Gene description for CTDNEP1
Gene name CTD nuclear envelope phosphatase 1
Gene symbol CTDNEP1
Other names/aliases DULLARD
HSA011916
NET56
Species Homo sapiens
 Database cross references - CTDNEP1
ExoCarta ExoCarta_23399
Vesiclepedia VP_23399
Entrez Gene 23399
HGNC 19085
MIM 610684
UniProt O95476  
 CTDNEP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for CTDNEP1
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IMP
    protein serine/threonine phosphatase activity GO:0004722 TAS
    protein binding GO:0005515 IPI
    myosin phosphatase activity GO:0017018 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 IDA
    nuclear envelope organization GO:0006998 IDA
    mitotic nuclear membrane disassembly GO:0007077 TAS
    gamete generation GO:0007276 IEA
    mesoderm development GO:0007498 IEA
    positive regulation of triglyceride biosynthetic process GO:0010867 IDA
    protein localization to nucleus GO:0034504 IDA
    canonical Wnt signaling pathway GO:0060070 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    lipid droplet GO:0005811 IDA
    nuclear membrane GO:0031965 IDA
    Nem1-Spo7 phosphatase complex GO:0071595 IDA
 Experiment description of studies that identified CTDNEP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for CTDNEP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 FAM189B  
Affinity Capture-MS Homo sapiens
3 SLC17A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KCNMB3  
Affinity Capture-MS Homo sapiens
5 POR 5447
Proximity Label-MS Homo sapiens
6 ALDH3A2 224
Proximity Label-MS Homo sapiens
7 TRIM13  
Proximity Label-MS Homo sapiens
8 ITPR2 3709
Proximity Label-MS Homo sapiens
9 EMC3 55831
Proximity Label-MS Homo sapiens
10 TGFBR2 7048
Affinity Capture-MS Homo sapiens
11 OR10H2  
Affinity Capture-MS Homo sapiens
12 EMC1 23065
Proximity Label-MS Homo sapiens
13 CDCA3 83461
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 SRPRB 58477
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PDZD8 118987
Proximity Label-MS Homo sapiens
16 IL20RB  
Affinity Capture-MS Homo sapiens
17 UBQLN2 29978
Two-hybrid Homo sapiens
18 CNEP1R1 255919
Affinity Capture-Western Homo sapiens
19 VEZT 55591
Proximity Label-MS Homo sapiens
20 IL22RA1 58985
Affinity Capture-MS Homo sapiens
21 SENP2 59343
Proximity Label-MS Homo sapiens
22 VPS13A 23230
Proximity Label-MS Homo sapiens
23 SMPD4 55627
Proximity Label-MS Homo sapiens
24 TLR5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 EGFR 1956
Negative Genetic Homo sapiens
26 SYNE2 23224
Proximity Label-MS Homo sapiens
27 ITPR3 3710
Proximity Label-MS Homo sapiens
28 AAAS 8086
Proximity Label-MS Homo sapiens
29 PDGFRB 5159
Affinity Capture-MS Homo sapiens
30 ARL6IP1 23204
Affinity Capture-MS Homo sapiens
31 SAAL1 113174
Affinity Capture-MS Homo sapiens
32 DDX58 23586
Affinity Capture-RNA Homo sapiens
33 GNAI1 2770
Affinity Capture-MS Homo sapiens
34 TUBB6 84617
Affinity Capture-MS Homo sapiens
35 OCIAD1 54940
Proximity Label-MS Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 GRAMD1A  
Proximity Label-MS Homo sapiens
38 APEX1 328
Affinity Capture-RNA Homo sapiens
39 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MPL 4352
Affinity Capture-MS Homo sapiens
41 KCNE3  
Affinity Capture-MS Homo sapiens
42 TEX264 51368
Affinity Capture-MS Homo sapiens
43 SUN1 23353
Proximity Label-MS Homo sapiens
44 MOV10 4343
Affinity Capture-RNA Homo sapiens
45 ATP6AP1 537
Proximity Label-MS Homo sapiens
46 NDC1 55706
Proximity Label-MS Homo sapiens
47 PTPN1 5770
Proximity Label-MS Homo sapiens
48 SUN2 25777
Proximity Label-MS Homo sapiens
49 HAX1  
Affinity Capture-MS Homo sapiens
50 CCDC107  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 OLFM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 FAM189A2  
Affinity Capture-MS Homo sapiens
53 ITPR1 3708
Proximity Label-MS Homo sapiens
54 PLXDC2 84898
Affinity Capture-MS Homo sapiens
55 CUL7 9820
Affinity Capture-MS Homo sapiens
56 VRK2 7444
Proximity Label-MS Homo sapiens
57 ABCD3 5825
Proximity Label-MS Homo sapiens
58 KCNK18  
Affinity Capture-MS Homo sapiens
59 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ATP2A2 488
Affinity Capture-MS Homo sapiens
61 LEMD3  
Proximity Label-MS Homo sapiens
62 CCDC47 57003
Proximity Label-MS Homo sapiens
63 PGRMC2 10424
Proximity Label-MS Homo sapiens
64 C3orf52  
Affinity Capture-MS Homo sapiens
65 TMEM92  
Affinity Capture-MS Homo sapiens
66 ACBD5 91452
Proximity Label-MS Homo sapiens
67 EDAR  
Affinity Capture-MS Homo sapiens
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