Gene description for PRKACA
Gene name protein kinase, cAMP-dependent, catalytic, alpha
Gene symbol PRKACA
Other names/aliases PKACA
PPNAD4
Species Homo sapiens
 Database cross references - PRKACA
ExoCarta ExoCarta_5566
Entrez Gene 5566
HGNC 9380
MIM 601639
UniProt P17612  
 PRKACA identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for PRKACA
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IDA
    cAMP-dependent protein kinase activity GO:0004691 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase A regulatory subunit binding GO:0034237 IPI
    protein kinase binding GO:0019901 IPI
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
Biological Process
    peptidyl-serine phosphorylation GO:0018105 IDA
    calcium-mediated signaling using intracellular calcium source GO:0035584 TAS
    regulation of bicellular tight junction assembly GO:2000810 IDA
    cellular response to epinephrine stimulus GO:0071872 TAS
    intracellular signal transduction GO:0035556 TAS
    regulation of insulin secretion GO:0050796 TAS
    regulation of proteasomal protein catabolic process GO:0061136 IDA
    innate immune response GO:0045087 TAS
    mesoderm formation GO:0001707 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    regulation of osteoblast differentiation GO:0045667 IDA
    transmembrane transport GO:0055085 TAS
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 TAS
    cellular response to glucagon stimulus GO:0071377 TAS
    peptidyl-threonine phosphorylation GO:0018107 IEA
    small molecule metabolic process GO:0044281 TAS
    sperm capacitation GO:0048240 ISS
    mitotic cell cycle GO:0000278 TAS
    negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning GO:1901621 IEA
    signal transduction GO:0007165 TAS
    regulation of cardiac muscle contraction GO:0055117 TAS
    renal water homeostasis GO:0003091 TAS
    protein phosphorylation GO:0006468 NAS
    protein autophosphorylation GO:0046777 IEA
    cellular response to parathyroid hormone stimulus GO:0071374 IEA
    regulation of protein binding GO:0043393 TAS
    regulation of protein processing GO:0070613 IEA
    energy reserve metabolic process GO:0006112 TAS
    neural tube closure GO:0001843 IEA
    regulation of heart rate GO:0002027 TAS
    activation of protein kinase A activity GO:0034199 TAS
    positive regulation of protein export from nucleus GO:0046827 IEA
    carbohydrate metabolic process GO:0005975 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    regulation of ryanodine-sensitive calcium-release channel activity GO:0060314 TAS
    cytosolic calcium ion homeostasis GO:0051480 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    regulation of synaptic transmission GO:0050804 IEA
    blood coagulation GO:0007596 TAS
    activation of phospholipase C activity GO:0007202 TAS
    cellular response to glucose stimulus GO:0071333 IDA
    organelle organization GO:0006996 TAS
    water transport GO:0006833 TAS
    positive regulation of cell cycle arrest GO:0071158 ISS
    triglyceride catabolic process GO:0019433 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    gluconeogenesis GO:0006094 TAS
    glucose metabolic process GO:0006006 TAS
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    mitochondrion GO:0005739 IEA
    AMP-activated protein kinase complex GO:0031588 IDA
    cAMP-dependent protein kinase complex GO:0005952 NAS
    nucleus GO:0005634 IDA
    neuromuscular junction GO:0031594 IEA
    calcium channel complex GO:0034704 TAS
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
    centrosome GO:0005813 IDA
    plasma membrane GO:0005886 IEA
    motile cilium GO:0031514 IEA
    membrane GO:0016020 IDA
    sperm midpiece GO:0097225 IDA
    ciliary base GO:0097546 TAS
 Experiment description of studies that identified PRKACA in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRKACA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAD  
Reconstituted Complex Homo sapiens
2 ACCN3  
Biochemical Activity Homo sapiens
3 GAD2  
Biochemical Activity Homo sapiens
4 PKIG  
Affinity Capture-MS Homo sapiens
5 RASGRP3  
Biochemical Activity Homo sapiens
6 GABRB3 2562
Reconstituted Complex Homo sapiens
7 ACCN2  
Biochemical Activity Homo sapiens
8 RYR2 6262
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
9 C11orf17  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 GAD1  
Biochemical Activity Homo sapiens
11 EGFR 1956
Affinity Capture-Western Homo sapiens
12 IRF2 3660
Biochemical Activity Homo sapiens
13 PDPK1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
14 CREB1  
Reconstituted Complex Homo sapiens
15 CCC2  
Biochemical Activity 4932
16 KCNQ1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 NIN 51199
Biochemical Activity Homo sapiens
18 PPP1R8 5511
Biochemical Activity Homo sapiens
19 PKIA  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
20 HAND1  
Biochemical Activity Homo sapiens
21 HAND2  
Biochemical Activity Homo sapiens
22 ITPKB  
Invitro Homo sapiens
23 TRIP10 9322
Biochemical Activity Homo sapiens
24 IQGAP1 8826
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
25 CAV1 857
Affinity Capture-Western Homo sapiens
26 CAMKK2 10645
Biochemical Activity Homo sapiens
27 PRKAR1A 5573
Affinity Capture-MS Homo sapiens
28 MGMT 4255
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which PRKACA is involved
PathwayEvidenceSource
Anchoring of the basal body to the plasma membrane TAS Reactome
CD209 (DC-SIGN) signaling TAS Reactome
CREB phosphorylation through the activation of Adenylate Cyclase TAS Reactome
DARPP-32 events TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Degradation of GLI2 by the proteasome TAS Reactome
Factors involved in megakaryocyte development and platelet production TAS Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion TAS Reactome
Gluconeogenesis TAS Reactome
Hedgehog 'off' state TAS Reactome
Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis TAS Reactome
Interleukin-3, 5 and GM-CSF signaling TAS Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
PKA activation TAS Reactome
PKA activation TAS Reactome
PKA activation in glucagon signalling TAS Reactome
PKA-mediated phosphorylation of CREB TAS Reactome
PKA-mediated phosphorylation of CREB TAS Reactome
PKA-mediated phosphorylation of key metabolic factors TAS Reactome
Rap1 signalling TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Regulation of insulin secretion TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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