Gene description for DNAJC1
Gene name DnaJ (Hsp40) homolog, subfamily C, member 1
Gene symbol DNAJC1
Other names/aliases DNAJL1
ERdj1
HTJ1
MTJ1
Species Homo sapiens
 Database cross references - DNAJC1
ExoCarta ExoCarta_64215
Vesiclepedia VP_64215
Entrez Gene 64215
HGNC 20090
MIM 611207
UniProt Q96KC8  
 DNAJC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DNAJC1
Molecular Function
    ATPase activator activity GO:0001671 TAS
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    protein-folding chaperone binding GO:0051087 IEA
Biological Process
    regulation of translation GO:0006417 IEA
    protein folding GO:0006457 IEA
    negative regulation of proteolysis GO:0045861 TAS
    regulation of protein secretion GO:0050708 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 IEA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 HDA
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified DNAJC1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DNAJC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Proximity Label-MS Homo sapiens
2 ITGB1 3688
Proximity Label-MS Homo sapiens
3 SCFD1 23256
Proximity Label-MS Homo sapiens
4 MRPS27 23107
Affinity Capture-MS Homo sapiens
5 VPS35 55737
Affinity Capture-MS Homo sapiens
6 SLC4A7 9497
Proximity Label-MS Homo sapiens
7 HSD17B11 51170
Proximity Label-MS Homo sapiens
8 SENP2 59343
Proximity Label-MS Homo sapiens
9 ARHGAP1 392
Proximity Label-MS Homo sapiens
10 EMC2 9694
Proximity Label-MS Homo sapiens
11 ANKLE2 23141
Proximity Label-MS Homo sapiens
12 TYW1  
Proximity Label-MS Homo sapiens
13 KIF16B 55614
Proximity Label-MS Homo sapiens
14 CCPG1 9236
Proximity Label-MS Homo sapiens
15 VAPA 9218
Proximity Label-MS Homo sapiens
16 METTL7A 25840
Proximity Label-MS Homo sapiens
17 DHRS7 51635
Proximity Label-MS Homo sapiens
18 RPL30 6156
Affinity Capture-MS Homo sapiens
19 IPO7 10527
Proximity Label-MS Homo sapiens
20 STIM2 57620
Proximity Label-MS Homo sapiens
21 SUCO  
Proximity Label-MS Homo sapiens
22 ARL6IP5 10550
Proximity Label-MS Homo sapiens
23 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 AKAP11 11215
Proximity Label-MS Homo sapiens
25 MED29  
Affinity Capture-MS Homo sapiens
26 RPL37A 6168
Affinity Capture-MS Homo sapiens
27 CAND2 23066
Affinity Capture-MS Homo sapiens
28 RPL9 6133
Affinity Capture-MS Homo sapiens
29 INF2 64423
Proximity Label-MS Homo sapiens
30 C15orf41  
Affinity Capture-MS Homo sapiens
31 GJD3  
Proximity Label-MS Homo sapiens
32 LAMP2 3920
Proximity Label-MS Homo sapiens
33 DNAJC25 548645
Proximity Label-MS Homo sapiens
34 TBC1D8  
Proximity Label-MS Homo sapiens
35 LNPEP 4012
Proximity Label-MS Homo sapiens
36 PTPN1 5770
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
37 APBB1  
Proximity Label-MS Homo sapiens
38 STX4 6810
Proximity Label-MS Homo sapiens
39 VPS13A 23230
Proximity Label-MS Homo sapiens
40 RTN4 57142
Proximity Label-MS Homo sapiens
41 SLC12A2 6558
Proximity Label-MS Homo sapiens
42 ITPR3 3710
Proximity Label-MS Homo sapiens
43 LGALS3BP 3959
Proximity Label-MS Homo sapiens
44 BNIP1 662
Proximity Label-MS Homo sapiens
45 VANGL2  
Proximity Label-MS Homo sapiens
46 RPL4 6124
Affinity Capture-MS Homo sapiens
47 RPL10A 4736
Affinity Capture-MS Homo sapiens
48 TEX264 51368
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
49 B3GAT1  
Proximity Label-MS Homo sapiens
50 HEATR3 55027
Affinity Capture-MS Homo sapiens
51 WDR41  
Proximity Label-MS Homo sapiens
52 ZC3H15 55854
Proximity Label-MS Homo sapiens
53 RINT1 60561
Proximity Label-MS Homo sapiens
54 FNDC3A 22862
Proximity Label-MS Homo sapiens
55 GOLGA3 2802
Proximity Label-MS Homo sapiens
56 SENP1  
Proximity Label-MS Homo sapiens
57 SMPD4 55627
Proximity Label-MS Homo sapiens
58 EMC4 51234
Proximity Label-MS Homo sapiens
59 RCN2 5955
Affinity Capture-MS Homo sapiens
60 RAB3B 5865
Proximity Label-MS Homo sapiens
61 DRG1 4733
Proximity Label-MS Homo sapiens
62 CPD 1362
Proximity Label-MS Homo sapiens
63 ATF6B  
Proximity Label-MS Homo sapiens
64 ITPR2 3709
Proximity Label-MS Homo sapiens
65 SRRM2 23524
Affinity Capture-MS Homo sapiens
66 CISD2 493856
Proximity Label-MS Homo sapiens
67 PPP6R1 22870
Affinity Capture-MS Homo sapiens
68 PNPLA6 10908
Proximity Label-MS Homo sapiens
69 SSR1 6745
Proximity Label-MS Homo sapiens
70 TMEM201 199953
Two-hybrid Homo sapiens
71 HADHA 3030
Affinity Capture-MS Homo sapiens
72 RWDD1 51389
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
73 PGRMC2 10424
Proximity Label-MS Homo sapiens
74 TTYH1  
Affinity Capture-MS Homo sapiens
75 USP33  
Proximity Label-MS Homo sapiens
76 SYNE3 161176
Proximity Label-MS Homo sapiens
77 CCDC47 57003
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
78 CCDC115 84317
Proximity Label-MS Homo sapiens
79 MXRA7 439921
Proximity Label-MS Homo sapiens
80 LSG1  
Proximity Label-MS Homo sapiens
81 MTDH 92140
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
82 SRPR 6734
Proximity Label-MS Homo sapiens
83 PPP1R15B  
Proximity Label-MS Homo sapiens
84 NUP133 55746
Proximity Label-MS Homo sapiens
85 ESYT2 57488
Proximity Label-MS Homo sapiens
86 FAF2 23197
Proximity Label-MS Homo sapiens
87 SYNM 23336
Proximity Label-MS Homo sapiens
88 DDX54 79039
Proximity Label-MS Homo sapiens
89 ATP6AP1 537
Proximity Label-MS Homo sapiens
90 MARCKS 4082
Proximity Label-MS Homo sapiens
91 IFT20 90410
Affinity Capture-MS Homo sapiens
92 HSPA8 3312
Co-fractionation Homo sapiens
93 FER 2241
Co-fractionation Homo sapiens
94 CAMLG 819
Proximity Label-MS Homo sapiens
95 ECD  
Proximity Label-MS Homo sapiens
96 NUP155 9631
Proximity Label-MS Homo sapiens
97 KIAA1715 80856
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
98 PXMP2  
Proximity Label-MS Homo sapiens
99 RPL27 6155
Affinity Capture-MS Homo sapiens
100 EIF2AK3  
Proximity Label-MS Homo sapiens
101 PANX1 24145
Proximity Label-MS Homo sapiens
102 CHMP7 91782
Proximity Label-MS Homo sapiens
103 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 VAV1 7409
Affinity Capture-MS Homo sapiens
105 CMAS 55907
Affinity Capture-MS Homo sapiens
106 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
107 DDX21 9188
Affinity Capture-MS Homo sapiens
108 STBD1 8987
Proximity Label-MS Homo sapiens
109 EMD 2010
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
110 ITPR1 3708
Proximity Label-MS Homo sapiens
111 KIDINS220 57498
Proximity Label-MS Homo sapiens
112 SRPRB 58477
Proximity Label-MS Homo sapiens
113 REEP4  
Proximity Label-MS Homo sapiens
114 TBL2 26608
Affinity Capture-MS Homo sapiens
115 STOM 2040
Proximity Label-MS Homo sapiens
116 MSX2  
Affinity Capture-MS Homo sapiens
117 CYP4F2  
Two-hybrid Homo sapiens
118 SNAP47 116841
Proximity Label-MS Homo sapiens
119 AGTRAP 57085
Two-hybrid Homo sapiens
120 CDK5RAP3 80279
Proximity Label-MS Homo sapiens
121 RPL5 6125
Affinity Capture-MS Homo sapiens
122 DSG2 1829
Proximity Label-MS Homo sapiens
123 CDKAL1  
Proximity Label-MS Homo sapiens
124 GRAMD1B  
Proximity Label-MS Homo sapiens
125 CLCC1 23155
Proximity Label-MS Homo sapiens
126 FGG 2266
Affinity Capture-MS Homo sapiens
127 AUP1 550
Proximity Label-MS Homo sapiens
128 VAPB 9217
Proximity Label-MS Homo sapiens
129 SUN2 25777
Proximity Label-MS Homo sapiens
130 ATF6  
Proximity Label-MS Homo sapiens
131 TRPM7 54822
Proximity Label-MS Homo sapiens
132 TAPT1 202018
Proximity Label-MS Homo sapiens
133 SYNE1 23345
Proximity Label-MS Homo sapiens
134 RPL13A 23521
Affinity Capture-MS Homo sapiens
135 MIA3 375056
Proximity Label-MS Homo sapiens
136 HM13 81502
Proximity Label-MS Homo sapiens
137 RPL36 25873
Affinity Capture-MS Homo sapiens
138 P4HB 5034
Proximity Label-MS Homo sapiens
139 TNFRSF10C  
Two-hybrid Homo sapiens
140 RPN1 6184
Proximity Label-MS Homo sapiens
141 GANAB 23193
Proximity Label-MS Homo sapiens
142 ACBD5 91452
Proximity Label-MS Homo sapiens
143 STX5 6811
Proximity Label-MS Homo sapiens
144 PBXIP1 57326
Proximity Label-MS Homo sapiens
145 TSPAN31 6302
Affinity Capture-MS Homo sapiens
146 RRP1B 23076
Affinity Capture-MS Homo sapiens
147 TMEM131  
Proximity Label-MS Homo sapiens
148 AHCTF1 25909
Proximity Label-MS Homo sapiens
149 SERPINA3 12
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
150 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
151 PPM1G 5496
Affinity Capture-MS Homo sapiens
152 DRG2 1819
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
153 EIF2B4 8890
Affinity Capture-MS Homo sapiens
154 EMC3 55831
Proximity Label-MS Homo sapiens
155 ACBD3 64746
Proximity Label-MS Homo sapiens
156 TEX2 55852
Proximity Label-MS Homo sapiens
157 REEP5 7905
Proximity Label-MS Homo sapiens
158 PCDHA8  
Affinity Capture-MS Homo sapiens
159 MAP2K1 5604
Proximity Label-MS Homo sapiens
160 SEC63 11231
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
161 PKMYT1  
Proximity Label-MS Homo sapiens
162 HSPA5 3309
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
163 ANO6 196527
Proximity Label-MS Homo sapiens
164 TSR1 55720
Proximity Label-MS Homo sapiens
165 SMCR8 140775
Proximity Label-MS Homo sapiens
166 TMEM199  
Proximity Label-MS Homo sapiens
167 PTDSS1 9791
Proximity Label-MS Homo sapiens
168 PREB 10113
Proximity Label-MS Homo sapiens
169 HADHB 3032
Affinity Capture-MS Homo sapiens
170 IL31  
Affinity Capture-MS Homo sapiens
171 RPN2 6185
Proximity Label-MS Homo sapiens
172 SURF4 6836
Proximity Label-MS Homo sapiens
173 CANX 821
Proximity Label-MS Homo sapiens
174 RPL7A 6130
Affinity Capture-MS Homo sapiens
175 LBR 3930
Proximity Label-MS Homo sapiens
176 EMC8 10328
Proximity Label-MS Homo sapiens
177 LRRC59 55379
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
178 LMNB1 4001
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
179 ADCY9 115
Proximity Label-MS Homo sapiens
180 GPR89B 51463
Proximity Label-MS Homo sapiens
181 ZNF622 90441
Proximity Label-MS Homo sapiens
182 HMOX2 3163
Proximity Label-MS Homo sapiens
183 EMC10 284361
Proximity Label-MS Homo sapiens
184 RPL21 6144
Affinity Capture-MS Homo sapiens
185 LMNB2 84823
Proximity Label-MS Homo sapiens
186 DDX60 55601
Affinity Capture-MS Homo sapiens
187 BCAP31 10134
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
188 HSD3B7 80270
Proximity Label-MS Homo sapiens
189 ATP6AP2 10159
Proximity Label-MS Homo sapiens
190 STIM1 6786
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
191 RMND1 55005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 RPL3 6122
Affinity Capture-MS Homo sapiens
193 FKBP8 23770
Proximity Label-MS Homo sapiens
194 IGF2R 3482
Proximity Label-MS Homo sapiens
195 SSFA2 6744
Proximity Label-MS Homo sapiens
196 SYNE2 23224
Proximity Label-MS Homo sapiens
197 FAM63B 54629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
198 RPL7 6129
Affinity Capture-MS Homo sapiens
199 NOP2 4839
Affinity Capture-MS Homo sapiens
200 PLXNB2 23654
Proximity Label-MS Homo sapiens
201 GRAMD1A  
Proximity Label-MS Homo sapiens
202 DCAF8 50717
Affinity Capture-MS Homo sapiens
203 RPL28 6158
Affinity Capture-MS Homo sapiens
204 SAAL1 113174
Affinity Capture-MS Homo sapiens
205 SOAT1 6646
Proximity Label-MS Homo sapiens
206 ACSL3 2181
Proximity Label-MS Homo sapiens
207 XPR1 9213
Proximity Label-MS Homo sapiens
208 CHCHD3 54927
Proximity Label-MS Homo sapiens
209 LTV1  
Proximity Label-MS Homo sapiens
210 ATP2A1 487
Proximity Label-MS Homo sapiens
211 NOTCH2 4853
Proximity Label-MS Homo sapiens
212 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
213 SCGB2A2  
Affinity Capture-MS Homo sapiens
214 FAM134B 54463
Proximity Label-MS Homo sapiens
215 TTC17 55761
Proximity Label-MS Homo sapiens
216 AQP3  
Affinity Capture-MS Homo sapiens
217 RPL12 6136
Affinity Capture-MS Homo sapiens
218 STT3B 201595
Proximity Label-MS Homo sapiens
219 CLPTM1 1209
Proximity Label-MS Homo sapiens
220 VRK2 7444
Proximity Label-MS Homo sapiens
221 CORO1A 11151
Affinity Capture-MS Homo sapiens
222 DNAJC3 5611
Affinity Capture-MS Homo sapiens
223 DHFRL1  
Proximity Label-MS Homo sapiens
224 RPL18 6141
Affinity Capture-MS Homo sapiens