Gene description for SEH1L
Gene name SEH1-like (S. cerevisiae)
Gene symbol SEH1L
Other names/aliases SEC13L
SEH1A
SEH1B
Seh1
Species Homo sapiens
 Database cross references - SEH1L
ExoCarta ExoCarta_81929
Vesiclepedia VP_81929
Entrez Gene 81929
HGNC 30379
MIM 609263
UniProt Q96EE3  
 SEH1L identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for SEH1L
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
Biological Process
    nucleocytoplasmic transport GO:0006913 NAS
    nuclear pore organization GO:0006999 IMP
    mitotic metaphase chromosome alignment GO:0007080 IMP
    protein transport GO:0015031 IEA
    protein-containing complex localization GO:0031503 IMP
    cellular response to nutrient levels GO:0031669 IDA
    cellular response to amino acid starvation GO:0034198 IBA
    cellular response to amino acid starvation GO:0034198 IMP
    defense response to Gram-positive bacterium GO:0050830 IEA
    mRNA transport GO:0051028 IEA
    cell division GO:0051301 IEA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IMP
    negative regulation of TORC1 signaling GO:1904262 NAS
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IDA
    positive regulation of TORC1 signaling GO:1904263 IMP
    positive regulation of TORC1 signaling GO:1904263 NAS
Subcellular Localization
    kinetochore GO:0000776 IDA
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 NAS
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    cytosol GO:0005829 TAS
    nuclear pore outer ring GO:0031080 IBA
    nuclear pore outer ring GO:0031080 IDA
    nuclear pore outer ring GO:0031080 NAS
    Seh1-associated complex GO:0035859 IBA
    GATOR2 complex GO:0061700 IDA
    GATOR2 complex GO:0061700 NAS
 Experiment description of studies that identified SEH1L in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SEH1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAM131B  
Affinity Capture-MS Homo sapiens
2 NPRL2  
Affinity Capture-Western Homo sapiens
3 GATSL2  
Co-fractionation Homo sapiens
4 BUB1  
Affinity Capture-MS Homo sapiens
5 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
6 NUP188 23511
Affinity Capture-MS Homo sapiens
7 RPA2 6118
Affinity Capture-MS Homo sapiens
8 KPNB1 3837
Affinity Capture-MS Homo sapiens
9 DNAJB2 3300
Proximity Label-MS Homo sapiens
10 BGLAP  
Affinity Capture-MS Homo sapiens
11 MTMR14 64419
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 TAB1 10454
Affinity Capture-MS Homo sapiens
14 CCT4 10575
Affinity Capture-MS Homo sapiens
15 SEMG2 6407
Affinity Capture-MS Homo sapiens
16 DNAJB3 414061
Proximity Label-MS Homo sapiens
17 AZGP1 563
Affinity Capture-MS Homo sapiens
18 CCDC102B  
Affinity Capture-MS Homo sapiens
19 NUP160 23279
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
20 PARK2  
Affinity Capture-MS Homo sapiens
21 DCTN4 51164
Co-fractionation Homo sapiens
22 NUP153 9972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 COPS5 10987
Affinity Capture-MS Homo sapiens
24 ACPP 55
Affinity Capture-MS Homo sapiens
25 CCT3 7203
Affinity Capture-MS Homo sapiens
26 NUP93 9688
Affinity Capture-MS Homo sapiens
27 Cenpe  
Affinity Capture-MS Mus musculus
28 PLA2G10  
Affinity Capture-MS Homo sapiens
29 ATG16L1 55054
Affinity Capture-MS Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 KPTN  
Affinity Capture-Western Homo sapiens
32 HAT1 8520
Affinity Capture-MS Homo sapiens
33 NUP155 9631
Affinity Capture-MS Homo sapiens
34 CXCL1 2919
Affinity Capture-MS Homo sapiens
35 PPP1CA 5499
Reconstituted Complex Homo sapiens
36 POM121C  
Affinity Capture-MS Homo sapiens
37 DNAJB8 165721
Proximity Label-MS Homo sapiens
38 Rcc1  
Affinity Capture-MS Mus musculus
39 QPRT 23475
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SZT2 23334
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
41 CCT5 22948
Affinity Capture-MS Homo sapiens
42 UBE2I 7329
Affinity Capture-MS Homo sapiens
43 MSMB  
Affinity Capture-MS Homo sapiens
44 NELL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 Ube2i  
Affinity Capture-MS Mus musculus
46 CEBPA  
Protein-peptide Homo sapiens
47 NUP210 23225
Affinity Capture-MS Homo sapiens
48 Nup155  
Affinity Capture-MS Mus musculus
49 NUP37 79023
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
50 Nup98  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
51 USP32P2  
Affinity Capture-MS Homo sapiens
52 SEC13 6396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 GLE1 2733
Affinity Capture-MS Homo sapiens
54 Kifc1  
Affinity Capture-MS Mus musculus
55 CRYAB 1410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 LAMP1 3916
Affinity Capture-Western Homo sapiens
57 NUP88 4927
Affinity Capture-MS Homo sapiens
58 PPP1R37 284352
Affinity Capture-MS Homo sapiens
59 EGFR 1956
Negative Genetic Homo sapiens
60 NUP43 348995
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
61 AAAS 8086
Affinity Capture-MS Homo sapiens
62 NUP214 8021
Affinity Capture-MS Homo sapiens
63 Ranbp2  
Affinity Capture-MS Mus musculus
64 NXF1 10482
Affinity Capture-MS Homo sapiens
65 SEMG1 6406
Affinity Capture-MS Homo sapiens
66 WDR24  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
67 Nup107  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
68 HECW2  
Affinity Capture-MS Homo sapiens
69 KLK3  
Affinity Capture-MS Homo sapiens
70 Sesn2  
Affinity Capture-MS Mus musculus
71 SAP25  
Affinity Capture-MS Homo sapiens
72 ATP6V0A1 535
Two-hybrid Homo sapiens
73 TMEM209 84928
Affinity Capture-MS Homo sapiens
74 RAE1 8480
Affinity Capture-MS Homo sapiens
75 SUMO1 7341
Affinity Capture-MS Homo sapiens
76 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
77 SIRT6  
Affinity Capture-MS Homo sapiens
78 NUP205 23165
Affinity Capture-MS Homo sapiens
79 PDGFD 80310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 NUP133 55746
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
81 TMEM214 54867
Affinity Capture-MS Homo sapiens
82 VIM 7431
Co-fractionation Homo sapiens
83 RPA1 6117
Affinity Capture-MS Homo sapiens
84 SQSTM1 8878
Proximity Label-MS Homo sapiens
85 ACTR1B 10120
Co-fractionation Homo sapiens
86 DCTN2 10540
Co-fractionation Homo sapiens
87 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
88 NUP35 129401
Affinity Capture-MS Homo sapiens
89 DDX58 23586
Affinity Capture-RNA Homo sapiens
90 NUP98 4928
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
91 CCT7 10574
Affinity Capture-MS Homo sapiens
92 Nup214  
Affinity Capture-MS Mus musculus
93 NUP107 57122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
94 MIOS 54468
Affinity Capture-MS Homo sapiens
95 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
96 BECN1 8678
Affinity Capture-MS Homo sapiens
97 NDC1 55706
Affinity Capture-MS Homo sapiens
98 RAB7A 7879
Proximity Label-MS Homo sapiens
99 Rrbp1  
Affinity Capture-MS Mus musculus
100 WDR59 79726
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
101 SERBP1 26135
Affinity Capture-MS Homo sapiens
102 SNW1 22938
Affinity Capture-MS Homo sapiens
103 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
104 C9orf72  
Affinity Capture-MS Homo sapiens
105 MYC  
Affinity Capture-MS Homo sapiens
106 NUP85 79902
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
107 C16orf71  
Affinity Capture-MS Homo sapiens
108 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
109 RANGAP1 5905
Affinity Capture-MS Homo sapiens
110 RANBP2 5903
Affinity Capture-MS Homo sapiens
111 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SEH1L is involved
PathwayEvidenceSource
Amino acids regulate mTORC1 TAS Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress TAS Reactome
Cellular response to starvation TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Prophase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope (NE) Reassembly TAS Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation TAS Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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