Gene description for CD8A
Gene name CD8a molecule
Gene symbol CD8A
Other names/aliases CD8
Leu2
MAL
p32
Species Homo sapiens
 Database cross references - CD8A
ExoCarta ExoCarta_925
Vesiclepedia VP_925
Entrez Gene 925
HGNC 1706
MIM 186910
UniProt P01732  
 CD8A identified in sEVs derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for CD8A
Molecular Function
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 NAS
    protein kinase binding GO:0019901 IEA
    MHC class I protein complex binding GO:0023024 IDA
    MHC class I protein binding GO:0042288 NAS
Biological Process
    adaptive immune response GO:0002250 NAS
    T cell mediated immunity GO:0002456 IBA
    immune response GO:0006955 NAS
    cell surface receptor signaling pathway GO:0007166 IBA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 NAS
    antigen processing and presentation GO:0019882 NAS
    T cell activation GO:0042110 IDA
    T cell activation GO:0042110 ISS
    T cell activation GO:0042110 NAS
    cytotoxic T cell differentiation GO:0045065 IBA
    T cell receptor signaling pathway GO:0050852 IDA
    T cell receptor signaling pathway GO:0050852 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    T cell receptor complex GO:0042101 NAS
    receptor complex GO:0043235 IPI
    receptor complex GO:0043235 ISO
    plasma membrane raft GO:0044853 IDA
 Experiment description of studies that identified CD8A in sEVs
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CD8A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK1 983
Two-hybrid Homo sapiens
2 HSPA5 3309
Affinity Capture-MS Homo sapiens
3 CD59 966
Reconstituted Complex Homo sapiens
4 HLA-A 3105
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
5 CD8A 925
Co-localization Homo sapiens
Co-crystal Structure Homo sapiens
Co-localization Homo sapiens
Co-crystal Structure Homo sapiens
6 PTPRC 5788
FRET Homo sapiens
7 NPTX1  
Affinity Capture-MS Homo sapiens
8 ANKRD32  
Affinity Capture-MS Homo sapiens
9 CD3D 915
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
10 TP53 7157
Negative Genetic Homo sapiens
11 LNX1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
12 LNX2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
13 HLA-G 3135
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 CD8B 926
Co-localization Homo sapiens
15 HLA-B 3106
Reconstituted Complex Homo sapiens
16 COPA 1314
Affinity Capture-Western Homo sapiens
17 HLA-C 3107
Reconstituted Complex Homo sapiens
18 CACYBP 27101
Two-hybrid Homo sapiens
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