Gene description for QPRT
Gene name quinolinate phosphoribosyltransferase
Gene symbol QPRT
Other names/aliases HEL-S-90n
QPRTase
Species Homo sapiens
 Database cross references - QPRT
ExoCarta ExoCarta_23475
Vesiclepedia VP_23475
Entrez Gene 23475
HGNC 9755
MIM 606248
UniProt Q15274  
 QPRT identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for QPRT
Molecular Function
    nicotinate-nucleotide diphosphorylase (carboxylating) activity GO:0004514 IBA
    nicotinate-nucleotide diphosphorylase (carboxylating) activity GO:0004514 IDA
    nicotinate-nucleotide diphosphorylase (carboxylating) activity GO:0004514 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    NAD biosynthetic process GO:0009435 IBA
    NAD biosynthetic process GO:0009435 IEA
    NAD metabolic process GO:0019674 TAS
    quinolinate catabolic process GO:0034213 IBA
    quinolinate catabolic process GO:0034213 IDA
    quinolinate catabolic process GO:0034213 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
    catalytic complex GO:1902494 IDA
 Experiment description of studies that identified QPRT in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for QPRT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAVER2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CCAR1 55749
Affinity Capture-MS Homo sapiens
3 TNS4 84951
Affinity Capture-MS Homo sapiens
4 SUFU 51684
Two-hybrid Homo sapiens
5 EIF4G3 8672
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TRAF3IP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GATAD2B 57459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PER1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GBX1  
Affinity Capture-MS Homo sapiens
10 CTAGE5 4253
Affinity Capture-MS Homo sapiens
11 JMJD1C 221037
Affinity Capture-MS Homo sapiens
12 SATB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 KRTAP1-1  
Two-hybrid Homo sapiens
14 SMARCD1 6602
Affinity Capture-MS Homo sapiens
15 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 ADAMTSL4  
Two-hybrid Homo sapiens
17 GIT2 9815
Two-hybrid Homo sapiens
18 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 NLGN3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 UBA1 7317
Co-fractionation Homo sapiens
22 TLE1 7088
Affinity Capture-MS Homo sapiens
23 MGARP  
Co-fractionation Homo sapiens
24 TRIM33 51592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CRY1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ADNP 23394
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 GRPEL1 80273
Co-fractionation Homo sapiens
29 NPL 80896
Affinity Capture-MS Homo sapiens
30 ANKRD17 26057
Affinity Capture-MS Homo sapiens
31 QPRT 23475
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 SCAF4 57466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KRTAP10-3  
Two-hybrid Homo sapiens
34 SMARCD2 6603
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CPSF7 79869
Affinity Capture-MS Homo sapiens
36 AARSD1 80755
Affinity Capture-MS Homo sapiens
37 TLE4  
Affinity Capture-MS Homo sapiens
38 ESR1  
Affinity Capture-MS Homo sapiens
39 SF3A1 10291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TRIM24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ACTR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 DMAP1 55929
Affinity Capture-MS Homo sapiens
43 ALDH18A1 5832
Co-fractionation Homo sapiens
44 KRTAP4-12  
Two-hybrid Homo sapiens
45 IRF2BP1 26145
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 HDAC3 8841
Affinity Capture-MS Homo sapiens
47 SRCAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 ANKHD1 54882
Affinity Capture-MS Homo sapiens
49 VPS72  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 NCOR2  
Affinity Capture-MS Homo sapiens
51 ZMYM6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 IARS2 55699
Co-fractionation Homo sapiens
53 TBL1XR1 79718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ARID1B 57492
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SPAG9 9043
Affinity Capture-MS Homo sapiens
56 MIA3 375056
Affinity Capture-MS Homo sapiens
57 ZMYM4  
Affinity Capture-MS Homo sapiens
58 PATL1 219988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 GOPC 57120
Affinity Capture-MS Homo sapiens
60 KRT40  
Two-hybrid Homo sapiens
61 PRRC2C 23215
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CRY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 EIF4G2 1982
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SEH1L 81929
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SMURF2 64750
Affinity Capture-Western Homo sapiens
66 FKBP10 60681
Co-fractionation Homo sapiens
67 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 BCKDHA 593
Co-fractionation Homo sapiens
69 SKIL  
Affinity Capture-MS Homo sapiens
70 MPND  
Affinity Capture-MS Homo sapiens
71 RCOR1  
Affinity Capture-MS Homo sapiens
72 ALDH2 217
Co-fractionation Homo sapiens
73 NCOR1  
Affinity Capture-MS Homo sapiens
74 SF3A3 10946
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 U2SURP 23350
Affinity Capture-MS Homo sapiens
76 LACTB 114294
Affinity Capture-MS Homo sapiens
77 SATB1 6304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 BCOR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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