Gene description for AHCTF1
Gene name AT hook containing transcription factor 1
Gene symbol AHCTF1
Other names/aliases ELYS
MST108
MSTP108
TMBS62
Species Homo sapiens
 Database cross references - AHCTF1
ExoCarta ExoCarta_25909
Vesiclepedia VP_25909
Entrez Gene 25909
HGNC 24618
MIM 610853
UniProt Q8WYP5  
 AHCTF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for AHCTF1
Molecular Function
    DNA binding GO:0003677 IEA
Biological Process
    nucleocytoplasmic transport GO:0006913 NAS
    protein transport GO:0015031 IEA
    regulation of cytokinesis GO:0032465 IMP
    mRNA transport GO:0051028 IEA
    nuclear pore complex assembly GO:0051292 IMP
    cell division GO:0051301 IEA
Subcellular Localization
    kinetochore GO:0000776 IDA
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    nuclear envelope GO:0005635 IDA
    nuclear pore GO:0005643 IDA
    nuclear pore GO:0005643 NAS
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    nuclear matrix GO:0016363 IEA
    nuclear body GO:0016604 IDA
    nuclear pore outer ring GO:0031080 IDA
    nuclear membrane GO:0031965 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified AHCTF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for AHCTF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 AFG3L2 10939
Affinity Capture-MS Homo sapiens
4 FMOD 2331
Affinity Capture-MS Homo sapiens
5 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LAMP3  
Affinity Capture-MS Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 RIT1 6016
Negative Genetic Homo sapiens
9 TRIM66  
Affinity Capture-MS Homo sapiens
10 ACTC1 70
Proximity Label-MS Homo sapiens
11 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 GPR182  
Affinity Capture-MS Homo sapiens
14 PTPN1 5770
Proximity Label-MS Homo sapiens
15 DERL1 79139
Proximity Label-MS Homo sapiens
16 NUP160 23279
Two-hybrid Homo sapiens
17 APEX1 328
Proximity Label-MS Homo sapiens
18 HLA-B 3106
Affinity Capture-MS Homo sapiens
19 NPM1 4869
Affinity Capture-MS Homo sapiens
20 HMGA1 3159
Affinity Capture-MS Homo sapiens
21 PMEL 6490
Affinity Capture-MS Homo sapiens
22 TACSTD2 4070
Affinity Capture-MS Homo sapiens
23 EED  
Affinity Capture-MS Homo sapiens
24 SLC25A46 91137
Proximity Label-MS Homo sapiens
25 AIFM1 9131
Affinity Capture-MS Homo sapiens
26 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FAF2 23197
Affinity Capture-MS Homo sapiens
28 KIF14 9928
Affinity Capture-MS Homo sapiens
29 NUP155 9631
Proximity Label-MS Homo sapiens
30 CHMP4C 92421
Affinity Capture-MS Homo sapiens
31 PPP1CA 5499
Reconstituted Complex Homo sapiens
32 CD70 970
Affinity Capture-MS Homo sapiens
33 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
34 EMD 2010
Proximity Label-MS Homo sapiens
35 ITGAD  
Affinity Capture-MS Homo sapiens
36 TNFSF8  
Affinity Capture-MS Homo sapiens
37 VSIG4  
Affinity Capture-MS Homo sapiens
38 CBX3 11335
Proximity Label-MS Homo sapiens
39 CHMP4B 128866
Affinity Capture-MS Homo sapiens
40 SGCA  
Affinity Capture-MS Homo sapiens
41 DTX2 113878
Proximity Label-MS Homo sapiens
42 S100B 6285
Affinity Capture-MS Homo sapiens
43 RPA4  
Proximity Label-MS Homo sapiens
44 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
45 RPL29 6159
Cross-Linking-MS (XL-MS) Homo sapiens
46 FGFBP1 9982
Affinity Capture-MS Homo sapiens
47 NUMA1 4926
Affinity Capture-MS Homo sapiens
48 NUP37 79023
Affinity Capture-MS Homo sapiens
49 HIST1H3A 8350
Proximity Label-MS Homo sapiens
50 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 FURIN 5045
Affinity Capture-MS Homo sapiens
52 Nup98  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
53 LMNB1 4001
Proximity Label-MS Homo sapiens
54 SIRT7  
Affinity Capture-MS Homo sapiens
55 LRRC25  
Affinity Capture-MS Homo sapiens
56 MEX3A  
Affinity Capture-RNA Homo sapiens
57 PDGFRA 5156
Affinity Capture-MS Homo sapiens
58 MGARP  
Affinity Capture-MS Homo sapiens
59 CUL3 8452
Affinity Capture-MS Homo sapiens
60 KLRD1  
Affinity Capture-MS Homo sapiens
61 NUP43 348995
Proximity Label-MS Homo sapiens
62 NXF1 10482
Affinity Capture-RNA Homo sapiens
63 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
64 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
65 MYC  
Affinity Capture-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 HIST1H2BA 255626
Cross-Linking-MS (XL-MS) Homo sapiens
68 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
70 RNF43  
Proximity Label-MS Homo sapiens
71 STK16 8576
Affinity Capture-MS Homo sapiens
72 NUP133 55746
Co-fractionation Homo sapiens
73 TP53 7157
Affinity Capture-MS Homo sapiens
74 OPALIN  
Affinity Capture-MS Homo sapiens
75 CLEC2D  
Affinity Capture-MS Homo sapiens
76 TNFSF14  
Affinity Capture-MS Homo sapiens
77 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
78 MAMDC4  
Affinity Capture-MS Homo sapiens
79 SHMT2 6472
Affinity Capture-RNA Homo sapiens
80 NUP35 129401
Proximity Label-MS Homo sapiens
81 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 KIR2DS2  
Affinity Capture-MS Homo sapiens
83 NUP50 10762
Proximity Label-MS Homo sapiens
84 MOV10 4343
Affinity Capture-RNA Homo sapiens
85 XRCC6 2547
Proximity Label-MS Homo sapiens
86 DNAJC1 64215
Proximity Label-MS Homo sapiens
87 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
88 Nup107  
Affinity Capture-MS Mus musculus
89 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
90 PCDHB3  
Affinity Capture-MS Homo sapiens
91 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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