Experiment description of studies that identified CDCA3 in exosomes

1 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

Protein-protein interactions for CDCA3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 GJD3   Proximity Label-MS Homo sapiens 2 SKP1 6500 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Co-fractionation Homo sapiens 3 Sgol2   Affinity Capture-MS Mus musculus 4 Cul1 26965 Affinity Capture-MS Mus musculus 5 REEP5 7905 Proximity Label-MS Homo sapiens 6 OCLN 100506658 Proximity Label-MS Homo sapiens 7 METTL7A 25840 Proximity Label-MS Homo sapiens 8 LAMP3   Proximity Label-MS Homo sapiens 9 ATG9A 79065 Proximity Label-MS Homo sapiens 10 GJA1 2697 Proximity Label-MS Homo sapiens 11 ATP6V1C1 528 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 Nek2   Affinity Capture-MS Mus musculus 13 CTDNEP1 23399 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 LAMP2 3920 Proximity Label-MS Homo sapiens 15 STX4 6810 Proximity Label-MS Homo sapiens 16 PLOD1 5351 Co-fractionation Homo sapiens 17 APP 351 Reconstituted Complex Homo sapiens 18 APEX1 328 Affinity Capture-RNA Homo sapiens 19 FZR1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 CTDSP2 10106 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 21 FIS1 51024 Proximity Label-MS Homo sapiens 22 MLLT4 4301 Proximity Label-MS Homo sapiens 23 RAB3B 5865 Proximity Label-MS Homo sapiens 24 Cep170   Affinity Capture-MS Mus musculus 25 STX7 8417 Proximity Label-MS Homo sapiens 26 ATP6V0D2 245972 Affinity Capture-MS Homo sapiens 27 CHORDC1 26973 Affinity Capture-MS Homo sapiens 28 CDC27 996 Affinity Capture-MS Homo sapiens 29 CHMP4A 29082 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 LAMTOR4 389541 Affinity Capture-MS Homo sapiens 31 MAVS 57506 Proximity Label-MS Homo sapiens 32 RAB35 11021 Proximity Label-MS Homo sapiens 33 NMT1 4836 Affinity Capture-MS Homo sapiens 34 EBAG9 9166 Proximity Label-MS Homo sapiens 35 MARCKS 4082 Proximity Label-MS Homo sapiens 36 LAMTOR1 55004 Proximity Label-MS Homo sapiens 37 RAB5A 5868 Proximity Label-MS Homo sapiens 38 MTCH2 23788 Proximity Label-MS Homo sapiens 39 CYP2S1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 EMD 2010 Proximity Label-MS Homo sapiens 41 CXADR 1525 Proximity Label-MS Homo sapiens 42 LYN 4067 Proximity Label-MS Homo sapiens 43 MCAM 4162 Proximity Label-MS Homo sapiens 44 RAB4A 5867 Proximity Label-MS Homo sapiens 45 CERS2 29956 Affinity Capture-MS Homo sapiens 46 DNAJC5 80331 Proximity Label-MS Homo sapiens 47 CDH1 999 Proximity Label-MS Homo sapiens 48 DIRAS3   Proximity Label-MS Homo sapiens 49 Chmp4c   Affinity Capture-MS Mus musculus 50 CDK5R1   Affinity Capture-MS Homo sapiens 51 STX6 10228 Proximity Label-MS Homo sapiens 52 Cdca8   Affinity Capture-MS Mus musculus 53 PARD6B 84612 Affinity Capture-MS Homo sapiens 54 RHOT2 89941 Proximity Label-MS Homo sapiens 55 ELOVL5 60481 Proximity Label-MS Homo sapiens 56 CTDSPL 10217 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 57 SEC61B 10952 Proximity Label-MS Homo sapiens 58 MYH4   Affinity Capture-MS Homo sapiens 59 RMDN3 55177 Proximity Label-MS Homo sapiens 60 COX4I1 1327 Proximity Label-MS Homo sapiens 61 BAG3 9531 Affinity Capture-Luminescence Homo sapiens 62 BCL11A 53335 Two-hybrid Homo sapiens 63 SLC25A12 8604 Proximity Label-MS Homo sapiens 64 RPN2 6185 Proximity Label-MS Homo sapiens 65 AIFM1 9131 Proximity Label-MS Homo sapiens 66 RAB7A 7879 Proximity Label-MS Homo sapiens 67 SDC1 6382 Affinity Capture-MS Homo sapiens 68 LRRC59 55379 Proximity Label-MS Homo sapiens 69 PANX1 24145 Proximity Label-MS Homo sapiens 70 LAMP1 3916 Proximity Label-MS Homo sapiens 71 CIR1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 72 HSD3B7 80270 Proximity Label-MS Homo sapiens 73 STIM1 6786 Proximity Label-MS Homo sapiens 74 PCDHGC3 5098 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 75 FKBP8 23770 Proximity Label-MS Homo sapiens 76 ARF6 382 Proximity Label-MS Homo sapiens 77 CUL1 8454 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 78 SLC25A41   Affinity Capture-MS Homo sapiens 79 WEE1 7465 Reconstituted Complex Homo sapiens 80 OCIAD1 54940 Proximity Label-MS Homo sapiens 81 PRKACA 5566 Proximity Label-MS Homo sapiens 82 RAB9A 9367 Proximity Label-MS Homo sapiens 83 B3GAT1   Proximity Label-MS Homo sapiens 84 Bub1b 12236 Affinity Capture-MS Mus musculus 85 RHOB 388 Proximity Label-MS Homo sapiens 86 AKAP1 8165 Proximity Label-MS Homo sapiens 87 FLOT1 10211 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 88 RNF43   Proximity Label-MS Homo sapiens 89 CS 1431 Proximity Label-MS Homo sapiens 90 TRAF2 7186 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 91 CTDSP1 58190 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 92 EZR 7430 Proximity Label-MS Homo sapiens 93 LCK 3932 Proximity Label-MS Homo sapiens 94 FLOT2 2319 Affinity Capture-MS Homo sapiens 95 Shoc2   Affinity Capture-MS Mus musculus 96 GTF2H5   Affinity Capture-MS Homo sapiens 97 C11orf52 91894 Proximity Label-MS Homo sapiens 98 ARF4 378 Proximity Label-MS Homo sapiens 99 SFXN1 94081 Proximity Label-MS Homo sapiens 100 RAB2A 5862 Proximity Label-MS Homo sapiens 101 SEC62 7095 Proximity Label-MS Homo sapiens 102 DNAJC5B   Proximity Label-MS Homo sapiens 103 GSX1   Affinity Capture-MS Homo sapiens 104 C19orf52   Proximity Label-MS Homo sapiens 105 SLC25A51   Proximity Label-MS Homo sapiens 106 RAB11A 8766 Proximity Label-MS Homo sapiens 107 Cnot2   Affinity Capture-MS Mus musculus 108 KRAS 3845 Proximity Label-MS Homo sapiens 109 RAB5C 5878 Proximity Label-MS Homo sapiens 110 CKAP4 10970 Proximity Label-MS Homo sapiens 111 CCR1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 112 CAV1 857 Proximity Label-MS Homo sapiens 113 TMEM185A   Affinity Capture-MS Homo sapiens 114 DERL1 79139 Proximity Label-MS Homo sapiens 115 TRAF1 7185 Two-hybrid Homo sapiens View the network

Pathways in which CDCA3 is involved

No pathways found