Gene description for PIGS
Gene name phosphatidylinositol glycan anchor biosynthesis, class S
Gene symbol PIGS
Other names/aliases -
Species Homo sapiens
 Database cross references - PIGS
ExoCarta ExoCarta_94005
Vesiclepedia VP_94005
Entrez Gene 94005
HGNC 14937
MIM 610271
UniProt Q96S52  
 PIGS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PIGS
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    GPI anchor biosynthetic process GO:0006506 IEA
    attachment of GPI anchor to protein GO:0016255 IBA
    attachment of GPI anchor to protein GO:0016255 NAS
    attachment of GPI anchor to protein GO:0016255 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    GPI-anchor transamidase complex GO:0042765 IBA
    GPI-anchor transamidase complex GO:0042765 IDA
    GPI-anchor transamidase complex GO:0042765 IPI
    GPI-anchor transamidase complex GO:0042765 TAS
 Experiment description of studies that identified PIGS in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PIGS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 HMOX1 3162
Affinity Capture-MS Homo sapiens
3 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
5 DNAJC8 22826
Affinity Capture-MS Homo sapiens
6 VAPA 9218
Affinity Capture-MS Homo sapiens
7 SLC39A4 55630
Affinity Capture-MS Homo sapiens
8 MME 4311
Affinity Capture-MS Homo sapiens
9 CFC1  
Affinity Capture-MS Homo sapiens
10 TMEM95  
Affinity Capture-MS Homo sapiens
11 PLD4  
Affinity Capture-MS Homo sapiens
12 PIGK 10026
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 KIAA1429 25962
Affinity Capture-MS Homo sapiens
14 HYAL1 3373
Affinity Capture-MS Homo sapiens
15 MYH6 4624
Affinity Capture-MS Homo sapiens
16 ACAP2 23527
Affinity Capture-MS Homo sapiens
17 SDHB 6390
Co-fractionation Homo sapiens
18 SF3B1 23451
Affinity Capture-MS Homo sapiens
19 C4orf3  
Affinity Capture-MS Homo sapiens
20 KRT40  
Two-hybrid Homo sapiens
21 GPRC5B 51704
Affinity Capture-MS Homo sapiens
22 SF3A2 8175
Affinity Capture-MS Homo sapiens
23 TTYH1  
Affinity Capture-MS Homo sapiens
24 PPM1A 5494
Affinity Capture-MS Homo sapiens
25 CCDC47 57003
Affinity Capture-MS Homo sapiens
26 Haus1  
Affinity Capture-MS Mus musculus
27 NDUFS1 4719
Affinity Capture-MS Homo sapiens
28 FAM20C 56975
Affinity Capture-MS Homo sapiens
29 LMAN1 3998
Co-fractionation Homo sapiens
30 ENPP6 133121
Affinity Capture-MS Homo sapiens
31 HS2ST1 9653
Affinity Capture-MS Homo sapiens
32 RB1CC1 9821
Affinity Capture-MS Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 CD7  
Affinity Capture-MS Homo sapiens
35 USMG5 84833
Affinity Capture-MS Homo sapiens
36 VAPB 9217
Affinity Capture-MS Homo sapiens
37 SCAMP2 10066
Affinity Capture-MS Homo sapiens
38 PIGT 51604
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
39 RPA4  
Proximity Label-MS Homo sapiens
40 ATP6V1B1 525
Co-fractionation Homo sapiens
41 TMEM131  
Affinity Capture-MS Homo sapiens
42 GPR173  
Affinity Capture-MS Homo sapiens
43 SEC61B 10952
Proximity Label-MS Homo sapiens
44 COX4I1 1327
Affinity Capture-MS Homo sapiens
45 C15orf32  
Affinity Capture-MS Homo sapiens
46 NOTCH2NL 388677
Two-hybrid Homo sapiens
47 HSPA1L 3305
Co-fractionation Homo sapiens
48 VIPR2  
Affinity Capture-MS Homo sapiens
49 GPAA1 8733
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
50 FANCD2  
Affinity Capture-MS Homo sapiens
51 CANX 821
Affinity Capture-MS Homo sapiens
52 LRRC59 55379
Proximity Label-MS Homo sapiens
53 NDUFA9 4704
Affinity Capture-MS Homo sapiens
54 KIRREL2  
Affinity Capture-MS Homo sapiens
55 GPR89B 51463
Affinity Capture-MS Homo sapiens
56 KRTAP10-8  
Two-hybrid Homo sapiens
57 B3GNT3 10331
Affinity Capture-MS Homo sapiens
58 PRSS50  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 EGFR 1956
Co-fractionation Homo sapiens
60 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KRTAP10-3  
Two-hybrid Homo sapiens
62 YIPF5 81555
Affinity Capture-MS Homo sapiens
63 ENTPD7  
Affinity Capture-MS Homo sapiens
64 LPAR1 1902
Affinity Capture-MS Homo sapiens
65 AP1G2 8906
Affinity Capture-MS Homo sapiens
66 EMR4P  
Affinity Capture-MS Homo sapiens
67 TSPAN8 7103
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 CNTROB  
Affinity Capture-MS Homo sapiens
70 FBXO2 26232
Affinity Capture-MS Homo sapiens
71 PSCA 8000
Affinity Capture-MS Homo sapiens
72 ASPH 444
Affinity Capture-MS Homo sapiens
73 ENTPD2 954
Affinity Capture-MS Homo sapiens
74 THY1 7070
Affinity Capture-MS Homo sapiens
75 CLPP 8192
Proximity Label-MS Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 TSPAN5 10098
Affinity Capture-MS Homo sapiens
78 TMED10 10972
Affinity Capture-MS Homo sapiens
79 VNN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 GP9 2815
Affinity Capture-MS Homo sapiens
81 PDIA4 9601
Proximity Label-MS Homo sapiens
82 DNAJA2 10294
Affinity Capture-MS Homo sapiens
83 TSPAN15 23555
Affinity Capture-MS Homo sapiens
84 B4GAT1 11041
Affinity Capture-MS Homo sapiens
85 PIGU 128869
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
86 VAC14 55697
Affinity Capture-MS Homo sapiens
87 ART4  
Affinity Capture-MS Homo sapiens
88 SOAT1 6646
Affinity Capture-MS Homo sapiens
89 SPPL2B 56928
Affinity Capture-MS Homo sapiens
90 RAB7A 7879
Proximity Label-MS Homo sapiens
91 SMPD2 6610
Affinity Capture-MS Homo sapiens
92 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
93 MICALL2 79778
Affinity Capture-MS Homo sapiens
94 CALR3  
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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