Gene description for HLA-DRB1
Gene name major histocompatibility complex, class II, DR beta 1
Gene symbol HLA-DRB1
Other names/aliases DRB1
DRw10
HLA-DR1B
HLA-DRB
SS1
Species Homo sapiens
 Database cross references - HLA-DRB1
ExoCarta ExoCarta_3123
Vesiclepedia VP_3123
Entrez Gene 3123
HGNC 4948
MIM 142857
UniProt P01912   Q29974   P01911  
 HLA-DRB1 identified in exosomes derived from the following tissue/cell type
B cells 12519789    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for HLA-DRB1
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    MHC class II protein complex binding GO:0023026 HDA
    MHC class II protein complex binding GO:0023026 IBA
    polysaccharide binding GO:0030247 IDA
    MHC class II receptor activity GO:0032395 IDA
    MHC class II receptor activity GO:0032395 NAS
    MHC class II receptor activity GO:0032395 TAS
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 IDA
    T cell receptor binding GO:0042608 IDA
    CD4 receptor binding GO:0042609 IPI
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 IDA
    positive regulation of protein phosphorylation GO:0001934 IDA
    inflammatory response to antigenic stimulus GO:0002437 IDA
    myeloid dendritic cell antigen processing and presentation GO:0002469 IDA
    antigen processing and presentation of endogenous peptide antigen via MHC class II GO:0002491 IDA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IBA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IDA
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 IDA
    negative regulation of inflammatory response to antigenic stimulus GO:0002862 IDA
    immune response GO:0006955 IDA
    immune response GO:0006955 IMP
    immune response GO:0006955 NAS
    immune response GO:0006955 TAS
    humoral immune response GO:0006959 IMP
    cytoskeleton organization GO:0007010 IEA
    signal transduction GO:0007165 IMP
    signal transduction GO:0007165 NAS
    epidermis development GO:0008544 TAS
    detection of bacterium GO:0016045 IMP
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    macrophage differentiation GO:0030225 IDA
    regulation of interleukin-10 production GO:0032653 IDA
    regulation of interleukin-4 production GO:0032673 IDA
    negative regulation of type II interferon production GO:0032689 IMP
    positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation GO:0032831 IDA
    positive regulation of kinase activity GO:0033674 IDA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IMP
    T-helper 1 type immune response GO:0042088 IMP
    negative regulation of T cell proliferation GO:0042130 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    positive regulation of memory T cell differentiation GO:0043382 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    regulation of T-helper cell differentiation GO:0045622 IDA
    positive regulation of monocyte differentiation GO:0045657 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of viral entry into host cell GO:0046598 IDA
    positive regulation of immune response GO:0050778 IBA
    T cell receptor signaling pathway GO:0050852 IDA
    positive regulation of T cell activation GO:0050870 IBA
    protein tetramerization GO:0051262 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    positive regulation of CD4-positive, alpha-beta T cell activation GO:2000516 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    immunological synapse GO:0001772 IDA
    extracellular space GO:0005615 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    intermediate filament GO:0005882 TAS
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 NAS
    membrane GO:0016020 TAS
    transport vesicle membrane GO:0030658 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IDA
    trans-Golgi network membrane GO:0032588 TAS
    MHC class II protein complex GO:0042613 IBA
    MHC class II protein complex GO:0042613 IDA
    MHC class II protein complex GO:0042613 IMP
    extracellular exosome GO:0070062 HDA
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
    autolysosome membrane GO:0120281 IEA
 Experiment description of studies that identified HLA-DRB1 in exosomes
1
Experiment ID 11
MISEV standards
Biophysical techniques
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 259
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DRB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ST8SIA3  
Affinity Capture-MS Homo sapiens
3 NDUFB8 4714
Affinity Capture-MS Homo sapiens
4 MAGEA3  
Protein-peptide Homo sapiens
5 SCNN1A  
Affinity Capture-MS Homo sapiens
6 CACNA2D2 9254
Affinity Capture-MS Homo sapiens
7 HLA-E 3133
Affinity Capture-MS Homo sapiens
8 PKM 5315
Affinity Capture-MS Homo sapiens
9 KIAA2013 90231
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
11 CTDNEP1 23399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TUBB8 347688
Affinity Capture-MS Homo sapiens
13 ATP12A 479
Affinity Capture-MS Homo sapiens
14 ECEL1  
Affinity Capture-MS Homo sapiens
15 PHOSPHO1  
Affinity Capture-MS Homo sapiens
16 TRA  
Co-crystal Structure Homo sapiens
17 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
18 TMEM67 91147
Affinity Capture-MS Homo sapiens
19 PDE3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ITGA6 3655
Affinity Capture-MS Homo sapiens
21 PTPRN  
Affinity Capture-MS Homo sapiens
22 ITFG1 81533
Affinity Capture-MS Homo sapiens
23 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SLC39A10 57181
Affinity Capture-MS Homo sapiens
25 LOC100506753  
Protein-RNA Homo sapiens
26 ADAM9 8754
Affinity Capture-MS Homo sapiens
27 NETO2  
Affinity Capture-MS Homo sapiens
28 MST1R 4486
Affinity Capture-MS Homo sapiens
29 TMEM201 199953
Affinity Capture-MS Homo sapiens
30 SQLE 6713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ABCA2 20
Affinity Capture-MS Homo sapiens
32 ATP2A3 489
Affinity Capture-MS Homo sapiens
33 SCARB1 949
Affinity Capture-MS Homo sapiens
34 NMU  
Affinity Capture-MS Homo sapiens
35 DGCR2 9993
Affinity Capture-MS Homo sapiens
36 NDUFV3 4731
Affinity Capture-MS Homo sapiens
37 NUCB1 4924
Affinity Capture-MS Homo sapiens
38 SLC25A31 83447
Affinity Capture-MS Homo sapiens
39 BTNL2  
Affinity Capture-MS Homo sapiens
40 TMEM106A  
Affinity Capture-MS Homo sapiens
41 NDUFA6  
Affinity Capture-MS Homo sapiens
42 MPZL2 10205
Affinity Capture-MS Homo sapiens
43 TMEM59 9528
Affinity Capture-MS Homo sapiens
44 GRIN3B  
Affinity Capture-MS Homo sapiens
45 HLA-G 3135
Affinity Capture-MS Homo sapiens
46 HSPA8 3312
Affinity Capture-MS Homo sapiens
47 TMEM106C 79022
Affinity Capture-MS Homo sapiens
48 PRF1  
Affinity Capture-MS Homo sapiens
49 CADM4 199731
Affinity Capture-MS Homo sapiens
50 ZDHHC17 23390
Two-hybrid Homo sapiens
51 EIF2AK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CNNM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NOMO1 23420
Affinity Capture-MS Homo sapiens
54 ATP1B4  
Affinity Capture-MS Homo sapiens
55 TMED6  
Affinity Capture-MS Homo sapiens
56 NPC1 4864
Affinity Capture-MS Homo sapiens
57 CD74 972
Affinity Capture-MS Homo sapiens
58 CYB5D2  
Affinity Capture-MS Homo sapiens
59 CACNG7  
Affinity Capture-MS Homo sapiens
60 TUBA1A 7846
Affinity Capture-MS Homo sapiens
61 RNF5  
Affinity Capture-MS Homo sapiens
62 VCP 7415
Affinity Capture-Western Homo sapiens
63 TRIM21 6737
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
64 SARAF  
Affinity Capture-MS Homo sapiens
65 HLA-DRB5 3127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MIA3 375056
Affinity Capture-MS Homo sapiens
67 SEMA4F  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PIGS 94005
Affinity Capture-MS Homo sapiens
69 TMEM131  
Affinity Capture-MS Homo sapiens
70 AHCTF1 25909
Affinity Capture-MS Homo sapiens
71 CNDP1  
Affinity Capture-MS Homo sapiens
72 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
73 TMEM106B 54664
Affinity Capture-MS Homo sapiens
74 GPAA1 8733
Affinity Capture-MS Homo sapiens
75 MARCH1  
FRET Homo sapiens
76 NDUFA9 4704
Affinity Capture-MS Homo sapiens
77 CHST8 64377
Affinity Capture-MS Homo sapiens
78 PON2 5445
Affinity Capture-MS Homo sapiens
79 GALNT5 11227
Affinity Capture-MS Homo sapiens
80 RAB29 8934
Affinity Capture-MS Homo sapiens
81 POM121  
Affinity Capture-MS Homo sapiens
82 OGN 4969
Affinity Capture-MS Homo sapiens
83 HLA-F 3134
Affinity Capture-MS Homo sapiens
84 IMPAD1 54928
Affinity Capture-MS Homo sapiens
85 KCNN4 3783
Affinity Capture-MS Homo sapiens
86 ATP1B1 481
Affinity Capture-MS Homo sapiens
87 GXYLT1 283464
Affinity Capture-MS Homo sapiens
88 HLA-DMA  
Reconstituted Complex Homo sapiens
89 ENTPD2 954
Affinity Capture-MS Homo sapiens
90 MPPE1 65258
Affinity Capture-MS Homo sapiens
91 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
92 KIAA0319L 79932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 GFRA3 2676
Affinity Capture-MS Homo sapiens
94 NRG1 3084
Affinity Capture-MS Homo sapiens
95 EMC6 83460
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 FNDC4 64838
Affinity Capture-MS Homo sapiens
97 MR1  
Affinity Capture-MS Homo sapiens
98 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 ASIC4  
Affinity Capture-MS Homo sapiens
100 CD1A 909
Affinity Capture-MS Homo sapiens
101 MS4A1 931
Affinity Capture-MS Homo sapiens
102 EXTL2 2135
Affinity Capture-MS Homo sapiens
103 NDUFA12 55967
Affinity Capture-MS Homo sapiens
104 SSBP2  
Affinity Capture-MS Homo sapiens
105 BTN2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
107 STARD3 10948
Affinity Capture-MS Homo sapiens
108 ROR2 4920
Affinity Capture-MS Homo sapiens
109 MMGT1 93380
Affinity Capture-MS Homo sapiens
110 ANXA11 311
Affinity Capture-MS Homo sapiens
111 MBNL1 4154
Affinity Capture-MS Homo sapiens
112 SIRPD  
Affinity Capture-MS Homo sapiens
113 NDUFA2 4695
Affinity Capture-MS Homo sapiens
114 UGT1A7 54577
Affinity Capture-MS Homo sapiens
115 CLSTN3 9746
Affinity Capture-MS Homo sapiens
116 NAALADL2  
Affinity Capture-MS Homo sapiens
117 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 CLEC4E  
Affinity Capture-MS Homo sapiens
119 HLA-C 3107
Affinity Capture-MS Homo sapiens
120 KIAA0922  
Affinity Capture-MS Homo sapiens
121 NDUFB4 4710
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here