Gene description for NUP188
Gene name nucleoporin 188kDa
Gene symbol NUP188
Other names/aliases KIAA0169
hNup188
Species Homo sapiens
 Database cross references - NUP188
ExoCarta ExoCarta_23511
Vesiclepedia VP_23511
Entrez Gene 23511
HGNC 17859
MIM 615587
UniProt Q5SRE5  
 NUP188 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NUP188
Molecular Function
    structural constituent of nuclear pore GO:0017056 IBA
Biological Process
    RNA export from nucleus GO:0006405 IBA
    protein import into nucleus GO:0006606 IBA
    protein import into nucleus GO:0006606 IMP
    nucleocytoplasmic transport GO:0006913 NAS
    mRNA transport GO:0051028 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 NAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    nuclear pore inner ring GO:0044611 IBA
 Experiment description of studies that identified NUP188 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NUP188
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
2 COIL  
Proximity Label-MS Homo sapiens
3 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
4 USP48  
Co-fractionation Homo sapiens
5 NUP62 23636
Affinity Capture-MS Homo sapiens
6 KPNB1 3837
Affinity Capture-MS Homo sapiens
7 NUPL1 9818
Affinity Capture-MS Homo sapiens
8 Ccdc12  
Affinity Capture-MS Mus musculus
9 CENPA  
Proximity Label-MS Homo sapiens
10 HLA-B 3106
Affinity Capture-MS Homo sapiens
11 NUP153 9972
Affinity Capture-MS Homo sapiens
12 PRC1 9055
Affinity Capture-MS Homo sapiens
13 NUP93 9688
Co-fractionation Homo sapiens
14 POLR1E  
Proximity Label-MS Homo sapiens
15 OBSL1 23363
Affinity Capture-MS Homo sapiens
16 NR3C1 2908
Proximity Label-MS Homo sapiens
17 ITGA4 3676
Affinity Capture-MS Homo sapiens
18 EED  
Affinity Capture-MS Homo sapiens
19 SLX4  
Affinity Capture-MS Homo sapiens
20 VCAM1 7412
Affinity Capture-MS Homo sapiens
21 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DVL2 1856
Affinity Capture-MS Homo sapiens
23 NUP155 9631
Proximity Label-MS Homo sapiens
24 KRT8 3856
Proximity Label-MS Homo sapiens
25 Nup188  
Affinity Capture-MS Mus musculus
26 NDUFS4 4724
Co-fractionation Homo sapiens
27 Rcc1  
Affinity Capture-MS Mus musculus
28 Ypel5  
Affinity Capture-MS Mus musculus
29 COMTD1 118881
Affinity Capture-MS Homo sapiens
30 PTP4A3  
Affinity Capture-MS Homo sapiens
31 RAN 5901
Affinity Capture-MS Homo sapiens
32 NTRK1 4914
Affinity Capture-MS Homo sapiens
33 RPL31 6160
Proximity Label-MS Homo sapiens
34 HUWE1 10075
Affinity Capture-MS Homo sapiens
35 TRIM66  
Affinity Capture-MS Homo sapiens
36 DHFRL1  
Proximity Label-MS Homo sapiens
37 Ube2i  
Affinity Capture-MS Mus musculus
38 CD3EAP  
Proximity Label-MS Homo sapiens
39 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
40 Nup98  
Affinity Capture-MS Mus musculus
41 XPO1 7514
Affinity Capture-MS Homo sapiens
42 EIF6 3692
Proximity Label-MS Homo sapiens
43 CD79B 974
Affinity Capture-MS Homo sapiens
44 ECT2 1894
Affinity Capture-MS Homo sapiens
45 SIRT7  
Affinity Capture-MS Homo sapiens
46 IQCB1  
Affinity Capture-MS Homo sapiens
47 KCMF1 56888
Affinity Capture-MS Homo sapiens
48 SPC24 147841
Affinity Capture-MS Homo sapiens
49 CUL3 8452
Affinity Capture-MS Homo sapiens
50 Rrbp1  
Affinity Capture-MS Mus musculus
51 NUP214 8021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DMTN  
Affinity Capture-MS Homo sapiens
53 NXF1 10482
Affinity Capture-MS Homo sapiens
54 ICK  
Affinity Capture-MS Homo sapiens
55 NEFM 4741
Proximity Label-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 SEH1L 81929
Affinity Capture-MS Homo sapiens
58 HAX1  
Proximity Label-MS Homo sapiens
59 CSDE1 7812
Co-fractionation Homo sapiens
60 PEX3 8504
Proximity Label-MS Homo sapiens
61 Psmd6 66413
Affinity Capture-MS Mus musculus
62 AKAP1 8165
Proximity Label-MS Homo sapiens
63 FLOT1 10211
Proximity Label-MS Homo sapiens
64 CCDC8  
Affinity Capture-MS Homo sapiens
65 HIPK4  
Affinity Capture-MS Homo sapiens
66 KRT18 3875
Proximity Label-MS Homo sapiens
67 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
68 FASN 2194
Negative Genetic Homo sapiens
69 SPTAN1 6709
Co-fractionation Homo sapiens
70 RAE1 8480
Affinity Capture-MS Homo sapiens
71 SIRT6  
Affinity Capture-MS Homo sapiens
72 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 MEN1 4221
Affinity Capture-MS Homo sapiens
74 G3BP1 10146
Affinity Capture-MS Homo sapiens
75 DLK1  
Affinity Capture-MS Homo sapiens
76 NUP35 129401
Proximity Label-MS Homo sapiens
77 SLAMF1 6504
Affinity Capture-MS Homo sapiens
78 NIFK 84365
Proximity Label-MS Homo sapiens
79 Nup214  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
80 NUP107 57122
Affinity Capture-MS Homo sapiens
81 RPS24 6229
Co-fractionation Homo sapiens
82 C16orf72 29035
Affinity Capture-MS Homo sapiens
83 CIT 11113
Affinity Capture-MS Homo sapiens
84 Ranbp2  
Affinity Capture-MS Mus musculus
85 BRD1 23774
Affinity Capture-MS Homo sapiens
86 HSPA4L 22824
Co-fractionation Homo sapiens
87 C9orf72  
Affinity Capture-MS Homo sapiens
88 AR 367
Affinity Capture-MS Homo sapiens
89 Nup107  
Affinity Capture-MS Mus musculus
90 UBA6 55236
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NUP188 is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prophase TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope (NE) Reassembly TAS Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation TAS Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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