPI
    calcium:proton antiporter activity GO:0015369 IMP
Biological Process
    calcium ion transport GO:0006816 IMP
    mitochondrial calcium ion transmembrane transport GO:0006851 IMP
    apoptotic process GO:0006915 IEA
    inner mitochondrial membrane organization GO:0007007 IBA
    inner mitochondrial membrane organization GO:0007007 IMP
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IMP
    calcium export from the mitochondrion GO:0099093 IBA
    calcium export from the mitochondrion GO:0099093 IMP
    mitochondrial potassium ion transmembrane transport GO:0140141 IEA
    proton transmembrane transport GO:1902600 IEA
    positive regulation of mitochondrial ATP synthesis coupled electron transport GO:1905448 ISS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    mitochondrial membrane GO:0031966 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GHITM in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for GHITM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 PRPS1L1 221823
Affinity Capture-MS Homo sapiens
4 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MPG 4350
Affinity Capture-MS Homo sapiens
6 ANKLE2 23141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 AGPAT5 55326
Affinity Capture-MS Homo sapiens
8 IZUMO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 JSRP1  
Two-hybrid Homo sapiens
10 CCNB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SYNGR1 9145
Affinity Capture-MS Homo sapiens
12 SLC39A4 55630
Affinity Capture-MS Homo sapiens
13 MTX1 4580
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CCR4 1233
Two-hybrid Homo sapiens
15 ARL15 54622
Affinity Capture-MS Homo sapiens
16 ART1 417
Affinity Capture-MS Homo sapiens
17 DPEP1 1800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NDUFA4 4697
Affinity Capture-MS Homo sapiens
19 PTPN1 5770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
21 FFAR1  
Affinity Capture-MS Homo sapiens
22 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FNDC5  
Affinity Capture-MS Homo sapiens
24 PDE2A  
Affinity Capture-MS Homo sapiens
25 MATN1  
Affinity Capture-MS Homo sapiens
26 GP5 2814
Affinity Capture-MS Homo sapiens
27 PMPCA 23203
Affinity Capture-MS Homo sapiens
28 IGSF8 93185
Affinity Capture-MS Homo sapiens
29 CHCHD2  
Affinity Capture-MS Homo sapiens
30 CHCHD10  
Affinity Capture-MS Homo sapiens
31 MDC1  
Affinity Capture-MS Homo sapiens
32 NT5C3A 51251
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RHOA 387
Affinity Capture-MS Homo sapiens
34 AGTR1  
Two-hybrid Homo sapiens
35 ATP2A3 489
Affinity Capture-MS Homo sapiens
36 PGRMC2 10424
Two-hybrid Homo sapiens
37 C12orf73  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CRACR2B  
Affinity Capture-MS Homo sapiens
39 NAT8B  
Affinity Capture-MS Homo sapiens
40 CYP7B1  
Affinity Capture-MS Homo sapiens
41 NDUFA11 126328
Affinity Capture-MS Homo sapiens
42 HRASLS2  
Affinity Capture-MS Homo sapiens
43 CTH 1491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 UNK  
Affinity Capture-RNA Homo sapiens
45 SLC52A2 79581
Affinity Capture-MS Homo sapiens
46 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 TOR1B 27348
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 COPE 11316
Affinity Capture-MS Homo sapiens
49 MFNG  
Affinity Capture-MS Homo sapiens
50 MTFR1L 56181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 LCTL  
Affinity Capture-MS Homo sapiens
52 ILVBL 10994
Affinity Capture-MS Homo sapiens
53 GPR35  
Two-hybrid Homo sapiens
54 ALPI  
Affinity Capture-MS Homo sapiens
55 FAM162A 26355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 RAP1B 5908
Affinity Capture-MS Homo sapiens
57 SLC5A4  
Affinity Capture-MS Homo sapiens
58 PBXIP1 57326
Affinity Capture-MS Homo sapiens
59 RPA2 6118
Proximity Label-MS Homo sapiens
60 SAR1B 51128
Affinity Capture-MS Homo sapiens
61 GK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 B4GALT3 8703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 DNAJC30  
Proximity Label-MS Homo sapiens
65 TMEM171  
Affinity Capture-MS Homo sapiens
66 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 C11orf31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 NT5E 4907
Affinity Capture-MS Homo sapiens
69 HSPA5 3309
Affinity Capture-MS Homo sapiens
70 VIPR2  
Affinity Capture-MS Homo sapiens
71 RAMP2  
Affinity Capture-MS Homo sapiens
72 LRRN1 57633
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 SLC25A12 8604
Proximity Label-MS Homo sapiens
74 LRRC25  
Affinity Capture-MS Homo sapiens
75 SARM1 23098
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 B3GNT3 10331
Affinity Capture-MS Homo sapiens
77 P2RY2 5029
Affinity Capture-MS Homo sapiens
78 HTR2C  
Two-hybrid Homo sapiens
79 SLTM 79811
Affinity Capture-MS Homo sapiens
80 TMEM242  
Two-hybrid Homo sapiens
81 GCNT2 2651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 CD27  
Affinity Capture-MS Homo sapiens
83 FAM209A 200232
Two-hybrid Homo sapiens
84 CA14 23632
Affinity Capture-MS Homo sapiens
85 CD79A  
Affinity Capture-MS Homo sapiens
86 RPA3 6119
Proximity Label-MS Homo sapiens
87 B3GNT7  
Affinity Capture-MS Homo sapiens
88 EMC2 9694
Affinity Capture-MS Homo sapiens
89 ACKR2  
Affinity Capture-MS Homo sapiens
90 MFN2 9927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 DDRGK1 65992
Affinity Capture-MS Homo sapiens
92 ENTPD2 954
Affinity Capture-MS Homo sapiens
93 NRN1  
Affinity Capture-MS Homo sapiens
94 GPR37  
Two-hybrid Homo sapiens
95 LMNA 4000
Proximity Label-MS Homo sapiens
96 THY1 7070
Affinity Capture-MS Homo sapiens
97 DRD2  
Two-hybrid Homo sapiens
98 UBE2J1 51465
Affinity Capture-MS Homo sapiens
99 CDH13 1012
Affinity Capture-MS Homo sapiens
100 SLC2A12 154091
Affinity Capture-MS Homo sapiens
101 FNDC4 64838
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 FCGR3B  
Affinity Capture-MS Homo sapiens
103 GABRE  
Affinity Capture-MS Homo sapiens
104 TP53 7157
Affinity Capture-MS Homo sapiens
105 SERINC2 347735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 OPALIN  
Affinity Capture-MS Homo sapiens
107 ARF5 381
Affinity Capture-MS Homo sapiens
108 CD1B 910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 CCDC127  
Affinity Capture-MS Homo sapiens
110 ARL8A 127829
Affinity Capture-MS Homo sapiens
111 DNAJA3 9093
Proximity Label-MS Homo sapiens
112 EFNB1 1947
Affinity Capture-MS Homo sapiens
113 SFXN1 94081
Proximity Label-MS Homo sapiens
114 PTPN5  
Affinity Capture-MS Homo sapiens
115 ERGIC2 51290
Affinity Capture-MS Homo sapiens
116 RIOK2 55781
Affinity Capture-MS Homo sapiens
117 HM13 81502
Affinity Capture-MS Homo sapiens
118 BCL2  
Affinity Capture-MS Homo sapiens
119 HTR4  
Two-hybrid Homo sapiens
120 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 POMK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 ORAI1 84876
Affinity Capture-MS Homo sapiens
123 EVA1B 55194
Affinity Capture-MS Homo sapiens
124 VIPR1  
Affinity Capture-MS Homo sapiens
125 PIANP  
Affinity Capture-MS Homo sapiens
126 ND4 4538
Affinity Capture-MS Homo sapiens
127 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GHITM is involved
No pathways found





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