Gene description for UBXN4
Gene name UBX domain protein 4
Gene symbol UBXN4
Other names/aliases UBXD2
UBXDC1
erasin
Species Homo sapiens
 Database cross references - UBXN4
ExoCarta ExoCarta_23190
Vesiclepedia VP_23190
Entrez Gene 23190
HGNC 14860
MIM 611216
UniProt Q92575  
 UBXN4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UBXN4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    response to unfolded protein GO:0006986 IEA
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IMP
Subcellular Localization
    nuclear envelope GO:0005635 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified UBXN4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UBXN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-Western Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 ATPIF1 93974
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
4 MTCH1 23787
Proximity Label-MS Homo sapiens
5 DERL2 51009
Affinity Capture-MS Homo sapiens
6 C1orf27 54953
Affinity Capture-MS Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 LAMP3  
Proximity Label-MS Homo sapiens
9 ATG9A 79065
Proximity Label-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 VPS13A 23230
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 C9orf78 51759
Affinity Capture-MS Homo sapiens
13 GJD3  
Proximity Label-MS Homo sapiens
14 LAMP2 3920
Proximity Label-MS Homo sapiens
15 DNAJC25 548645
Proximity Label-MS Homo sapiens
16 VPS13C 54832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PTPN1 5770
Proximity Label-MS Homo sapiens
18 GNGT1  
Two-hybrid Homo sapiens
19 STX4 6810
Proximity Label-MS Homo sapiens
20 FIS1 51024
Proximity Label-MS Homo sapiens
21 LOC100506753  
Protein-RNA Homo sapiens
22 AMFR 267
Affinity Capture-Western Homo sapiens
23 RAB3B 5865
Proximity Label-MS Homo sapiens
24 SEL1L 6400
Affinity Capture-Western Homo sapiens
25 PPP1R7 5510
Affinity Capture-Western Homo sapiens
26 SSR1 6745
Proximity Label-MS Homo sapiens
27 MARCH5  
Proximity Label-MS Homo sapiens
28 UBQLN1 29979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
29 FBXO11  
Affinity Capture-MS Homo sapiens
30 LAMTOR3 8649
Cross-Linking-MS (XL-MS) Homo sapiens
31 C3orf52  
Affinity Capture-MS Homo sapiens
32 EXD2  
Proximity Label-MS Homo sapiens
33 DNAJC16  
Proximity Label-MS Homo sapiens
34 SLC25A46 91137
Proximity Label-MS Homo sapiens
35 NSFL1C 55968
Co-fractionation Homo sapiens
36 RAB35 11021
Proximity Label-MS Homo sapiens
37 MAD2L1 4085
Affinity Capture-MS Homo sapiens
38 FAM20C 56975
Affinity Capture-MS Homo sapiens
39 MARCKS 4082
Proximity Label-MS Homo sapiens
40 LMAN1 3998
Proximity Label-MS Homo sapiens
41 NUP155 9631
Proximity Label-MS Homo sapiens
42 KIAA1715 80856
Proximity Label-MS Homo sapiens
43 PXMP2  
Proximity Label-MS Homo sapiens
44 RAB5A 5868
Proximity Label-MS Homo sapiens
45 ACTA2 59
Affinity Capture-MS Homo sapiens
46 PANX1 24145
Proximity Label-MS Homo sapiens
47 MTCH2 23788
Proximity Label-MS Homo sapiens
48 EMD 2010
Proximity Label-MS Homo sapiens
49 PGAM5 192111
Co-fractionation Homo sapiens
50 CXADR 1525
Proximity Label-MS Homo sapiens
51 RAB4A 5867
Proximity Label-MS Homo sapiens
52 CAST 831
Cross-Linking-MS (XL-MS) Homo sapiens
53 DIRAS3  
Proximity Label-MS Homo sapiens
54 VCP 7415
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
55 SLC27A4 10999
Affinity Capture-MS Homo sapiens
56 OPA1 4976
Proximity Label-MS Homo sapiens
57 PEX14 5195
Proximity Label-MS Homo sapiens
58 STX6 10228
Proximity Label-MS Homo sapiens
59 RPN1 6184
Proximity Label-MS Homo sapiens
60 LAGE3  
Affinity Capture-MS Homo sapiens
61 RHOT2 89941
Proximity Label-MS Homo sapiens
62 ELOVL5 60481
Proximity Label-MS Homo sapiens
63 MAVS 57506
Proximity Label-MS Homo sapiens
64 UBXN6 80700
Affinity Capture-MS Homo sapiens
65 RMDN3 55177
Proximity Label-MS Homo sapiens
66 REEP5 7905
Proximity Label-MS Homo sapiens
67 ANLN 54443
Affinity Capture-MS Homo sapiens
68 BCL11A 53335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SEC63 11231
Proximity Label-MS Homo sapiens
70 ASCC3 10973
Cross-Linking-MS (XL-MS) Homo sapiens
71 RPN2 6185
Proximity Label-MS Homo sapiens
72 AIFM1 9131
Proximity Label-MS Homo sapiens
73 CANX 821
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
74 LRRC59 55379
Proximity Label-MS Homo sapiens
75 LMNB1 4001
Proximity Label-MS Homo sapiens
76 MTX2 10651
Proximity Label-MS Homo sapiens
77 TMEM134  
Affinity Capture-MS Homo sapiens
78 LAMP1 3916
Proximity Label-MS Homo sapiens
79 BCAP31 10134
Proximity Label-MS Homo sapiens
80 HSD3B7 80270
Proximity Label-MS Homo sapiens
81 STIM1 6786
Proximity Label-MS Homo sapiens
82 FKBP8 23770
Proximity Label-MS Homo sapiens
83 ARF6 382
Proximity Label-MS Homo sapiens
84 SNX27 81609
Affinity Capture-MS Homo sapiens
85 OCIAD1 54940
Proximity Label-MS Homo sapiens
86 AMY1C 278
Affinity Capture-MS Homo sapiens
87 PRKACA 5566
Proximity Label-MS Homo sapiens
88 FGFR2 2263
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 RPA3 6119
Proximity Label-MS Homo sapiens
90 ATP2A1 487
Proximity Label-MS Homo sapiens
91 RAB9A 9367
Proximity Label-MS Homo sapiens
92 DHFRL1  
Proximity Label-MS Homo sapiens
93 MFN2 9927
Proximity Label-MS Homo sapiens
94 BANF1 8815
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
95 CYP2C9  
Proximity Label-MS Homo sapiens
96 PEX3 8504
Proximity Label-MS Homo sapiens
97 AKAP1 8165
Proximity Label-MS Homo sapiens
98 ACBD5 91452
Proximity Label-MS Homo sapiens
99 RNF43  
Proximity Label-MS Homo sapiens
100 TOM1L1 10040
Two-hybrid Homo sapiens
101 TPTE  
Proximity Label-MS Homo sapiens
102 EDEM1  
Affinity Capture-MS Homo sapiens
103 UBE4A 9354
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
105 ERGIC1 57222
Proximity Label-MS Homo sapiens
106 IP6K1 9807
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 SFXN1 94081
Proximity Label-MS Homo sapiens
108 ERGIC2 51290
Proximity Label-MS Homo sapiens
109 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 SEC62 7095
Proximity Label-MS Homo sapiens
111 B3GAT1  
Proximity Label-MS Homo sapiens
112 RAB7A 7879
Proximity Label-MS Homo sapiens
113 ZG16B 124220
Affinity Capture-MS Homo sapiens
114 RAB11A 8766
Proximity Label-MS Homo sapiens
115 RPS7 6201
Cross-Linking-MS (XL-MS) Homo sapiens
116 STT3B 201595
Affinity Capture-MS Homo sapiens
117 DNAJC1 64215
Proximity Label-MS Homo sapiens
118 KRAS 3845
Proximity Label-MS Homo sapiens
119 RAB5C 5878
Proximity Label-MS Homo sapiens
120 CCDC47 57003
Affinity Capture-MS Homo sapiens
121 CKAP4 10970
Proximity Label-MS Homo sapiens
122 DPP8 54878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 DERL1 79139
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
124 DNAJC22  
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which UBXN4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here