Experiment description of studies that identified THY1 in exosomes

1 Experiment ID 126 ISEV standards ✘ EV Biophysical techniques ✔ GAPDH EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

2 Experiment ID 261 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD81|LAMP2|CD9 EV Membrane markers ✘ EV Negative markers ✔ Y EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 25669974     Organism Homo sapiens Experiment description Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins,and metabolites Authors Krishna C. Vallabhaneni1, Patrice Penfornis, Santosh Dhule, Francois Guillonneau, Kristen V. Adams, Yin Yuan Mo, Rui Xu, Yiming Liu, Kounosuke Watabe, Mohan C. Vemuri and Radhika Pochampally Journal name Oncotarget Publication year 2014 Sample Mesenchymal stem cells Sample name hMSCs Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Lipids Metabolites Methods used in the study Western blotting Mass spectrometry RT-PCR Sequencing

3 Experiment ID 224 ISEV standards ✔ EM|AFM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81 EV Membrane markers ✔ GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

4 Experiment ID 217 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD81|CD9|CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 13 ISEV standards ✔ IEM EV Biophysical techniques ✔ Alix|RAB4|RAB5B|RAB11|TSG101 EV Cytosolic markers ✔ CD9|AQP2|AQP1 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15326289     Organism Homo sapiens Experiment description Identification and proteomic profiling of exosomes in human urine. Authors Pisitkun T, Shen RF, Knepper MA Journal name PNAS Publication year 2004 Sample Urine Sample name Urine - Normal Isolation/purification methods Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

6 Experiment ID 63 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ AQP2 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

7 Experiment ID 193 ISEV standards ✔ EM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✔ PHB EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 194 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of basement membrane nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 195 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✘ EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC. Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of early IgA nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 196 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix|TSG101|HSP70 EV Cytosolic markers ✔ CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G. Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal high density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

11 Experiment ID 197 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix|TSG101|HSP70 EV Cytosolic markers ✔ CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 22418980     Organism Homo sapiens Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G. Journal name Kidney Int Publication year 2012 Sample Urine Sample name Urine - Normal low density Isolation/purification methods Differential centrifugation Sucrose cushion Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for THY1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 FYN 2534 Invivo Homo sapiens Affinity Capture-MS Homo sapiens 2 LCK 3932 Affinity Capture-MS Homo sapiens Invivo Homo sapiens 3 THY1 7070 Invitro Homo sapiens Affinity Capture-MS Homo sapiens Invitro Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which THY1 is involved

No pathways found