Gene description for HLA-B
Gene name major histocompatibility complex, class I, B
Gene symbol HLA-B
Other names/aliases AS
HLAB
SPDA1
Species Homo sapiens
 Database cross references - HLA-B
ExoCarta ExoCarta_3106
Vesiclepedia VP_3106
Entrez Gene 3106
HGNC 4932
MIM 142830
UniProt P01889  
 HLA-B identified in sEVs derived from the following tissue/cell type
B cells 12519789    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 19837982    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 22723089    
Saliva 19199708    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for HLA-B
Molecular Function
    signaling receptor binding GO:0005102 IBA
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 IDA
    TAP binding GO:0046977 IDA
    TAP binding GO:0046977 IDA
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 IBA
    positive regulation of T cell mediated cytotoxicity GO:0001916 IDA
    adaptive immune response GO:0002250 IEA
    antigen processing and presentation of endogenous peptide antigen via MHC class Ib GO:0002476 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent GO:0002486 IBA
    antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent GO:0002486 IDA
    regulation of T cell anergy GO:0002667 IMP
    defense response GO:0006952 TAS
    immune response GO:0006955 IBA
    immune response GO:0006955 IMP
    immune response GO:0006955 NAS
    detection of bacterium GO:0016045 IMP
    regulation of interleukin-12 production GO:0032655 IMP
    regulation of interleukin-6 production GO:0032675 IMP
    protection from natural killer cell mediated cytotoxicity GO:0042270 IDA
    innate immune response GO:0045087 IEA
    regulation of dendritic cell differentiation GO:2001198 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    extracellular space GO:0005615 IBA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    cell surface GO:0009986 HDA
    cell surface GO:0009986 IDA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 TAS
    secretory granule membrane GO:0030667 TAS
    phagocytic vesicle membrane GO:0030670 TAS
    early endosome membrane GO:0031901 TAS
    MHC class I protein complex GO:0042612 IDA
    recycling endosome membrane GO:0055038 TAS
    extracellular exosome GO:0070062 HDA
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
 Experiment description of studies that identified HLA-B in sEVs
1
Experiment ID 11
MISEV standards
✘ Biophysical techniques
✔
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
2
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
5
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
6
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
10
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
23
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
24
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
28
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
29
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HLA-B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 INTS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DOPEY2 9980
Affinity Capture-MS Homo sapiens
4 RHOF 54509
Affinity Capture-MS Homo sapiens
5 MON2 23041
Affinity Capture-MS Homo sapiens
6 ATR  
Affinity Capture-MS Homo sapiens
7 PCK1 5105
Affinity Capture-MS Homo sapiens
8 CHRNA4  
Affinity Capture-MS Homo sapiens
9 C1orf27 54953
Affinity Capture-MS Homo sapiens
10 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NUP188 23511
Affinity Capture-MS Homo sapiens
12 ABCC1 4363
Affinity Capture-MS Homo sapiens
13 ADRB2  
Affinity Capture-MS Homo sapiens
14 PAICS 10606
Affinity Capture-MS Homo sapiens
15 TUBB8 347688
Affinity Capture-MS Homo sapiens
16 ATP12A 479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SPNS1 83985
Affinity Capture-MS Homo sapiens
18 SLC27A6  
Affinity Capture-MS Homo sapiens
19 INTS1 26173
Affinity Capture-MS Homo sapiens
20 VPS51 738
Affinity Capture-MS Homo sapiens
21 LTN1 26046
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 GCN1L1 10985
Affinity Capture-MS Homo sapiens
23 C1orf112  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PI4KA 5297
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SLC39A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SLC5A6 8884
Affinity Capture-MS Homo sapiens
27 TELO2 9894
Affinity Capture-MS Homo sapiens
28 INTS8  
Affinity Capture-MS Homo sapiens
29 ADAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SLC38A5 92745
Affinity Capture-MS Homo sapiens
31 RUVBL1 8607
Affinity Capture-MS Homo sapiens
32 TRIM28 10155
Affinity Capture-MS Homo sapiens
33 B3GNT2 10678
Affinity Capture-MS Homo sapiens
34 LPGAT1 9926
Affinity Capture-MS Homo sapiens
35 KIF23 9493
Affinity Capture-MS Homo sapiens
36 VARS 7407
Affinity Capture-MS Homo sapiens
37 MDN1 23195
Affinity Capture-MS Homo sapiens
38 HLA-B 3106
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 SMPD4 55627
Affinity Capture-MS Homo sapiens
40 RHBDD2  
Affinity Capture-MS Homo sapiens
41 LILRB1  
Reconstituted Complex Homo sapiens
42 ERAP1 51752
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KLRC1  
Affinity Capture-MS Homo sapiens
44 AHCY 191
Affinity Capture-MS Homo sapiens
45 FANCD2  
Affinity Capture-MS Homo sapiens
46 DNAJC16  
Affinity Capture-MS Homo sapiens
47 TNFSF9  
Affinity Capture-MS Homo sapiens
48 WDFY3 23001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 UFSP2 55325
Affinity Capture-MS Homo sapiens
50 PRKDC 5591
Affinity Capture-MS Homo sapiens
51 BTAF1 9044
Affinity Capture-MS Homo sapiens
52 ACSL1 2180
Affinity Capture-MS Homo sapiens
53 RAB35 11021
Affinity Capture-MS Homo sapiens
54 ARFGEF2 10564
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ITM2B 9445
Affinity Capture-MS Homo sapiens
56 FAF2 23197
Affinity Capture-MS Homo sapiens
57 PLS3 5358
Affinity Capture-MS Homo sapiens
58 ARHGEF4  
Two-hybrid Homo sapiens
59 NTSR1  
Affinity Capture-MS Homo sapiens
60 HLA-G 3135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KIAA0196 9897
Affinity Capture-MS Homo sapiens
62 ATP1A3 478
Affinity Capture-MS Homo sapiens
63 TAZ  
Affinity Capture-MS Homo sapiens
64 CHMP4C 92421
Affinity Capture-MS Homo sapiens
65 PDK1 5163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 AP3M1 26985
Affinity Capture-MS Homo sapiens
67 SLC1A3 6507
Affinity Capture-MS Homo sapiens
68 SDHA 6389
Affinity Capture-MS Homo sapiens
69 Tap2  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
70 TMED6  
Affinity Capture-MS Homo sapiens
71 TFB2M  
Affinity Capture-MS Homo sapiens
72 IRAK1  
Affinity Capture-MS Homo sapiens
73 CTDP1 9150
Affinity Capture-MS Homo sapiens
74 ANTXR1 84168
Affinity Capture-MS Homo sapiens
75 PARK2  
Affinity Capture-MS Homo sapiens
76 CYB5D2  
Affinity Capture-MS Homo sapiens
77 ERBB3 2065
Affinity Capture-MS Homo sapiens
78 TUBA1A 7846
Affinity Capture-MS Homo sapiens
79 CLTCL1 8218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 HTT 3064
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RNF213 57674
Affinity Capture-MS Homo sapiens
82 CD8A 925
Reconstituted Complex Homo sapiens
83 PMAIP1  
Affinity Capture-MS Homo sapiens
84 C16orf58 64755
Affinity Capture-MS Homo sapiens
85 ST6GAL1 6480
Affinity Capture-MS Homo sapiens
86 NEK4  
Affinity Capture-MS Homo sapiens
87 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 IL27RA 9466
Affinity Capture-MS Homo sapiens
89 XPOT 11260
Affinity Capture-MS Homo sapiens
90 SGSM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 PATE1  
Affinity Capture-MS Homo sapiens
92 ITFG1 81533
Affinity Capture-MS Homo sapiens
93 AHCTF1 25909
Affinity Capture-MS Homo sapiens
94 PTPRO 5800
Affinity Capture-MS Homo sapiens
95 TMEM70  
Affinity Capture-MS Homo sapiens
96 TAP2 6891
Affinity Capture-MS Homo sapiens
97 ABCB7 22
Affinity Capture-MS Homo sapiens
98 VCP 7415
Affinity Capture-MS Homo sapiens
99 Tap1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-MS Rattus norvegicus
100 HEATR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 CDS2 8760
Affinity Capture-MS Homo sapiens
102 HSPA5 3309
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
103 WDR11 55717
Affinity Capture-MS Homo sapiens
104 SLC35E1 79939
Affinity Capture-MS Homo sapiens
105 CLN3 1201
Affinity Capture-MS Homo sapiens
106 TMEM30A 55754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 CXCR4 7852
Affinity Capture-Western Homo sapiens
108 PCDH20  
Affinity Capture-MS Homo sapiens
109 CANX 821
Affinity Capture-MS Homo sapiens
110 CD151 977
Affinity Capture-MS Homo sapiens
111 NDUFA9 4704
Co-fractionation Homo sapiens
112 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 NCAPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 ARFGEF1 10565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 RAB29 8934
Affinity Capture-MS Homo sapiens
116 CYB5R3 1727
Co-fractionation Homo sapiens
117 TOMM22 56993
Affinity Capture-MS Homo sapiens
118 EGFR 1956
Affinity Capture-MS Homo sapiens
119 KLRD1  
Affinity Capture-MS Homo sapiens
120 GBF1 8729
Affinity Capture-MS Homo sapiens
121 TTC27 55622
Affinity Capture-MS Homo sapiens
122 CYFIP2 26999
Affinity Capture-MS Homo sapiens
123 RIPK4  
Affinity Capture-MS Homo sapiens
124 SLC39A10 57181
Affinity Capture-MS Homo sapiens
125 AURKB 9212
Affinity Capture-MS Homo sapiens
126 TUBA4A 7277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 FANCA  
Affinity Capture-MS Homo sapiens
128 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
129 STAT1 6772
Two-hybrid Homo sapiens
130 FAM91A1 157769
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 HLA-F 3134
Affinity Capture-MS Homo sapiens
132 PSCA 8000
Affinity Capture-MS Homo sapiens
133 TMEM246 84302
Affinity Capture-MS Homo sapiens
134 MROH1 727957
Affinity Capture-MS Homo sapiens
135 CDC45  
Affinity Capture-MS Homo sapiens
136 MED23 9439
Affinity Capture-MS Homo sapiens
137 ABCA2 20
Affinity Capture-MS Homo sapiens
138 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
139 KIAA0368 23392
Affinity Capture-MS Homo sapiens
140 CSDE1 7812
Cross-Linking-MS (XL-MS) Homo sapiens
141 MSN 4478
Affinity Capture-MS Homo sapiens
142 HEATR1 55127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 HLA-A 3105
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 INTS5 80789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 GGCX 2677
Affinity Capture-MS Homo sapiens
146 HUWE1 10075
Affinity Capture-MS Homo sapiens
147 LOC100507703  
Affinity Capture-MS Homo sapiens
148 PFAS 5198
Affinity Capture-MS Homo sapiens
149 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
150 RTTN  
Affinity Capture-MS Homo sapiens
151 MR1  
Affinity Capture-MS Homo sapiens
152 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
153 ABCB6 10058
Affinity Capture-MS Homo sapiens
154 NUP205 23165
Affinity Capture-MS Homo sapiens
155 SIAE 54414
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 FASTKD1  
Affinity Capture-MS Homo sapiens
157 AGPAT9  
Affinity Capture-MS Homo sapiens
158 TTC28 23331
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 IGHD  
Affinity Capture-MS Homo sapiens
160 FCGRT 2217
Affinity Capture-MS Homo sapiens
161 ADAM33 80332
Affinity Capture-MS Homo sapiens
162 ASIC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 RIF1  
Affinity Capture-MS Homo sapiens
164 CD1B 910
Affinity Capture-MS Homo sapiens
165 WNT4  
Affinity Capture-MS Homo sapiens
166 EDEM1  
Affinity Capture-Western Homo sapiens
167 CD1A 909
Affinity Capture-MS Homo sapiens
168 FANCI 55215
Affinity Capture-MS Homo sapiens
169 DDX58 23586
Affinity Capture-RNA Homo sapiens
170 TARS 6897
Affinity Capture-MS Homo sapiens
171 STARD3 10948
Affinity Capture-MS Homo sapiens
172 UBE3C 9690
Affinity Capture-MS Homo sapiens
173 KIR3DL3  
Affinity Capture-MS Homo sapiens
174 OGFOD3 79701
Affinity Capture-MS Homo sapiens
175 TAPBP 6892
Affinity Capture-MS Homo sapiens
176 KIR3DS1  
Phenotypic Enhancement Homo sapiens
177 UXS1 80146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
178 SLC25A4 291
Affinity Capture-MS Homo sapiens
179 C16orf72 29035
Affinity Capture-MS Homo sapiens
180 TRIM31  
Affinity Capture-MS Homo sapiens
181 B2M 567
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
182 NCEH1 57552
Affinity Capture-MS Homo sapiens
183 TRIB3  
Two-hybrid Homo sapiens
184 AGPAT6 137964
Affinity Capture-MS Homo sapiens
185 HLA-H 3136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 IPO13 9670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
187 EZR 7430
Affinity Capture-MS Homo sapiens
188 SLC20A1 6574
Affinity Capture-MS Homo sapiens
189 C9orf72  
Affinity Capture-MS Homo sapiens
190 LILRB2  
Reconstituted Complex Homo sapiens
191 PSMD1 5707
Affinity Capture-MS Homo sapiens
192 SLC1A1 6505
Affinity Capture-MS Homo sapiens
193 PTCH1  
Affinity Capture-MS Homo sapiens
194 TRAV20  
Affinity Capture-MS Homo sapiens
195 CLEC4E  
Affinity Capture-MS Homo sapiens
196 CLN6  
Affinity Capture-MS Homo sapiens
197 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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